Recombinant Human ULBP-6/RAET1L Protein, CF

Catalog #: 7485-UL Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
7485-UL-050

R&D Systems Recombinant Proteins and Enzymes

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Recombinant Human ULBP-6/RAET1L Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human NKG2D/CD314 Fc Chimera (Catalog # 1299‑NK) is immobilized at 1 μg/mL (100 μL/well), the concentration of Recombinant Human ULBP-6/RAET1L that produces 50% of the optimal binding response is found to be approximately 0.8‑4 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human ULBP-6/RAET1L protein
Met1-Gly218, with a C-terminal 6-His tag

Accession #

AAK91503

N-terminal Sequence
Analysis

Arg26

Predicted Molecular Mass

22.6 kDa

SDS-PAGE

30-37 kDa, reducing conditions

Product Datasheets

7485-UL

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7485-UL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: ULBP-6/RAET1L

RAET1L (retinoic acid early transcript 1L), also called ULBP‑6 (cytomegalovirus glycoprotein UL16 binding protein 6), is an approximately 31 kDa glycophosphatidylinositol (GPI)‑linked member of a family of human cell‑surface proteins that are ligands for human NKG2D (1‑3). When engaged on effector cells that express it, such as NK cells and CD8⁺ T cells, NKG2D cooperates with other cell surface receptors to activate cytolytic activity and/or cytokine production (3). Human RAET1L mRNA encodes a 25 amino acid (aa) signal sequence, a 193 aa extracellular domain, and a 28 aa C‑terminal propeptide that is removed during GPI linkage. Single nucleotide polymorphisms of RAET1L that potentially affect its function have been described (3). Mature RAET1L shares high aa sequence identity with extracellular regions of ULBP‑2/RAET1H (97%) and ULBP‑5/RAET1G (92%), and 35‑60% aa identity with remaining family members (1, 3). Most family members, like ULBP‑6, are GPI anchored membrane proteins, although ULBP‑5/RAET1G and ULBP4/RAET1E express a transmembrane form (1‑3). Rodent NKG2D ligands Rae‑1 alpha ‑ epsilon are, like ULBP proteins, distantly related to MHC class I proteins, but ULBP and Rae‑1 family proteins do not share significant sequence identity (2). RAET1L mRNA shows restricted expression in the trachea and some HPV‑positive cervical carcinoma and colorectal carcinoma cell lines (3). It is induced upon cytomegalovirus (CMV) infection in primary human fibroblasts but, like several other CMV‑induced family members, is sequestered from the cell surface by UL16 (3). RAET1L shows stronger binding to NKG2D molecules and UL16 than ULBP‑5/RAET1G (3, 4).

References