Recombinant Human VSIG2 Fc Chimera Protein, CF

Catalog #: 9815-VS Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9815-VS-100

Recombinant Human VSIG2 Fc Chimera Protein SDS-PAGE

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human VSIG2 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IFN-gamma secretion by human peripheral blood mononuclear cells (PBMC). The ED₅₀ for this effect is 5-25 μg/mL.

Source

Mouse myeloma cell line, NS0-derived human VSIG2 protein

Human VSIG2 (Val24-Ala243) Accession # Q96IQ7-1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

Q96IQ7-1

N-terminal Sequence
Analysis

Val24

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

50 kDa

SDS-PAGE

54-65 kDa, reducing conditions

Product Datasheets

9815-VS

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9815-VS

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	12 months from date of receipt, ≤ -20 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

SDS-PAGE

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2 μg/lane of Recombinant Human VSIG2 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 54-65 kDa and 100-130 kDa, respectively.

Reconstitution Calculator

Background: VSIG2

V-set and immunoglobulin domain-containing protein 2 (VSIG2), also known as cortical thymocyte-like protein (CT-like protein), is a member of the immunoglobulin superfamily (Igsf) that was originally described in Xenopus and was referred to as cortical thymocyte marker in Xenopus, or CTX. The clone isolated from the human genomic library was called CTH (1). Mature human VSIG2 is a single-pass type I transmembrane protein with a 220 amino acid (aa) extracellular domain (ECD) that contains one C2-type and one V-type immunoglobulin-like domains, and a 63 aa cytoplasmic region linked to the ECD by a short 21 aa transmembrane domain. Human VSIG2 ECD shares 85% and 86% aa sequence identity with mouse and rat VSIG2, respectively. VSIG2 is structurally related to the B7 family of immune regulatory proteins. Our studies at R&D Systems show that VSIG2 inhibits T cell activation, including IL17 and interferon gamma production.

References

Chrétien, I. et al. (1998) Eur. J. Immunol. 28:4094.

Long Name

V-Set and Immunoglobulin Domain Containing 2

Entrez Gene IDs

23584 (Human); 57276 (Mouse); 300520 (Rat)

Alternate Names

2210413P10Rik; Cortical thymocyte-like protein; CTH; CTHCTXLcortical thymocyte receptor (X. laevis CTX) like; CT-like protein; CTXL; V-set and immunoglobulin domain containing 2; V-set and immunoglobulin domain-containing protein 2; VSIG2