Recombinant Human VIGR/GPR126 Fc Chimera Protein, CF

Catalog #: 10577-GP Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10577-GP-050

Recombinant Human VIGR/GPR126 Fc Chimera Protein Binding Activity.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human VIGR/GPR126 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Laminin is immobilized at 5 µg/mL (100 µL/well), Recombinant Human VIGR/GPR126 Fc Chimera (Catalog # 10577-GP) binds with an ED₅₀ of 1.5-12 µg/mL.

Source

Human embryonic kidney cell, HEK293-derived human VIGR/GPR126 protein

Human VIGF/GPR126 (Cys38-Lys437) Accession # AAH75798.1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

AAH75798.1

N-terminal Sequence
Analysis

Cys38

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

71 kDa

SDS-PAGE

96-111 kDa, under reducing conditions

Product Datasheets

10577-GP

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10577-GP

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Laminin is immobilized at 5 µg/mL (100 µL/well), Recombinant Human VIGR/GPR126 Fc Chimera (Catalog # 10577-GP) binds with an ED₅₀ of 1.5-12 µg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human VIGR/GPR126 Fc Chimera Protein (Catalog # 10577-GP) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 96-111 kDa and 180-210 kDa, respectively.

Reconstitution Calculator

Background: VIGR/GPR126

VIGR (Vascular Inducible G Protein-coupled Receptor), also known as ADGRG6, DREG, and GPR126, is a neuronal 7 TM pass (G protein)-coupled receptor (GPCR) involved in myelination and glial and Schwann cell development (1, 2). Human VIGR cDNA encodes a 1221 amino acid (aa) residue membrane protein with a 37 aa signal peptide, a 825 aa extracellular domain (ECD) with 27 potential N-linked glycosylation sites, seven transmembrane segments that span between aa 863 and aa 1113, and a 108 aa residue cytoplasmic domain. Within ECD human VIGR shares 83% aa sequence identity with mouse and rat VIGR. VIGR is essential for the development of diverse organs (1, 2). Type IV collagen, a major constituent of the basement membrane, binds to VIGR and activates its signaling function (3). This interaction stimulated the production of cAMP in rodent Schwann cells, which require VIGR activity to differentiate, and in human embryonic kidney (HEK293) cells expressing exogenous VIGR. Laminin-211 binds a novel laminin-binding domain in VIGR N-terminal fragment between aa 446 and 807 (4). VIGR-Laminin-211 interactions regulate terminal differentiation and myelination by ensuring appropriate levels of cAMP for a given stage of Schwann cell development (4).

References

Rughetti, A. et al. (2005) J. Immunol. 174:7764.
Engelstaedter, V. et al. (2012) BMC Cancer 12:600.
Taylor-Papadimitriou, J. et al. (1999) Biochim. Biophys. Acta 1455:301.
Geng, Y. et al. (2012) Front Oncol. 2:76.
Tanida, S. et al. (2013) J Biol Chem. 288:31842.
Beatson, R. et al. (2016) Nat Immunol. 17:1273.
Piyush, T. et al. (2017) Cell Death Differ. 24:1937.

Long Name

Vascular-inducible G Protein-coupled Receptor

Entrez Gene IDs

57211 (Human); 215798 (Mouse); 308376 (Rat)

Alternate Names

APG1; Developmentally regulated G-protein-coupled receptor; DREG; FLJ14937; G protein-coupled receptor 126; Gm222; GPR126; G-protein coupled receptor 126; HBV PreS1-transactivated protein 2; PS1TP2; Vascular inducible G protein-coupled receptor; vascular-inducible G protein-coupled receptor; VIGR; VIGRPS1TP2