Recombinant Human VSTM2B Fc Chimera Avi-tag Protein, CF

Catalog #: AVI10060 Datasheet / COA / SDS

Biotinylated

Catalog #	Availability	Size / Price	Qty
AVI10060-050

Recombinant Human VSTM2B Fc Chimera Avi-tag Protein Binding Activity

2 Images

Product Details

Supplemental Products

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human VSTM2B Fc Chimera Avi-tag Protein, CF Summary

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells. The ED₅₀ for this effect is 1- 7.5 μg/mL Measured by its binding ability in a functional ELISA. When Goat Anti-Human IgG Fc Antibody (Catalog # G-102-C) is immobilized at 0.5 μg/mL (100 µL/well), the concentration of Biotinylated Recombinant Human VSTM2B Fc Chimera Avi-tag (Catalog # AVI10060) that produces 50% of the optimal binding response is found to be approximately 1-10 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human VSTM2B protein

Human VSTM2B (Ala29-Thr263) Accession # A6NLU5.2	IEGRMD	Human IgG₁ (Pro100-Lys330)	Avi-tag
N-terminus			C-terminus

Accession #

A6NLU5.2

N-terminal Sequence
Analysis

Ala29

Structure / Form

Disulfide-linked homodimer, biotinylated via Avi-tag

Predicted Molecular Mass

53 kDa

SDS-PAGE

60-72 kDa, under reducing conditions

Product Datasheets

AVI10060

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI10060

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

View Larger

When Goat Anti-Human IgG Fc Antibody (G-102-C) is immobilized at 0.5 μg/mL (100 µL/well), the concentration of Biotinylated Recombinant Human VSTM2B Fc Chimera Avi-tag (Catalog # AVI10060) that produces 50% of the optimal binding response is found to be approximately 1-10 ng/mL.

SDS-PAGE

View Larger

2 μg/lane of Biotinylated Recombinant Human VSTM2B Fc Chimera Avi-tag (Catalog # AVI10060) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 60-72 kDa and 120-144 kDa.

Reconstitution Calculator

Background: VSTM2B

V-set and transmembrane domain-containing protein 2B (VSTM2B) is a type I transmembrane protein belonging to the immunoglobulin (Ig) superfamily. There have been 8 VSTM family members identified and several have been shown to exhibit immunosuppressive activity (1). Human VSTM2B consists of an extracellular domain (ECD) with an Ig-like V-type domain, a transmembrane domain, and single amino acid (aa) in the cytoplasm. The mature ECD of human VSTM2B shares 86% and 85% aa sequence identity with mouse and rat VSTM2B, respectively. Currently, both the expression pattern and biological functions of VSTM2B remain unknown. Our in-house data show that VSTM2B inhibits the human T cell activation, including anti-CD3 induced IL-2, IFN-gamma secretion, and T cell proliferation. Our Avi-tag Biotinylated Recombinant VSTM2B features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

Long Name

V-Set And Transmembrane Domain Containing 2B

Entrez Gene IDs

342865 (Human); 58188 (Mouse); 361560 (Rat)

Alternate Names

V-set and transmembrane domain-containing protein 2B; VSTM2B