Recombinant Human ULBP-3 Fc Chimera Protein, CF Summary
Product Specifications
| Human ULBP-3 (Gly27-Pro216 & His32-Pro216) Accession # NP_078794 |
IEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1517-UL
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: ULBP-3
ULBP-3 is a member of a family of cell-surface proteins that function as ligands for human NKG2D. ULBP-3 has also been described under the names RaeT1N (retinoic acid early transcript), NKG2DL3, and ALCAN-gamma. The name ULBP-3 derives from the original identification of three proteins, ULBP-1, -2, and -3, as ligands for the human cytomegalovirus glycoprotein UL16; they were designated UL16 binding proteins (ULBP). The gene for ULBP-3 resides in a cluster of ten related genes, six of which encode potentially functional glycoproteins. Amino acid sequence identity within this family ranges from 30-60%. These proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig-like domains, and they have no capacity to bind peptide or interact with beta 2‑microglobulin. Some family members, including ULBP-3, are anchored to the membrane via a GPI-linkage, whereas others have transmembrane domains. ULBP-3 and several other family members are known to bind to human NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8+ alpha beta T cells. Engagement of NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. The ULBPs are expressed on some tumor cells and have been implicated in tumor surveillance (1-7).
- Cosman, D. et al. (2001) Immunity 14:123.
- Kubin, M. et al. (2001) Eur. J. Immunol. 31:1428.
- Sutherland, C. et al. (2002) J. Immunol. 168:671.
- Steinle, A. et al. (2001) Immunogenetics 53:279.
- Sutherland, C. et al. (2001) Immunol. Rev. 181:185.
- Pende, D. et al. (2002) Cancer Res. 62:6178.
- Radosavljevic, M. et al. (2002) Genomics 79:114.
- NKG2D and its Ligands (2002) www.RnDSystems.com.
Citations for Recombinant Human ULBP-3 Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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Soluble NKG2DLs Are Elevated in Breast Cancer Patients and Associate with Disease Outcome
Authors: Seller, A;Tegeler, CM;Mauermann, J;Schreiber, T;Hagelstein, I;Liebel, K;Koch, A;Heitmann, JS;Greiner, SM;Hayn, C;Dannehl, D;Engler, T;Hartkopf, AD;Hahn, M;Brucker, SY;Salih, HR;Märklin, M;
International journal of molecular sciences
Species: Human
Sample Types: Serum
Applications: ELISA Standard -
Arming oHSV with ULBP3 drives abscopal immunity in lymphocyte-depleted glioblastoma
Authors: HG Wirsching, H Zhang, F Szulzewsky, S Arora, P Grandi, PJ Cimino, N Amankulor, JS Campbell, L McFerrin, SS Pattwell, C Ene, A Hicks, M Ball, J Yan, J Zhang, D Kumasaka, RH Pierce, M Weller, M Finer, C Quéva, JC Glorioso, AM Houghton, EC Holland
JCI Insight, 2019-07-11;4(13):.
Species: Human, Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Increasing TIMP3 expression by hypomethylating agents diminishes soluble MICA, MICB and ULBP2 shedding in acute myeloid leukemia, facilitating NK cell-mediated immune recognition
Authors: AB Raneros, AM Puras, RM Rodriguez, E Colado, T Bernal, E Anguita, AV Mogorron, AC Gil, JR Vidal-Cast, L Márquez-Ki, PD Bulnes, AM Marin, MCG Garay, B Suarez-Alv, C Lopez-Larr
Oncotarget, 2017-05-09;8(19):31959-31976.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA (Standard) -
Natural Killer Group 2D Ligand Depletion Reconstitutes Natural Killer Cell Immunosurveillance of Head and Neck Squamous Cell Carcinoma
Authors: S Weil, S Memmer, A Lechner, V Huppert, A Giannattas, T Becker, A Müller-Run, K Lampe, D Beutner, A Quaas, R Schubert, E Herrmann, A Steinle, U Koehl, L Walter, MS von Bergwe, J Koch
Front Immunol, 2017-04-10;8(0):387.
Applications: Bioassay -
Cytotoxicity and infiltration of human NK cells in in vivo-like tumor spheroids.
Authors: Giannattasio A, Weil S, Kloess S, Ansari N, Stelzer E, Cerwenka A, Steinle A, Koehl U, Koch J
BMC Cancer, 2015-05-03;15(0):351.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA (Standard) -
The regulatory effect of UL-16 binding protein-3 expression on the cytotoxicity of NK cells in cancer patients.
Authors: Mou, Xiao, Zhou, Yuepeng, Jiang, Peng, Zhou, Tong, Jiang, Qian, Xu, Chengche, Liu, Hongli, Zheng, Tingting, Yuan, Guoyue, Zhang, Yanyun, Chen, Deyu, Mao, Chaoming
Sci Rep, 2014-08-20;4(0):6138.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
The prognostic significance of soluble NKG2D ligands in B-cell chronic lymphocytic leukemia.
Authors: Nuckel H, Switala M, Sellmann L, Horn PA, Durig J, Duhrsen U, Kuppers R, Grosse-Wilde H, Rebmann V
Leukemia, 2010-04-29;24(6):1152-9.
Applications: ELISA (Standard) -
Biologic activity of irradiated, autologous, GM-CSF-secreting leukemia cell vaccines early after allogeneic stem cell transplantation.
Authors: Ho VT, Vanneman M, Kim H, Sasada T, Kang YJ, Pasek M, Cutler C, Koreth J, Alyea E, Sarantopoulos S, Antin JH, Ritz J, Canning C, Kutok J, Mihm MC, Dranoff G, Soiffer R
Proc. Natl. Acad. Sci. U.S.A., 2009-08-26;106(37):15825-30.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA (Capture)
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