Is the 10 His-tag on Recombinant Human WISP-1/CCN4 Protein, CF, on the N-terminus or C-terminus?

Name: Recombinant Human WISP-1/CCN4 Protein, CF
Brand: R&D Systems
SKU: 1627-WS
Rating: 5 (1 reviews)

The 10 His-tag on Recombinant Human WISP-1/CCN4 Protein, CF, is on the C-terminus.

Recombinant Human WISP-1/CCN4 Protein, CF

Catalog #: 1627-WS
7 Citations 1 Review Datasheet / COA / SDS

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1627-WS-050

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Citations (7)

FAQs

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Reviews (1)

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human WISP-1/CCN4 Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit the cell growth of DU145 human prostate carcinoma cells. The ED₅₀ for this effect is <6.00 µg/mL.

Source

Mouse myeloma cell line, NS0-derived human WISP-1/CCN4 protein
Thr23-Asn367, with a 10-His tag

Accession #

O95388

N-terminal Sequence
Analysis

Thr23

Predicted Molecular Mass

39.3 kDa

SDS-PAGE

62 kDa, reducing conditions

Product Datasheets

1627-WS

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1627-WS

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 250 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: WISP-1/CCN4

Human WISP-1 (Wnt-induced secreted protein-1; also CNN4) is a 40 kDa, secreted, heparin-binding glycoprotein that is a member of the CCN (or CTGF/Cyr61/Nov) cysteine-rich protein family (1-5). It is synthesized as a 367 aa precursor that contains a series of structural homology modules. Following a 22 amino acid (aa) signal sequence, there is a 68 aa IGFBP-like domain (aa 53-120), a 57 aa von Willebrand factor type C (VWC) module (aa 126-182), a 40 aa TSP type I domain (aa 220-259) and a 75 aa, C-terminal cysteine knot motif (aa 273-347). The VWC module is associated with protein-protein interaction, the TSP domain binds sulfated glycoconjugates, and the cysteine knot mediates dimerization and receptor binding (4). It is likely that WISP-1 normally circulates as an 80 kDa homodimer (2). At least five alternative splice forms are known for WISP-1. One is 30 kDa in size, 258 aa in length, and shows a substitution of a His for aa 95-182. This removes the VWC domain (2, 6). A second isoform is 155 aa in length and shows a frameshift at Arg 117 with a unique 38 aa C-terminal extension. A third is 195 aa in length and shows a 31 aa substitution for the first 203 aa of the full length precursor (6). This retains the VWC and cysteine knot domains. A fourth shows a 43 aa substitution for aa 117-367 for a total length of 163 aa. This effectively removes everything but the IGFBP-like domain (7). The last splice form contains a deletion of aa 25-269 for a total length of 122 aa. Thus, only the signal sequence and cysteine knot motifs are retained (8). This leaves only the IGFBP-like domain (9). Full-length mature human WISP-1 is 85% aa identical to both mouse and rat WISP-1. WISP-1 is expressed by osteoblasts and may contribute to fracture healing by promoting bone cell formation (10, 11).

References

Pennica, D. et al. (1998) Proc. Natl. Acad. Sci. USA 95:14717.
Tanada, S. et al. (2001) Oncogene 20:5525.
Brigstock, D.R. et al. (2003) J. Clin. Pathol. Mol. Pathol. 56:127.
Desnoyers, L. (2004) Curr. Pharm. Des. 10:3913.
Brigstock, D.R. (2003) J. Endocrinol. 178:169.
Li, Z. (2005) GenBank Accession # AAP43925.
Li, Z. (2005) GenBank Accession # AAP43926.
Li, Z. (2005) GenBank Accession # AAP43924.
Cervello, M. et al. (2004) Ann. N.Y. Acad. Sci. 1028:432.
French, D.M. et al. (2004), Am. J. Pathol. 165:855.
Parisi, M.S. et al. (2006) Bone 38:671.

Long Name

Wnt-1 Induced Secreted Protein 1

Entrez Gene IDs

8840 (Human); 22402 (Mouse); 102125044 (Cynomolgus Monkey)

Alternate Names

CCN family member 4; CCN4; CCN4WISP1tc; WISP1; WISP-1; WISP1c; WISP1i; WNT1 induced secreted protein 1; WNT1 inducible signaling pathway protein 1; Wnt-1 inducible signaling pathway protein 1; wnt-1 signaling pathway protein 1; Wnt-1-induced secreted protein; WNT1-inducible-signaling pathway protein 1

Related Research Areas

Citations for Recombinant Human WISP-1/CCN4 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Wisp1 is a circulating factor that stimulates proliferation of adult mouse and human beta cells
Authors: R Fernandez-, A García-Ala, Y Esteban, J Mir-Coll, B Serra-Nava, M Fontcubert, C Broca, M Armanet, A Wojtuscisz, V Kram, MF Young, J Vidal, R Gomis, R Gasa
Nat Commun, 2020-11-25;11(1):5982.
Species: Human
Sample Types: Whole Tissue
Applications: Bioassay
Angiokine Wisp-1 is increased in myocardial infarction and regulates cardiac endothelial signaling
Authors: LH Wright, DJ Herr, SS Brown, H Kasiganesa, DR Menick
JCI Insight, 2018-02-22;3(4):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
BMP3 expression by osteoblast lineage cells is regulated by canonical Wnt signaling
Authors: S Kokabu, V Rosen
FEBS Open Bio, 2017-12-16;8(2):168-176.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
Differential expression of cell cycle and WNT pathway-related genes accounts for differences in the growth and differentiation potential of Wharton's jelly and bone marrow-derived mesenchymal stem cells
Authors: AK Batsali, C Pontikoglo, D Koutroulak, KI Pavlaki, A Damianaki, I Mavroudi, K Alpantaki, E Kouvidi, G Kontakis, HA Papadaki
Stem Cell Res Ther, 2017-04-26;8(1):102.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
WISP-1 overexpression upregulates cell proliferation in human salivary gland carcinomas via regulating MMP-2 expression
Onco Targets Ther, 2016-10-21;9(0):6539-6548.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Inhibition of fibroblast growth by Notch1 signaling is mediated by induction of Wnt11-dependent WISP-1.
Authors: Liu ZJ, Li Y, Tan Y
PLoS ONE, 2012-06-08;7(6):e38811.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Activation of Notch1 signaling in stromal fibroblasts inhibits melanoma growth by upregulating WISP-1.
Authors: Shao H, Cai L, Grichnik JM
Oncogene, 2011-04-25;30(42):4316-26.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

FAQs

Is the 10 His-tag on Recombinant Human WISP-1/CCN4 Protein, CF, on the N-terminus or C-terminus?
- The 10 His-tag on Recombinant Human WISP-1/CCN4 Protein, CF, is on the C-terminus.

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