Recombinant Human TRAILR1/TNFRSF10A Fc Chimera Protein, CF

Catalog #: 6949-DR Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
6949-DR-100

R&D Systems Recombinant Proteins and Enzymes

1 Image

All TRAILR1/TNFRSF10A Proteins and Enzymes

Product Details

Supplemental Products

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human TRAILR1/TNFRSF10A Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit TRAIL-mediated cytotoxicity using L‑929 mouse fibroblast cells treated with TRAIL. The ED₅₀ for this effect is 0.25-1.25 ng/mL in the presence of 6 ng/mL of Recombinant Human TRAIL/TNFSF10 (Catalog # 375-TL).

Source

Chinese Hamster Ovary cell line, CHO-derived human TRAILR1/TNFRSF10A protein

Human TRAIL R1 (Ala109-Asn239) Accession # AAC51226	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

AAC51226

N-terminal Sequence
Analysis

Ala109

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

40.9 kDa (monomer)

SDS-PAGE

50-55 kDa, reducing conditions

Product Datasheets

6949-DR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6949-DR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: TRAILR1/TNFRSF10A

Human TRAIL R1 (TNF-related apoptosis inducing ligand receptor 1), also called DR4 (death receptor 4), is a 50-57 kDa, 468 amino acid (aa) type 1 transmembrane protein in the TNF R family, designated TNFRSF10A (1-3). Of the five receptors for TRAIL (also called APO2 ligand) in humans, TRAIL R1/DR4 and TRAIL R2/DR5 are apoptosis-inducing and share 59% aa identity within the cytoplasmic death domain (aa 365 ‑ 448 of human TRAIL R1) (1-3). Rodents do not express TRAIL R1/DR4. Human decoy receptors TRAIL R3 and TRAIL R4 have non-functional death domains but share ~60% aa identity with TRAIL R1 within extracellular TNFR domains (aa 107-229), while the secreted TRAIL receptor osteoprotegerin shares only 18% aa identity with TRAIL R1. Trimeric TRAIL engagement of TRAIL R1 induces apoptosis by recruiting a cytosolic death-inducing signaling complex (DISC) that includes the adaptor FADD and caspases 8 and 10 (2-6). TRAIL R1 translational modifications, such as O-glycosylation and palmitoylation, promote apoptosis by enhancing oligomerization and presence in lipid rafts, respectively (3-6). Recombinant human TRAIL R1 neutralizes the ability of TRAIL to induce apoptosis (1). TRAIL R1 protein is expressed mainly in damaged, infected and malignant cells and can be repressed by hedgehog proteins in oncogenic cells (3, 7, 8). TRAIL R1 is also expressed on hemopoietic cells and influences activities such as monocyte migration (9). On senescent neutrophils, CXCL12/SDF-1 engagement of CXCR4 induces expression of all TRAIL R (7). TRAIL functions in immune surveillance, inducing apoptosis in cancer cells but not normal cells (3, 10, 11). TRAIL R1 surface expression correlates more closely than that of TRAIL R2 with TRAIL induction of apoptosis on tumor cells such as melanoma and chronic lymphocytic and acute myeloid leukemias (6, 12, 13).

References

Pan, G. et al. (1997) Science 276:111.
Zauli, G. and P. Secchiero (2006) Cytokine Growth Factor Rev. 17:245.
Yang, A. et al. (2010) Curr. Opin. Cell Biol. 22:837.
Rossin, A. et al. (2009) Biohem. J. 419:185.
Psahoulia, F.H. et al. (2007) Mol. Cancer Ther. 6:2591.
Ries, C.R. et al. (2010) Cell Death Dis. 1:e83.
Lum, J.J. et al. (2005) J. Immunol. 175:1232.
Kurita, S. et al. (2010) Oncogene 29:4848.
Wei, W. et al. (2010) Mol. Immunol. 47:2475.
Sung, E-S. et al. (2009) Mol. Cancer Ther. 8:2276.
Mom, C.H. et al. (2009) Clin. Cancer Res. 15:5584.
MacFarlane, M. et al. (2005) Cell Death Differ. 12:773.
Kurbanov, B.M. et al. (2005) J. Invest. Dermatol. 125:1010.

Long Name

TRAIL Receptor 1

Entrez Gene IDs

8797 (Human)

Alternate Names

APO2; CD261 antigen; CD261; cytotoxic TRAIL receptor; Death receptor 4; DR4 TRAIL receptor 1; DR4; TNF-related apoptosis inducing ligand receptor 1; TNF-related apoptosis-inducing ligand receptor 1; TNFRSF10A; TRAIL R1; TRAILR1; TRAIL-R1; TRAILR-1; TRAILR1MGC9365; tumor necrosis factor receptor superfamily member 10A; tumor necrosis factor receptor superfamily, member 10a