Recombinant Mouse ASGR2 Fc Chimera Protein, CF

Catalog #: 10447-AS Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10447-AS-050

Recombinant Mouse ASGR2 Fc Chimera Protein Bioactivity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse ASGR2 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse ASGR2 Fc Chimera (Catalog # 10447-AS) binds Human plasma von Willebrand Factor with an ED₅₀ of 0.05‑0.6 μg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived mouse ASGR2 protein

MDP	Mouse IgG_2A (Glu98-Lys330)	IEGR	Mouse ASGR2 (Ser79-His301) Accession # P24721.1
N-terminus			C-terminus

Accession #

P24721.1

N-terminal Sequence
Analysis

Met

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

53 kDa

SDS-PAGE

62-81 kDa, under reducing conditions

Product Datasheets

10447-AS

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10447-AS

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Immobilized Recombinant Mouse ASGR2 Fc Chimera (10447-AS) binds human plasma von Willebrand Factor with an ED₅₀of 0.05-0.6 μg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Mouse ASGR2 Fc Chimera (Catalog # 10447-AS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 62-81 kDa and 120-160 kDa, respectively.

Reconstitution Calculator

Background: ASGR2

Asialoglycoprotein receptor 2 (ASGR2), also known as hepatic lectin 2 (HL-2), is an approximately 38 kDa member of the C-type lectin receptor family (1). ASGR2 is a single-pass type II transmembrane protein that plays a role in the endocytosis of desialylated glycoproteins, hepatic thrombopoietin production, and gastrointestinal malignancies (2, 3). ASGR2 is closely related to ASGR1 which is about 2 kDa smaller and is encoded by distinct but closely linked genes. Variations in ASGR2 structure due to alternative splicing have been reported (4-6). Mature ASGR2 consists of a cytoplasmic domain, transmembrane segment and an extracellular domain (ECD). Within the ECD, mouse ASGR2 shares 67% and 83% aa sequence identity with human and rat ASGR2, respectively. Both ASGR2 and ASGR1 compose the asialoglycoprotein receptor (ASGPR) which mediates removal of potentially hazardous glycoconjugates from blood in health and disease (7). ASGPR, also known as the Ashwell receptor, can modulate von Willebrand factor (vWF) and platelet homeostasis in part via clearance of platelets that are first desialylated during sepsis caused by pathogens including S. pneumoniae. The ASGR1 chain of the Ashwell receptor participates in plasma vWF clearance independently of sialylation and sepsis, ASGR2 colocalization with plasma vWF was increased in ASGR1 deficient mice (8). In addition, ASGR2 expression was shown to be associated with malignant phenotypes in gastric cancer and may represent a specific biomarker for recurrence of some gastric cancers (9).

References

Ishibashi, S. et al. (1994) J. Biol. Chem. 269:27803.
Grozovsky, R. et al. (2015) Nat Med. 1:47.
Tanaka, H. et al. (2018) Mol. Cancer Res. 16:1420.
Drickamer, K. et al. (1984) J. Biol. Chem. 259:770.
Halberg, D.F. et al. (1987) J. Biol. Chem. 262:9828.
Paietta, E. et al. (1992) J. Biol. Chem. 267:11078.
Harris R.L. et al. (2012) Mol Biol Int. 2012:283974.
Grewal, P. et al. (2008) Nature Medicine. 14:6.
Tanaka H. et al. (2018) Mol Cancer Res. 16:1420.

Long Name

Asialoglycoprotein Receptor 2

Entrez Gene IDs

433 (Human); 11890 (Mouse); 29403 (Rat)

Alternate Names

ASGPR 2; ASGP-R 2; ASGPR2; ASGP-R2; ASGR2; asialoglycoprotein receptor 2; CLEC4H2; CLEC4H2FLJ60040; C-type lectin domain family 4 member H2; HBxAg-binding protein; HBXBP; Hepatic lectin H2; HL-2