Home / BAFF/BLyS/TNFSF13B / Recombinant Mouse BAFF/BLyS/TNFSF13B Protein

Recombinant Mouse BAFF/BLyS/TNFSF13B Protein

Catalog #: 8876-BF
35 Citations Datasheet / COA / SDS
Analyzed by SEC-MALS

Carrier Free

Catalog # Availability Size / Price Qty
8876-BF-010/CF

With Carrier

Catalog # Availability Size / Price Qty
8876-BF-010
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Recombinant Mouse BAFF/BLyS/TNFSF13B Protein SEC-MALS.
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Citations (35)
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Recombinant Mouse BAFF/BLyS/TNFSF13B Protein Summary

Product Specifications

Purity
>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using anti-IgM stimulated mouse B cells. The ED50 for this effect is 0.1‑0.5 ng/mL in the presence of goat anti‑mouse IgM µ chain.
Source
Mouse myeloma cell line, NS0-derived mouse BAFF/BLyS/TNFSF13B protein
Ala127-Leu309
Accession #
Q9WU72
N-terminal Sequence
Analysis
Ala127
Structure / Form
Noncovalently-linked homotrimer
Predicted Molecular Mass
21 kDa
SDS-PAGE
19-23 kDa (monomer) & 38-47 kDa (non-reducible dimer), reducing conditions

Product Datasheets

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8876-BF (with carrier)

Product Datasheet
COA
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8876-BF/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8876-BF

Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl and TCEP with BSA as a carrier protein..
Reconstitution Reconstitute at 100-500 μg/mL in sterile water.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

8876-BF/CF

Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl and TCEP.
Reconstitution Reconstitute at 100-500 μg/mL in sterile water.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

SEC-MALS View Larger

Recombinant Mouse BAFF/BLyS/TNFSF13B (Catalog # 8876-BF) has a molecular weight (MW) of 62.6 kDa as analyzed by SEC-MALS, suggesting that this protein is a homotrimer.  MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).

Bioactivity Recombinant Mouse BAFF/BLyS/TNFSF13B Protein Bioactivity View Larger

Recombinant Mouse BAFF/BLyS/TNFSF13B (Catalog # 8876-BF) induces mouse B cell proliferation. The ED50 for this effect is 0.1‑0.5 ng/mL.The ED50 for the two competitors is >5 ng/mL, which is more than 25-fold weaker.

Reconstitution Calculator

Reconstitution Calculator

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Background: BAFF/BLyS/TNFSF13B

B-cell activating factor (BAFF), also known as BLyS, TALL-1, THANK, and TNFSF13B, is a 32 kDa transmembrane glycoprotein in the TNF ligand superfamily. It is involved in multiple aspects of immune system regulation, particularly towards B cells (1, 2). Mature mouse BAFF consists of a 47 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 241 aa extracellular domain (ECD) with a stalk region and one TNF-like domain (3, 4). Within aa 127-309 of the ECD, mouse BAFF shares 72% aa sequence identity with human BAFF. It can be expressed as a homotrimer or as a heteromer in association with the related TNFSF member APRIL (4, 5). A 18 kDa fragment containing the TNF-like domain can be released by proteolysis between Arg126 and Ala127 (4). Soluble BAFF is stored intracellularly in neutrophils and released upon inflammatory stimulation (6). Alternative splicing generates an isoform termed deltaBAFF that lacks 19 aa between the proteolytic cleavage site and the TNF-like domain. deltaBAFF can form heteromers with BAFF and negatively regulates BAFF function (7). BAFF is produced by many hematopoietic cell types including by monocytes, macrophages, neutrophils, dendritic cells, and T cells and also by adipocytes (1, 2, 8). Both BAFF and APRIL are functional ligands for the TNF receptor superfamily members BCMA and TACI, and BAFF additionally binds and signals through BAFF R (9, 10). All three receptors are primarily expressed by B cells (10). BAFF plays a critical role in the development and survival of B lineage cells (2, 11, 12). Mice that over-express BAFF exhibit elevated B cell numbers, increased formation and size of germinal centers, and symptoms of autoimmunity (13). Soluble BAFF is elevated in B cell malignancies, autoimmunity, and other immune disorders (1). In addition, BAFF costimulates T cell activation, promotes a Th1 biased immune response, and promotes the expansion of Treg cells (14-16). BAFF also promotes monocyte survival, proinflammatory cytokine secretion, and differentiation to macrophages (17).

References
  1. Lied, G.A. and A. Berstad (2011) Scand. J. Immunol. 73:1.
  2. Mackay, F. et al. (2010) Immunol. Rev. 237:205.
  3. Moore, P.A. et al. (1999) Science 285:260.
  4. Schneider, P. et al. (1999) J. Exp. Med. 189:1747.
  5. Roschke, V. et al. (2002) J. Immunol. 169:4314.
  6. Scapini, P. et al. (2003) J. Exp. Med. 197:297.
  7. Gavin, A.L. et al. (2003) J. Biol. Chem. 278:38220.
  8. Alexaki, V.-I. et al. (2009) J. Immunol. 183:5948.
  9. Yu, G. et al. (2000) Nat. Immunol. 1:252.
  10. Thompson, J.S. et al. (2001) Science 293:2108.
  11. Schiemann, B. et al. (2001) Science 293:2111.
  12. Litinskiy, M.B. et al. (2002) Nat. Immunol. 3:822.
  13. Batten, M. et al. (2000) J. Exp. Med. 192:1453.
  14. Huard, B. et al. (2001) J. Immunol. 167:6225.
  15. Sutherland, A.P.R. et al. (2005) J. Immunol. 174:5537.
  16. Walters, S. et al. (2009) J. Immunol. 182:793.
  17. Chang, S.K. et al. (2006) Blood 108:2687.
Long Name
B cell Activating Factor
Entrez Gene IDs
10673 (Human); 24099 (Mouse); 498666 (Rat); 102123214 (Cynomolgus Monkey)
Alternate Names
ApoL related ligand TALL-1; B lymphocyte stimulator; BAFF; BAFFB-cell activating factor; B-cell-activating factor; BLyS; BLYSB-lymphocyte stimulator; CD257 antigen; CD257; Dendritic cell-derived TNF-like molecule; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; TNF- and APOL-related leukocyte expressed ligand 1; TNF and ApoL-related leukocyte expressed ligand 1; TNF homolog that activates apoptosis; TNFSF13B; TNFSF20; tumor necrosis factor (ligand) superfamily, member 13b; tumor necrosis factor (ligand) superfamily, member 20; tumor necrosis factor ligand superfamily member 13B; tumor necrosis factor superfamily, member 13b; tumor necrosis factor-like protein ZTNF4; ZTNF4

Citations for Recombinant Mouse BAFF/BLyS/TNFSF13B Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

35 Citations: Showing 1 - 10
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  1. IL-4 Licenses B Cell Activation Through Cholesterol Synthesis
    Authors: Steach, HR;York, AG;Skadow, MH;Chen, S;Zhao, J;Williams, KJ;Zhou, Q;Hsieh, WY;Brewer, JR;Qu, R;Shyer, JA;Harman, C;Sefik, E;Mowell, WK;Bailis, W;Cui, C;Kluger, Y;Bensinger, SJ;Craft, J;Flavell, RA;
    bioRxiv : the preprint server for biology
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. BAFF deficiency aggravated optic nerve crush-induced retinal ganglion cells damage by regulating apoptosis and neuroinflammation via NF-?B-I?B? signaling
    Authors: Lin, J;Deng, W;Liao, J;Ke, D;Cui, L;Zhong, H;Huang, K;Jiang, L;Chen, Q;Xu, F;Tang, F;
    International immunopharmacology
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. TRAF2/3 deficient B cells resist DNA damage-induced apoptosis via NF-?B2/XIAP/cIAP2 axis and IAP antagonist sensitizes mutant lymphomas to chemotherapeutic drugs
    Authors: Vashisht, M;Ge, H;John, J;McKelvey, HA;Chen, J;Chen, Z;Wang, JH;
    Cell death & disease
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Toll-like receptor mediated inflammation directs B cells towards protective antiviral extrafollicular responses
    Authors: Lam, JH;Baumgarth, N;
    Nature communications
    Species: Mouse, Transgenic Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Modelling Microglial Innate Immune Memory In Vitro: Understanding the Role of Aerobic Glycolysis in Innate Immune Memory
    Authors: Towriss, M;MacVicar, B;Ciernia, AV;
    International journal of molecular sciences
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. EDA2R-NIK signalling promotes muscle atrophy linked to cancer cachexia
    Authors: Bilgic, SN;Domaniku, A;Toledo, B;Agca, S;Weber, BZC;Arabaci, DH;Ozornek, Z;Lause, P;Thissen, JP;Loumaye, A;Kir, S;
    Nature
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. BAFF and APRIL counterregulate susceptibility to inflammation-induced preterm birth
    Authors: JR Doll, ME Moreno-Fer, TE Stankiewic, JL Wayland, A Wilburn, B Weinhaus, CA Chougnet, D Giordano, M Cappellett, P Presicce, SG Kallapur, N Salomonis, T Tilburgs, S Divanovic
    Cell Reports, 2023-04-05;42(4):112352.
    Species: Mouse
    Sample Types:
    Applications: In vivo assay
  8. BAFF induces CXCR5 expression during B cell differentiation in bone marrow
    Authors: H Koizumi, W Fujii, C Sanjoba, Y Goto
    Biochemistry and Biophysics Reports, 2023-03-07;34(0):101451.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Role of B Cell-Activating Factor in Fibrosis Progression in a Murine Model of Non-Alcoholic Steatohepatitis
    Authors: K Kanemitsu-, M Abe, Y Nakamura, T Miyake, T Watanabe, O Yoshida, Y Koizumi, M Hirooka, Y Tokumoto, B Matsuura, M Koizumi, Y Hiasa
    International Journal of Molecular Sciences, 2023-01-28;24(3):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Role of B Cell-Activating Factor in Fibrosis Progression in a Murine Model of Non-Alcoholic Steatohepatitis
    Authors: K Kanemitsu-, M Abe, Y Nakamura, T Miyake, T Watanabe, O Yoshida, Y Koizumi, M Hirooka, Y Tokumoto, B Matsuura, M Koizumi, Y Hiasa
    International Journal of Molecular Sciences, 2023;24(3):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Efficient human-like antibody repertoire and hybridoma production in trans-chromosomic mice carrying megabase-sized human immunoglobulin loci
    Authors: H Satofuka, S Abe, T Moriwaki, A Okada, K Kazuki, H Tanaka, K Yamazaki, G Hichiwa, K Morimoto, H Takayama, Y Nakayama, S Hatano, Y Yada, Y Murakami, Y Baba, M Oshimura, K Tomizuka, Y Kazuki
    Nature Communications, 2022-04-05;13(1):1841.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Circulating CD138 enhances disease progression by augmenting autoreactive antibody production in a mouse model of systemic lupus erythematosus
    Authors: L Liu, M Akkoyunlu
    The Journal of Biological Chemistry, 2021-08-06;0(0):101053.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Regulation of B cell functions by S-nitrosoglutathione in the EAE model
    Authors: J Kim, SMT Islam, F Qiao, AK Singh, M Khan, J Won, I Singh
    Redox Biology, 2021-06-23;45(0):102053.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  14. A BAFF/APRIL axis regulates obesogenic diet-driven weight gain
    Authors: CC Chan, ITW Harley, PT Pfluger, A Trompette, TE Stankiewic, JL Allen, ME Moreno-Fer, MSMA Damen, JR Oates, PC Alarcon, JR Doll, MJ Flick, LM Flick, J Sanchez-Gu, R Mukherjee, R Karns, M Helmrath, TH Inge, SP Weisberg, SJ Pamp, DA Relman, RJ Seeley, MH Tschöp, CL Karp, S Divanovic
    Nature Communications, 2021-05-18;12(1):2911.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  15. NR4A nuclear receptors restrain B cell responses to antigen when second signals are absent or limiting
    Authors: C Tan, R Hiwa, JL Mueller, V Vykunta, K Hibiya, M Noviski, J Huizar, JF Brooks, J Garcia, C Heyn, Z Li, A Marson, J Zikherman
    Nat. Immunol., 2020-08-31;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Etanercept Inhibits B Cell Differentiation by Regulating TNFRII/TRAF2/NF-kappaB Signaling Pathway in Rheumatoid Arthritis
    Authors: XY Cai, Y Zhu, C Wang, XY Tang, L Han, JL Shu, XZ Zhang, FQ Liang, JR Ge, L Xu, D Mei, LL Zhang, W Wei
    Front Pharmacol, 2020-05-12;11(0):676.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  17. BCR-Induced Ca2+ Signals Dynamically Tune Survival, Metabolic Reprogramming, and Proliferation of Naive B Cells
    Authors: CT Berry, X Liu, A Myles, S Nandi, YH Chen, U Hershberg, IE Brodsky, MP Cancro, CJ Lengner, MJ May, BD Freedman
    Cell Rep, 2020-04-14;31(2):107474.
    Species: Mouse
    Sample Types: Naive Splenic B-Cells
    Applications: Cell Culture
  18. E3 Ubiquitin Ligase Fbw7 Regulates the Survival of Mature B Cells
    Authors: P Ramezani-R, CR Leung, JR Apgar, RC Rickert
    J. Immunol., 2020-01-31;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  19. MicroRNA-29a Counteracts Glucocorticoid Induction of Bone Loss through Repressing TNFSF13b Modulation of Osteoclastogenesis
    Authors: RW Wu, WS Lian, YS Chen, CW Kuo, HC Ke, CK Hsieh, SY Wang, JY Ko, FS Wang
    Int J Mol Sci, 2019-10-17;20(20):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  20. Tumor-derived Thymic stromal lymphopoietin expands bone marrow B-cell precursors in circulation to support metastasis
    Authors: E Ragonnaud, K Moritoh, M Bodogai, F Gusev, S Garaud, C Chen, X Wang, T Baljinnyam, KG Becker, RW Maul, K Willard-Ga, E Rogaev, A Biragyn
    Cancer Res., 2019-10-01;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  21. Iron-dependent histone 3 lysine 9 demethylation controls B cell proliferation and humoral immune responses
    Authors: Y Jiang, C Li, Q Wu, P An, L Huang, J Wang, C Chen, X Chen, F Zhang, L Ma, S Liu, H He, S Xie, Y Sun, H Liu, Y Zhan, Y Tao, Z Liu, X Sun, Y Hu, Q Wang, D Ye, J Zhang, S Zou, Y Wang, G Wei, Y Liu, Y Shi, Y Eugene Chi, Y Hao, F Wang, X Zhang
    Nat Commun, 2019-07-03;10(1):2935.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  22. Arp2/3 complex-driven spatial patterning of the BCR enhances immune synapse formation, BCR signaling and B cell activation
    Authors: M Bolger-Mun, K Choi, JM Scurll, L Abraham, RS Chappell, D Sheen, M Dang-Lawso, X Wu, JJ Priatel, D Coombs, JA Hammer, MR Gold
    Elife, 2019-06-03;8(0):.
    Species: Complex Species Category
    Sample Types: Whole Cells
    Applications: Bioassay
  23. TACI expression and plasma cell differentiation are impaired in the absence of functional I?BNS
    Authors: S Khoenkhoen, E Erikson, M Ádori, JM Stark, JL Scholz, MP Cancro, GK Pedersen, GB Karlsson H
    Immunol. Cell Biol., 2019-01-30;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  24. Integrated B Cell, Toll-like, and BAFF Receptor Signals Promote Autoantibody Production by Transitional B Cells
    Authors: SW Du, HM Jacobs, T Arkatkar, DJ Rawlings, SW Jackson
    J. Immunol., 2018-10-29;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Stimulation
  25. Innate and adaptive signals enhance differentiation and expansion of dual-antibody autoreactive B cells in lupus
    Authors: A Sang, T Danhorn, JN Peterson, AL Rankin, BP O'Connor, SM Leach, RM Torres, R Pelanda
    Nat Commun, 2018-09-28;9(1):3973.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  26. BAFF inhibition attenuates fibrosis in scleroderma by modulating the regulatory and effector B cell balance
    Authors: T Matsushita, T Kobayashi, K Mizumaki, M Kano, T Sawada, M Tennichi, A Okamura, Y Hamaguchi, Y Iwakura, M Hasegawa, M Fujimoto, K Takehara
    Sci Adv, 2018-07-11;4(7):eaas9944.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  27. VprBP (DCAF1) Regulates RAG1 Expression Independently of Dicer by Mediating RAG1 Degradation
    Authors: NM Schabla, GA Perry, VL Palmer, PC Swanson
    J. Immunol., 2018-06-20;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  28. NF-?B inducing kinase is a therapeutic target for systemic lupus erythematosus
    Authors: HD Brightbill, E Suto, N Blaquiere, N Ramamoorth, S Sujatha-Bh, EB Gogol, GM Castanedo, BT Jackson, YC Kwon, S Haller, J Lesch, K Bents, C Everett, PB Kohli, S Linge, L Christian, K Barrett, A Jaochico, LM Berezhkovs, PW Fan, Z Modrusan, K Veliz, MJ Townsend, J DeVoss, AR Johnson, R Godemann, WP Lee, CD Austin, BS McKenzie, JA Hackney, JJ Crawford, ST Staben, MH Alaoui Ism, LC Wu, N Ghilardi
    Nat Commun, 2018-01-12;9(1):179.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  29. Regulation of type 1 diabetes development and B-cell activation in nonobese diabetic mice by early life exposure to a diabetogenic environment
    Authors: A De Riva, M Wållberg, F Ronchi, R Coulson, A Sage, L Thorne, I Goodfellow, KD McCoy, M Azuma, A Cooke, R Busch
    PLoS ONE, 2017-08-03;12(8):e0181964.
    Species: Mouse
    Sample Types:
    Applications: ELISA (Standard)
  30. PD-L2 Regulates B-1 Cell Antibody Production against Phosphorylcholine through an IL-5-Dependent Mechanism
    Authors: JT McKay, MA Haro, CA Daly, RD Yammani, B Pang, WE Swords, KM Haas
    J. Immunol., 2017-08-02;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  31. CD11b regulates antibody class switching via induction of AID
    Authors: S Park, H Sim, HI Kim, D Jeong, G Wu, SY Cho, YS Lee, HJ Kwon, K Lee
    Mol. Immunol., 2017-04-11;87(0):47-59.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  32. BCR and Endosomal TLR Signals Synergize to Increase AID Expression and Establish Central B Cell Tolerance
    Authors: M Kuraoka, PB Snowden, T Nojima, L Verkoczy, BF Haynes, D Kitamura, G Kelsoe
    Cell Rep, 2017-02-14;18(7):1627-1635.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  33. Gsk3 is a metabolic checkpoint regulator in B cells
    Authors: J Jellusova, MH Cato, JR Apgar, P Ramezani-R, CR Leung, C Chen, AD Richardson, EM Conner, RJ Benschop, JR Woodgett, RC Rickert
    Nat. Immunol, 2017-01-23;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  34. Differing Requirements for MALT1 Function in Peripheral B Cell Survival and Differentiation
    Authors: Peishan Lee
    J. Immunol, 2016-12-28;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  35. Impaired B cell receptor signaling is responsible for reduced TACI expression and function in X-linked immunodeficient mice.
    Authors: Uslu K, Coleman A, Allman W, Katsenelson N, Bram R, Alugupalli K, Akkoyunlu M
    J Immunol, 2014-03-19;192(8):3582-95.
    Species: Mouse, Transgenic Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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