Recombinant Human TrkA His Tagged Protein, CF

Catalog #: 9966-TK Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9966-TK-050

Recombinant Human TrkA His Tagged Protein Bioactivity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human TrkA His Tagged Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit NGF-induced proliferation of TF‑1 human erythroleukemic cells. The ED₅₀ for this effect is 0.2-1.2 µg/mL in the presence of 10 ng/mL of Recombinant Human beta-NGF (Catalog # 256-GF).

Source

Human embryonic kidney cell, HEK293-derived human TrkA protein
Ala34-Gly423, with a C-terminal 6-His tag

Accession #

P04629-1

N-terminal Sequence
Analysis

Ala34

Predicted Molecular Mass

43 kDa

SDS-PAGE

86-100 kDa, reducing conditions

Product Datasheets

9966-TK

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9966-TK

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 1 mg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	12 months from date of receipt, ≤ -20 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Human TrkA inhibits Recombinant Human beta-NGF-induced proliferation of TF‑1 human erythroleukemic cells. The ED₅₀ for this effect is 0.2-1.2 μg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human TrkA was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 86-100 kDa.

Reconstitution Calculator

Background: TrkA

TrkA (Tyrosine kinase receptor A), also known as High affinity NGF receptor, is a member of the neurotrophic tyrosine kinase receptor family that has three members, TrkA, Trk B and Trk C, which preferentially bind NGF, NT-4 and BDNF, and NT-3, respectively (1). All Trk family proteins share a conserved complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain. Two distinct TrkA isoforms that differ by virtue of a 6-amino acid insertion in their extracellular domain have been identified. The longer TrkA isoform is the only isoform expressed within neuronal tissues whereas the shorter TrkA is expressed mainly in non-neuronal tissues (1). NGF binds to TrkA with low affinity and activates its cytoplasmic kinase, initiating a signaling cascade that mediates neuronal survival and differentiation. Higher affinity binding of NGF requires the coexpression of TrkA with the p75 NGF receptor (NGFR), a member of the tumor necrosis factor receptor superfamily (2). NGFR binds all neurotrophins with low affinity and modulates Trk activity as well as alters the specificity of Trk receptors for their ligands. NGFR can also mediate cell death when being expressed independent of Trk (3).

References

Shelton D.L. et al. (1995) J. Neurosci. 15:477.
Esposito, D. et al. (2001) J. Biol. Chem. 276:32687.
Sofroniew, M.V. et al. (2001) Annu. Rev. Neurosci. 24:1217.

Long Name

Neurotrophic Tyrosine Kinase Receptor A

Entrez Gene IDs

4914 (Human); 18211 (Mouse); 59109 (Rat)

Alternate Names

DKFZp781I14186; EC 2.7.10; EC 2.7.10.1; MTChigh affinity nerve growth factor receptor; Neurotrophic tyrosine kinase receptor type 1; neurotrophic tyrosine kinase, receptor, type 1; NTRK1; NTRK-1; p140-TrkA; TRK1-transforming tyrosine kinase protein; TrkA; Trk-A; TRKAOncogene TRK; TRKTRK1; tyrosine kinase receptor A