Recombinant Mouse Aminopeptidase PILS/ARTS1 Protein, CF

Name: Recombinant Mouse Aminopeptidase PILS/ARTS1 Protein, CF
Brand: R&D Systems
SKU: 2500-ZN
Rating: 5 (1 reviews)

Catalog #: 2500-ZN
1 Citation 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
2500-ZN-010

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Aminopeptidase PILS/ARTS1 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to cleave the fluorogenic peptide substrate, Leu-AMC. The specific activity is >100 pmol/min/µg, as measured under the described conditions.

Source

Mouse myeloma cell line, NS0-derived mouse Aminopeptidase PILS/ARTS1 protein
Ser27-Leu930, with a C-terminal 10-His tag

Accession #

Q6GTP5

N-terminal Sequence
Analysis

Ser27

Predicted Molecular Mass

105 kDa

SDS-PAGE

111 kDa, reducing conditions

Product Datasheets

2500-ZN

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2500-ZN

Formulation	Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

Assay Procedure

Materials

Assay Buffer: 25 mM Tris, pH 8.0
Recombinant Mouse Aminopeptidase PILS/ARTS1 (rmARTS1) (Catalog # 2500-ZN)
Substrate: Leu-AMC (Bachem, Catalog # I-1240)
F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent

Dilute rmARTS1 to 2 µg/mL in Assay Buffer.
Dilute Substrate to 200 µM in Assay Buffer.
Load 50 µL of the 2 µg/mL rmARTS1 into a plate, and start the reaction by adding 50 µL of 200 µM Substrate. Include a Substrate Blank containing 50 µL Assay Buffer and 50 µL of 200 µM Substrate.
Read at excitation and emission wavelengths of 380 nm and 460 nm, respectively, in kinetic mode for 5 minutes.
Calculate specific activity:

Specific Activity (pmol/min/µg) =	Adjusted V_max* (RFU/min) x Conversion Factor** (pmol/RFU)
	amount of enzyme (µg)

*Adjusted for Substrate Blank
**Derived using calibration standard 7-amino, 4-Methyl Coumarin AMC (Sigma, Catalog # A-9891).

Per Well:

rmARTS1: 0.1 µg
Substrate: 100 µM

Reconstitution Calculator

Background: Aminopeptidase PILS/ARTS1

The mouse ARTS1 gene encodes aminopeptidase PILS (Puromycin-Insensitive Leucyl-Specific), which is also known as adipocyte-derived leucine aminopeptidase, type 1 tumor necrosis factor receptor shedding aminopeptidase regulator and ERAAP (the aminopeptidase associated with antigen processing in the endoplasmic reticulum) (1-3). The deduced amino acid sequence of mouse ARTS1 consists of a signal peptide and a large ectodomain. Widely expressed and releasing an N‑terminal amino acid (Leu), the enzyme may play a role in many processes such as antigen processing and angiogenesis.

References

Miyashita, H. et al. (2002) Blood 99:3241.
Serwold, T. et al. (2002) Nature 419:480.
Schomburg, L. (2004) in Handbook of Proteolytic Enzymes. Barrett, et al. eds. p. 311, Academic Press, San Diego.

Long Name

Aminopeptidase Puromycin-insensitive Leucyl-specific

Entrez Gene IDs

51752 (Human); 80898 (Mouse); 102146570 (Cynomolgus Monkey)

Alternate Names

Adipocyte-derived leucine aminopeptidase; A-LAP; A-LAPALAP; Aminopeptidase PILS; ARTS1; ARTS1aminopeptidase regulator of TNFR1 shedding; ARTS-1PILSAP; EC 3.4.11; EC 3.4.11.-; EC 3.4.11.1; endoplasmic reticulum aminopeptidase 1; endoplasmic reticulum aminopeptidase associated with antigen processing; ERAAP1; ERAP1; KIAA0525APPILS; PILS-AP; PILS-APERAAP; Puromycin-insensitive leucyl-specific aminopeptidase; Type 1 tumor necrosis factor receptor shedding aminopeptidase regulator

Related Research Areas

Citation for Recombinant Mouse Aminopeptidase PILS/ARTS1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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