Recombinant Human VSTM2B Fc Chimera Protein, CF

Catalog #: 10060-VT Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10060-VT-050

Recombinant Human VSTM2B Fc Chimera Protein Bioactivity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human VSTM2B Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IFN-gamma secretion by human peripheral blood mononuclear cells (PBMC). The ED₅₀ for this effect is 1.5-7.5 μg/mL.

Source

Human embryonic kidney cell, HEK293-derived human VSTM2B protein

Human VSTM2B (Ala29-Thr263) Accession # A6NLU5	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

A6NLU5

N-terminal Sequence
Analysis

Ala29

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

51 kDa

SDS-PAGE

62-70 kDa, reducing conditions

Product Datasheets

10060-VT

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10060-VT

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Human VSTM2B Fc Chimera (Catalog # 100060-VT) inhibits IFN-gamma secretion by human peripheral blood mononuclear cells in the presence of anti-CD3 antibody. The ED₅₀ for this effect is 1.5-7.5 μg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human VSTM2B Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 62-70 kDa and 120 - 140 kDa, respectively.

Reconstitution Calculator

Background: VSTM2B

VSTM2B, or V-set and transmembrane domain-containing protein 2B is a membrane protein which includes a 235 amino acid (aa) extracellular domain that contains an Ig-like V-type domain, a 21 aa transmembrane domain, and one aa in cytoplasm. This extracellular domain shares 45% aa identity human VSTM2A. The mature extracellular domain of human VSTM2B shares 86% and 85% aa sequence identity for the mouse and rat homolog, respectively. Currently, both the expression pattern and biological functions of VSTM2B remain unknown. our in house data show that VSTM2B inhibits the human T cell activation, including anti-CD3 induced IL-2, IFN-gamma secretion, and T cell proliferation.

Long Name

V-Set And Transmembrane Domain Containing 2B

Entrez Gene IDs

342865 (Human); 58188 (Mouse); 361560 (Rat)

Alternate Names

V-set and transmembrane domain-containing protein 2B; VSTM2B