Recombinant Human VSIG8 Fc Chimera Biotinylated Protein, CF

Name: Recombinant Human VSIG8 Fc Chimera Biotinylated Protein, CF
Brand: R&D Systems
SKU: BT9200
Price: 519 USD

Catalog #: BT9200 Datasheet / COA / SDS

Discontinued Product

BT9200 has been discontinued.

View all VSIG8 products.

Recombinant Human VSIG8 Fc Chimera Biotinylated Protein Bioactivity

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All VSIG8 Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human VSIG8 Fc Chimera Biotinylated Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED₅₀ for this effect is 1-5 μg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human VSIG8 protein

Human VSIG8 (Val22-Gly263) Accession # Q5VU13	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

N-terminal Sequence
Analysis

Val22

Structure / Form

Disulfide-linked homodimer. Biotinylated via sugars

Predicted Molecular Mass

54 kDa (unlabeled)

SDS-PAGE

56-67 kDa, reducing conditions

Product Datasheets

BT9200

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

BT9200

Formulation	Lyophilized from a 0.2 μm filtered solution in Sodium Acetate and Mannitol with Trehalose.
Reconstitution	Reconstitute at 200 μg/mL in water.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Both Biotinylated Recombinant Human VSIG8 Fc Chimera (Catalog # BT9200) and Recombinant Human VSIG8 Fc Chimera (Catalog # 9200-VS) inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED₅₀ for this effect is 1-5 µg/mL. The similarity in activity highlights that the biotinylated protein is fully functional.

Reconstitution Calculator

Background: VSIG8

VSIG8 (V-set and immunoglobulin domain containing 8), also known as C1orf204, is an approximately 45 kDa type I transmembrane protein of the B7 family within the Ig superfamily. Mature human VSIG8 consists of a 242 amino acid (aa) extracellular domain (ECD) containing two V-type Ig-like domains, a 21 aa transmembrane domain, and a 130 aa cytoplasmic domain. Within the ECD, human VSIG8 shares 88% and 89% aa identity with mouse and rat VSIG8, respectively. Alternative splicing generates a long isoform of human VSIG8 with a substitution in the cytoplasmic juxtamembrane region and a 124 aa extension at the C-terminus. VSIG8 was identified from proteomic analysis of human hair shafts (1, 2). It is expressed in the hair follicle and shaft, superficial layers of the nail matrix, and superficial layers of oral epithelium (3). R&D Systems in-house testing indicates that VSIG8 inhibits the production of IL-2 by activated T cells.

References