Recombinant Mouse ANGPTL8/Betatrophin Fc Chimera Protein, CF

Catalog #: 9983-AN Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9983-AN-050

Recombinant Mouse ANGPTL8/Betatrophin Fc Chimera Protein Binding Activity

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All Angiopoietin-like Protein 8/Betatrophin Proteins and Enzymes

Product Details

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse ANGPTL8/Betatrophin Fc Chimera Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse Angiopoietin-like 3 (Catalog # 9899-AN) is immobilized at 1 µg/mL (100 µL/well), Recombinant Mouse Angiopoietin-like Protein 8/Betatrophin Fc Chimera binds with an ED₅₀ of 0.12-0.72 μg/mL.

Source

Mouse myeloma cell line, NS0-derived mouse Angiopoietin-like Protein 8/Betatrophin protein

Mouse Betatrophin (Ala22-Ala198) Accession # Q8R1L8	IEGRMDP	Mouse IgG_2a (Glu98-Lys330)
N-terminus		C-terminus

Accession #

Q8R1L8

N-terminal Sequence
Analysis

Ala22

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

47 kDa

SDS-PAGE

52-56 kDa, reducing conditions

Product Datasheets

9983-AN

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9983-AN

Formulation	Lyophilized from a 0.2 μm filtered solution in MOPS,NaCl and CHAPS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in water.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Mouse Angiopoietin-like 3 (Catalog # 9899-AN) is immobilized at 1 µg/mL, Recombinant Mouse Betatrophin Fc Chimera (Catalog # 9983-AN) binds with an ED₅₀ of 0.12-0.72 µg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Mouse Angiopoietin-like Protein 8/Betatrophin was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 52-56 kDa and 105-110 kDa, respectively.

Reconstitution Calculator

Background: Angiopoietin-like Protein 8/Betatrophin

Betatrophin (also called ANGPTL8, lipasin and RIPL), a novel secretory protein from liver and fatty tissues, is believed to be involved in lipid and glucose metabolism. It is most homologous to ANGPTL3 but lacks the fibrinogen-like domain of ANGPTL3 and other ANGPTL family members (1). Mouse Betatrophin is 183 amino acids (aa) in length and shares 72% aa identity with human Betatrophin. Betatrophin is a crucial modulator in lipid metabolism. It can form a complex with the N-terminal of ANGPTL3, and the complex is necessary for inhibition of LPL and triglyceride modulation (2). Betatrophin did not control beta-cell expansion in the mouse model (3). In addition to the lipid and glucose metabolism, ANGPTL8 has been reported to be involved in many other disorders (4).

References

Quagliarini, F. et al. (2012) PNAS 109(48):19751.
Chi, X., et al. (2017) Molecular Metabolism 6:1137.
Cox A.R. et al. PLoS One (2016) 11:e0159276.
Luo, M. et al. (2018) Front Endocrino 9:169.

Entrez Gene IDs

55908 (Human); 624219 (Mouse); 100361444 (Rat)

Alternate Names

Angiopoietin like Protein 8; Angiopoietin-like Protein 8; ANGPTL8; Betatrophin; C19orf80; chromosome 19 open reading frame 80; Gm6484; hepatocellular carcinoma-associated gene TD26; hepatocellular carcinoma-associated protein TD26; Lipasin; PRO1185; PVPA599; RIFL; TD26; UNQ599