Recombinant Human ULBP-5 Protein, CF

Catalog #: 7149-UL Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
7149-UL-050

R&D Systems Recombinant Proteins and Enzymes

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Recombinant Human ULBP-5 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human (rh) NKG2D Fc Chimera (Catalog # 1299-NK) is immobilized at 1 μg/mL (100 μL/well), the concentration of rhULBP-5 that produces 50% of the optimal binding response is found to be approximately 1.50-7.50 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human ULBP-5 protein
Met1-Gly210, with a C-terminal 6-His tag

Accession #

NP_001001788

N-terminal Sequence
Analysis

Asp29

Predicted Molecular Mass

21.5 kDa

SDS-PAGE

24-28 kDa, reducing conditions

Product Datasheets

7149-UL

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7149-UL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: ULBP-5

ULBP‑5 (cytomegalovirus glycoprotein UL16 binding protein 5), also called RAET1G (retinoic acid early transcript 1G), is a 37 kDa member of a family of cell‑surface proteins that function as ligands for human NKG2D (1‑3). When engaged, NKG2D activates cytolytic activity and/or cytokine production by effector cells that express it, such as NK cells, NKT cells, gamma δ T cells, and CD8⁺ alpha beta T cells (3‑5). Human ULBP‑5 mRNA encodes a 25 amino acid (aa) signal sequence, a 198 aa extracellular domain (ECD), a 20 aa transmembrane domain, and a 91 aa cytoplasmic sequence. Transmembrane, predicted soluble (RAET1G2), and glycosylphosphatidylinositol (GPI)‑anchored isoforms are identical through aa 210, while a shorter soluble form (RAET1G3) lacks aa 118‑154 (3‑5). Secreted, cell surface and intracellular ULBP‑5 have been described (3‑8). While most family members are GPI‑anchored, only ULBP‑5 and ULBP‑4/RAET1E express a transmembrane form (4, 5). Within the ECD, ULBP‑5 possesses two MHC Class I alpha domains and shares ~93% aa sequence identity with ULBP‑2/RAET1H plus ULBP‑6/RAET1L, and 30‑60% aa identity with remaining family members (1, 5). Rodent NKG2D ligands Rae‑1 alpha ‑ epsilon are, like ULBP proteins, distantly related to MHC class I proteins, but ULBP and Rae‑1 family proteins do not share significant sequence identity (2). ULBP‑5 mRNA expression is low or undetected in many tissues, but increased expression is reported in epithelial tissues and human decidua (5‑8). ULBP‑5 is expressed on some breast, epithelial and hematopoietic tumor cells and has been implicated in tumor surveillance (4‑6).

References