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Recombinant Human UbcH5b/UBE2D2 Protein, CF

Catalog #: E2-622
45 Citations 1 Review Datasheet / COA / SDS
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E2-622-100
R&D Systems Recombinant Proteins and Enzymes
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Citations (45)
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Reviews (1)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human UbcH5b/UBE2D2 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Recombinant Human UbcH5b/UBE2D2 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human UbcH5b/UBE2D2 concentration of 0.1-1 μM.
Source
E. coli-derived human UbcH5b/UBE2D2 protein
Met1 - Met147
Accession #
P62837.1
Predicted Molecular Mass
17 kDa

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E2-622

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COA
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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E2-622

Formulation Supplied as a solution in HEPES, NaCl, TCEP and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
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Background: UbcH5b/UBE2D2

Ubiquitin-conjugating Enzyme H5b (UbcH5b), also known as Ubiquitin-conjugating Enzyme E2D 2 (UBE2D2), is a widely expressed member of the Ubiquitin-conjugating (E2) enzyme family (1). The protein has a predicted molecular weight of 16.5 kDa. UbcH5b/UBE2D2 localizes to both the nucleus and the cytoplasm (2). The human protein shares 100% and 92% amino acid sequence identity with the mouse and rat orthologs, respectively. This enzyme has an E2 catalytic core domain that contains an active site cysteine residue that is required for the formation of a thioester bond with Ubiquitin (3). UbcH5b/UBE2D2 is capable of mediating the formation of Ubiquitin chains linked through Lys11, Lys48, or Lys63 (4). Working with the SCF(Fbxw2) and MDM2/HDM2 Ubiquitin ligases (E3s), UbcH5b/UBE2D2 mediates the ubiquitination and degradation of the transcription factors GCM1 and p53, respectively (5-7). Along with UBE2N/Ubc13, UbcH5b/UBE2D2 may have a role in the endocytosis and endolysosomal degradation of MHC class I molecules (8). Non-proteolytic ubiquitination of TRIM5-alpha by UbcH5b/UBE2D2 has been reported to block HIV reverse transcription (9). Pathologically, UBE2D family members may be critical targets of cadmium during cadmium-induced renal toxicity (10). Additionally, overexpression of UbcH5b/UBE2D2 has been linked to inflammatory bowel disease (11).

References
  1. Jensen, J.P. et al. (1995) J. Biol. Chem. 270:30408.
  2. Umebayashi, K. et al. (2008) Mol. Biol. Cell 19:3454.
  3. Hershko, A. et al. (1983) J. Biol. Chem. 258:8206.
  4. Ordureau, A. et al. (2008) Biochem. J. 409:43.
  5. Chiang, M.H. et al. (2008) Biol. Reprod. 79:914.
  6. Saville, M.K. et al. (2004) J. Biol. Chem. 279:42169.
  7. Iyappan, S. et al. (2010) J. Biol. Chem. 285:33065.
  8. Duncan, L.M. et al. (2006) EMBO J. 25:1635.
  9. Yamauchi, K. et al. (2008) FEBS J. 275:1540.
  10. Tokumoto, M. et al. (2011) J. Toxicol. Sci. 36:191.
  11. Hetzenecker, A.M. et al. (2012) Digestion 86:34.
Entrez Gene IDs
7322 (Human); 56550 (Mouse); 641452 (Rat)
Alternate Names
E2(17)KB2; EC 6.3.2.19; PUBC1; UBC4/5; UBC4ubiquitin-conjugating enzyme E2D 2 (homologous to yeast UBC4/5); UBC5B; UBCH4; UbcH5b; UBE2D2; Ubiquitin carrier protein D2; ubiquitin-conjugating enzyme E2 D2; Ubiquitin-conjugating enzyme E2(17)KB 2; Ubiquitin-conjugating enzyme E2-17 kDa 2; ubiquitin-conjugating enzyme E2D 2 (UBC4/5 homolog, yeast); Ubiquitin-protein ligase D2

Citations for Recombinant Human UbcH5b/UBE2D2 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

45 Citations: Showing 1 - 10
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  1. The RING-finger ubiquitin E3 ligase TaPIR1 targets TaHRP1 for degradation to suppress chloroplast function
    Authors: Zhang, R;Wu, Y;Qu, X;Yang, W;Wu, Q;Huang, L;Jiang, Q;Ma, J;Zhang, Y;Qi, P;Chen, G;Jiang, Y;Zheng, Y;Wang, X;Wei, Y;Xu, Q;
    Nature communications
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay
  2. Lipid-mediated intracellular delivery of recombinant bioPROTACs for the rapid degradation of undruggable proteins
    Authors: Chan, A;Haley, RM;Najar, MA;Gonzalez-Martinez, D;Bugaj, LJ;Burslem, GM;Mitchell, MJ;Tsourkas, A;
    Nature communications
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Targeting DCAF5 suppresses SMARCB1-mutant cancer by stabilizing SWI/SNF
    Authors: Radko-Juettner, S;Yue, H;Myers, JA;Carter, RD;Robertson, AN;Mittal, P;Zhu, Z;Hansen, BS;Donovan, KA;Hunkeler, M;Rosikiewicz, W;Wu, Z;McReynolds, MG;Roy Burman, SS;Schmoker, AM;Mageed, N;Brown, SA;Mobley, RJ;Partridge, JF;Stewart, EA;Pruett-Miller, SM;Nabet, B;Peng, J;Gray, NS;Fischer, ES;Roberts, CWM;
    Nature
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay
  4. Structure of the p53 degradation complex from HPV16
    Authors: Wang, JCK;Baddock, HT;Mafi, A;Foe, IT;Bratkowski, M;Lin, TY;Jensvold, ZD;Preciado López, M;Stokoe, D;Eaton, D;Hao, Q;Nile, AH;
    Nature communications
    Species: Bacteria - Escherichia coli
    Sample Types: Whole Cells
    Applications: Bioassay
  5. The MYC2-PUB22-JAZ4 module plays a crucial role in jasmonate signaling in tomato
    Authors: Wu, S;Hu, C;Zhu, C;Fan, Y;Zhou, J;Xia, X;Shi, K;Zhou, Y;Foyer, CH;Yu, J;
    Molecular plant
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay
  6. UV-B irradiation-activated E3 ligase GmILPA1 modulates gibberellin catabolism to increase plant height in soybean
    Authors: Sun, J;Huang, S;Lu, Q;Li, S;Zhao, S;Zheng, X;Zhou, Q;Zhang, W;Li, J;Wang, L;Zhang, K;Zheng, W;Feng, X;Liu, B;Kong, F;Xiang, F;
    Nature communications
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay
  7. HAPSTR1 localizes HUWE1 to the nucleus to limit stress signaling pathways
    Authors: Monda, JK;Ge, X;Hunkeler, M;Donovan, KA;Ma, MW;Jin, CY;Leonard, M;Fischer, ES;Bennett, EJ;
    Cell reports
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay
  8. Calpain activity is negatively regulated by a KCTD7-Cullin-3 complex via non-degradative ubiquitination
    Authors: J Sharma, S Mulherkar, UI Chen, Y Xiong, L Bajaj, BK Cho, YA Goo, HE Leung, KF Tolias, M Sardiello
    Cell Discovery, 2023-03-24;9(1):32.
    Species: Human
    Sample Types: Whole Cells
    Applications: Ubiquitination
  9. TRIM21-dependent target protein ubiquitination mediates cell-free Trim-Away
    Authors: TET Mevissen, AV Prasad, JC Walter
    Cell Reports, 2023-02-18;42(2):112125.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay
  10. Calpain activity is negatively regulated by a KCTD7-Cullin-3 complex via non-degradative ubiquitination
    Authors: J Sharma, S Mulherkar, UI Chen, Y Xiong, L Bajaj, BK Cho, YA Goo, HE Leung, KF Tolias, M Sardiello
    Cell Discovery, 2023;9(1):32.
    Species: Human
    Sample Types: Whole Cells
    Applications: Ubiquitination
  11. SNRPD2 Is a Novel Substrate for the Ubiquitin Ligase Activity of the Salmonella Type III Secretion Effector SlrP
    Authors: A Bullones-B, JL Araujo-Gar, J Fernández-, F Romero, J Bernal-Bay, F Ramos-Mora
    Biology, 2022-10-17;11(10):.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay
  12. Host E3 ligase HUWE1 attenuates the pro-apoptotic activity of the MERS-CoV accessory protein ORF3 by promoting its ubiquitin-dependent degradation
    Authors: Y Zhou, R Zheng, S Liu, C Disoma, A Du, S Li, Z Chen, Z Dong, Y Zhang, S Li, P Liu, A Razzaq, X Chen, Y Liao, S Tao, Y Liu, L Xu, Q Zhang, J Peng, X Deng, S Li, T Jiang, Z Xia
    The Journal of Biological Chemistry, 2022-01-13;0(0):101584.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  13. Structure of CRL2Lrr1, the E3 ubiquitin ligase that promotes DNA replication termination in vertebrates
    Authors: H Zhou, MS Zaher, JC Walter, A Brown
    Nucleic Acids Research, 2021-12-16;49(22):13194-13206.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay
  14. SARS-CoV-2 Nsp5 Demonstrates Two Distinct Mechanisms Targeting RIG-I and MAVS To Evade the Innate Immune Response
    Authors: Y Liu, C Qin, Y Rao, C Ngo, JJ Feng, J Zhao, S Zhang, TY Wang, J Carriere, AC Savas, M Zarinfar, S Rice, H Yang, W Yuan, JA Camarero, J Yu, XS Chen, C Zhang, P Feng
    MBio, 2021-09-21;0(0):e0233521.
    Species: E. coli, Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  15. Structural basis of human transcription-DNA repair coupling
    Authors: G Kokic, FR Wagner, A Chernev, H Urlaub, P Cramer
    Nature, 2021-09-15;0(0):.
    Species: Human
    Sample Types: Recombinant Proteins
    Applications: Bioassay
  16. DAZAP2 acts as specifier of the p53 response to DNA damage
    Authors: MC Liebl, J Moehlenbri, H Becker, G Raddatz, SK Abdeen, RI Aqeilan, F Lyko, TG Hofmann
    Nucleic Acids Research, 2021-03-18;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Ubiquitination
  17. CHIP ubiquitylates NOXA and induces its lysosomal degradation in response to DNA damage
    Authors: MC Albert, K Brinkmann, W Pokrzywa, SD Günther, M Krönke, T Hoppe, H Kashkar
    Cell Death & Disease, 2020-09-10;11(9):740.
    Species: Human
    Sample Types: Whole Cells
    Applications: Ubiquitylation
  18. Alternative splicing and allosteric regulation modulate the chromatin binding of UHRF1
    Authors: M Tauber, S Kreuz, A Lemak, P Mandal, Z Yerkesh, A Veluchamy, B Al-Gashgar, A Aljahani, LV Cortés-Med, D Azhibek, L Fan, MS Ong, S Duan, S Houliston, CH Arrowsmith, W Fischle
    Nucleic Acids Res., 2020-08-20;0(0):.
    Species: Human
    Sample Types: Protein
    Applications: Ubiquitination
  19. Targeting a helix-in-groove interaction between E1 and E2 blocks ubiquitin transfer
    Authors: AM Cathcart, GH Bird, TE Wales, HD Herce, EP Harvey, ZJ Hauseman, CE Newman, U Adhikary, MS Prew, T Oo, S Lee, JR Engen, LD Walensky
    Nat. Chem. Biol., 2020-08-17;0(0):.
    Species: Human, N/A
    Sample Types: Peptide, Protein
    Applications: Bioassay
  20. PHF7 Modulates BRDT Stability and Histone-to-Protamine Exchange during Spermiogenesis
    Authors: CR Kim, T Noda, H Kim, G Kim, S Park, Y Na, S Oura, K Shimada, I Bang, JY Ahn, YR Kim, SK Oh, HJ Choi, JS Kim, I Jung, H Lee, Y Okada, M Ikawa, SH Baek
    Cell Rep, 2020-07-28;32(4):107950.
    Species: Mouse
    Sample Types: Protein
    Applications: Bioassay
  21. Mitochondria ubiquitin ligase, MARCH5 resolves hepatitis B virus X protein aggregates in the liver pathogenesis
    Authors: YS Yoo, YJ Park, HS Lee, NTK Oanh, MY Cho, J Heo, ES Lee, H Cho, YY Park, H Cho
    Cell Death Dis, 2019-12-09;10(12):938.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  22. Aryl Sulfonamides Degrade RBM39 and RBM23 by Recruitment to CRL4-DCAF15
    Authors: TC Ting, M Goralski, K Klein, B Wang, J Kim, Y Xie, D Nijhawan
    Cell Rep, 2019-11-05;29(6):1499-1510.e6.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  23. RNF126 Quenches RNF168 Function in the DNA Damage Response
    Authors: L Zhang, Z Wang, R Shi, X Zhu, J Zhou, B Peng, X Xu
    Genomics Proteomics Bioinformatics, 2018-12-04;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  24. The E3 ubiquitin ligase Siah-1 suppresses avian reovirus infection by targeting p10 for degradation
    Authors: X Chen, Z He, M Fu, Y Wang, H Wu, X Li, H Cao, SJ Zheng
    J. Virol., 2018-02-26;0(0):.
    Applications: Bioassay
  25. Impaired oxidative stress response characterizes HUWE1-promoted X-linked intellectual disability
    Authors: M Bosshard, R Aprigliano, C Gattiker, V Palibrk, E Markkanen, P Hoff Backe, S Pellegrino, FL Raymond, G Froyen, M Altmeyer, M Bjørås, GL Dianov, B van Loon
    Sci Rep, 2017-11-08;7(1):15050.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  26. COP1 mediates dark-specific degradation of microtubule-associated protein WDL3 in regulating Arabidopsis hypocotyl elongation
    Authors: N Lian, X Liu, X Wang, Y Zhou, H Li, J Li, T Mao
    Proc. Natl. Acad. Sci. U.S.A., 2017-10-30;114(46):12321-12326.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  27. BLADE-ON-PETIOLE proteins act in an E3 ubiquitin ligase complex to regulate PHYTOCHROME INTERACTING FACTOR 4 abundance
    Authors: B Zhang, M Holmlund, S Lorrain, M Norberg, L Bakó, C Fankhauser, O Nilsson
    Elife, 2017-08-22;6(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  28. The Banana Fruit SINA Ubiquitin Ligase MaSINA1 Regulates the Stability of MaICE1 to be Negatively Involved in Cold Stress Response
    Authors: ZQ Fan, JY Chen, JF Kuang, WJ Lu, W Shan
    Front Plant Sci, 2017-06-12;8(0):995.
    Applications: Bioassay
  29. The Ubiquitin Ligase CHIP Integrates Proteostasis and Aging by Regulation of Insulin Receptor Turnover
    Authors: R Tawo, W Pokrzywa, É Kevei, ME Akyuz, V Balaji, S Adrian, J Höhfeld, T Hoppe
    Cell, 2017-04-20;169(3):470-482.e13.
    Applications: Bioassay
  30. Reciprocal proteasome-mediated degradation of PIFs and HFR1 underlying photomorphogenic development in Arabidopsis
    Authors: X Xu, PK Kathare, VN Pham, Q Bu, A Nguyen, E Huq
    Development, 2017-04-18;0(0):.
    Applications: Bioassay
  31. Smurf1 inhibits integrin activation by controlling Kindlin-2 ubiquitination and degradation
    Authors: X Wei, X Wang, J Zhan, Y Chen, W Fang, L Zhang, H Zhang
    J. Cell Biol., 2017-04-13;0(0):.
    Applications: Bioassay
  32. Epilepsy-associated gene Nedd4-2 mediates neuronal activity and seizure susceptibility through AMPA receptors
    Authors: J Zhu, KY Lee, KA Jewett, HY Man, HJ Chung, NP Tsai
    PLoS Genet, 2017-02-17;13(2):e1006634.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  33. Fine-tuning of ULK1 mRNA and protein levels is required for autophagy oscillation
    Authors: Francesca Nazio
    J. Cell Biol, 2016-12-08;215(6):841-856.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  34. Identification and Characterization of MCM3 as a Novel KEAP1 Substrate
    J Biol Chem, 2016-09-12;0(0):.
    Species: Human
    Sample Types: Protein
    Applications: Ubiquitination
  35. Role of SUMO activating enzyme in cancer stem cell maintenance and self-renewal
    Nat Commun, 2016-07-28;7(0):12326.
    Applications: Bioassay
  36. PTP1B controls non-mitochondrial oxygen consumption by regulating RNF213 to promote tumour survival during hypoxia
    Authors: RS Banh, C Iorio, R Marcotte, Y Xu, D Cojocari, AA Rahman, J Pawling, W Zhang, A Sinha, CM Rose, M Isasa, S Zhang, R Wu, C Virtanen, T Hitomi, T Habu, SS Sidhu, A Koizumi, SE Wilkins, T Kislinger, SP Gygi, CJ Schofield, JW Dennis, BG Wouters, BG Neel
    Nat. Cell Biol, 2016-06-20;18(7):803-13.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Ubiquitination
  37. 14-3-3? loss leads to activation of the epithelial to mesenchymal transition due to the stabilization of c-Jun
    Authors: Kumarkrishna Raychaudhu
    J Biol Chem, 2016-06-03;0(0):.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  38. Phosphorylation of CHIP at Ser20 by Cdk5 promotes tAIF-mediated neuronal death.
    Authors: Kim C, Yun N, Lee J, Youdim M, Ju C, Kim W, Han P, Oh Y
    Cell Death Differ, 2015-07-24;23(2):333-46.
    Applications: Enzyme Assay
  39. Vaccinia-related kinase 2 mediates accumulation of polyglutamine aggregates via negative regulation of the chaperonin TRiC.
    Authors: Kim, Sangjune, Park, Do-Young, Lee, Dohyun, Kim, Wanil, Jeong, Young-Hu, Lee, Juhyun, Chung, Sung-Kee, Ha, Hyunjung, Choi, Bo-Hwa, Kim, Kyong-Ta
    Mol Cell Biol, 2013-12-02;34(4):643-52.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  40. The E3 ubiquitin ligases RNF126 and Rabring7 regulate endosomal sorting of the epidermal growth factor receptor.
    Authors: Smith, Christop, Berry, Donna M, McGlade, C Jane
    J Cell Sci, 2013-02-15;126(0):1366-80.
    Applications: Bioassay
  41. Characterization of ML-IAP protein stability and physiological role in vivo.
    Authors: Varfolomeev, Eugene, Moradi, Elham, Dynek, Jasmin N, Zha, Jiping, Fedorova, Anna V, Deshayes, Kurt, Fairbrother, Wayne J, Newton, Kim, Le Couter, Jennifer, Vucic, Domagoj
    Biochem J, 2012-11-01;447(3):427-36.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  42. The F-box protein FBXO44 mediates BRCA1 ubiquitination and degradation.
    Authors: Lu, Yunzhe, Li, Jiezhi, Cheng, Dongmei, Parameswaran, Balaji, Zhang, Shaohua, Jiang, Zefei, Yew, P Renee, Peng, Junmin, Ye, Qinong, Hu, Yanfen
    J Biol Chem, 2012-10-18;287(49):41014-22.
    Applications: Ubiquitination
  43. HDM2 antagonist MI-219 (spiro-oxindole), but not Nutlin-3 (cis-imidazoline), regulates p53 through enhanced HDM2 autoubiquitination and degradation in human malignant B-cell lymphomas.
    J Hematol Oncol, 2012-09-18;5(0):57.
    Applications: Bioassay
  44. DNA damage-induced heterogeneous nuclear ribonucleoprotein K sumoylation regulates p53 transcriptional activation.
    Authors: Pelisch F, Pozzi B, Risso G, Munoz M, Srebrow A
    J Biol Chem, 2012-07-23;287(36):30789-99.
    Applications: Bioassay
  45. Functional analysis of NopM, a novel E3 ubiquitin ligase (NEL) domain effector of Rhizobium sp. strain NGR234.
    Authors: Xin DW, Liao S, Xie ZP
    PLoS Pathog., 2012-05-17;8(5):e1002707.

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Recombinant Human UbcH5b/UBE2D2 Protein, CF
By Patrick Chuong on 01/27/2020
Application: Enzymatic activity in vitro
Enzymatic activity in vitro Recombinant Human UbcH5b/UBE2D2 Protein, CF E2-622