Recombinant Mouse Ameloblastin Protein, CF

Catalog #: 3026-AB Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
3026-AB-050

R&D Systems Recombinant Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Ameloblastin Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED₅₀ for this effect is 1-4 μg/mL.

Source

Mouse myeloma cell line, NS0-derived mouse Ameloblastin protein
Val27-Pro422, with a C-terminal 6-His tag

Accession #

AAH87927

N-terminal Sequence
Analysis

Val27 & Ala29

Predicted Molecular Mass

43 kDa

SDS-PAGE

70-80 kDa, reducing conditions

Product Datasheets

3026-AB

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3026-AB

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Ameloblastin

Ameloblastin (AMBN) is a secreted bone and tooth-specific glycoprotein member of the enamel matrix protein family. Mouse Ameloblastin is synthesized by ameloblasts and osteoblasts as a 407 amino acid (aa; short or “a” isoform) or 422 aa (long or “b” isoform) protein with predicted molecular masses of 44 kDa or 45 kDa, respectively (1). The long isoform includes an additional 15 aa insert following aa 103 and contains additional glycosylation sites (2). This recombinant protein corresponds to the mature long isoform of mouse Ameloblastin. Ameloblastin may be cleaved following secretion leading to the generation of multiple sized proteins from 17-66 kDa (3-6). Mouse Ameloblastin (long isoform) shares 60% and 96% aa sequence identity with the human and rat orthologs, respectively. Ameloblastin is a critical component of tooth enamel and bone matrix where it binds calcium, thereby facilitating bone and tooth mineralization. It has also been shown to self-associate into ribbon-like supramolecular structures (7). During tooth development, it acts by binding to and maintaining the differentiated phenotype of secretory ameloblasts (8, 9). During bone development, Ameloblastin regulates osteogenic differentiation by binding to CD63 and promoting CD63-Integrin beta 1 interactions (10). Ameloblastin may also promote osteoclastogenesis (11). Ameloblastin can act as an adhesion molecule and promotes the adhesion of periodontal ligament cells via RhoA activation and ameloblasts via heparin-binding domains (8, 12, 13).

References