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Recombinant Mouse beta IG-H3 Protein, CF

Catalog #: 2559-BG
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2559-BG-050
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Recombinant Mouse beta IG-H3 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. rm beta IG-H3, immobilized at 5 µg/mL (100 µL/well), can induce greater than 50% cell adhesion.
Source
Mouse myeloma cell line, NS0-derived mouse beta IG-H3 protein
Gly24-His683, with a C-terminal 6-His tag
Accession #
NP_033395
N-terminal Sequence
Analysis
Gly24
Predicted Molecular Mass
73.1 kDa
SDS-PAGE
70 kDa, reducing conditions

Product Datasheets

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2559-BG

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2559-BG

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: beta IG-H3

Beta IG-H3, also known as TGFBI and RGD-CAP, is a matricellular adaptor protein that is induced in most cell types in response to TGF-beta stimulation (1 - 4). The mouse beta IG-H3 cDNA encodes a 683 amino acid (aa) precursor that includes a 23 aa signal sequence, one EMI domain, four FAS1 domains, and one RGD motif (2). Mouse beta IG-H3 shares 91% aa sequence identity with human and porcine beta IG-H3. beta IG-H3 is expressed as a 75 kDa protein with no post-translational additions (5). Following secretion, cleavages at multiple positions near the C-terminal end liberate peptides with pro-apoptotic activity (5, 6). Peptides that encompass the RGD motif contribute to the pro-apoptotic effects of TGF-beta (6). FAS1 domains contain YH motifs that are characterized by conserved Tyr and His residues (7). The YH motifs in each of the FAS1 domains enable beta IG-H3 to bind to matrix fibronectin, collagen I, collagen VI, biglycan, and decorin (3, 8 - 11), in addition to cell expressed integrins alpha V/ beta 3, alpha V beta 5, and alpha 3 beta 1 (7, 8, 12, 13). The expression of beta IG-H3 is modulated at particular developmental stages in some cell types. It is upregulated in keratinocytes and immature dendritic cells but downregulated in osteoblasts (8, 12, 14). It promotes keratinocyte differentiation but blocks osteoblast differentiation (8, 12). beta IG-H3 stimulates macrophage endocytosis and vascular endothelial cell proliferation and migration (13, 14). High glucose levels induce beta IG-H3 in renal proximal tubule cells which is predictive of diabetic nephropathy (3). Several point mutations (clustered in the fourth FAS1 domain) of beta IG-H3 are linked to different corneal dystrophies (15). beta IG-H3 is downregulated in many cancers (4, 16) and functions as a suppressor of tumorigenicity when overexpressed (2, 4, 16).

References
  1. Skonier, J. et al. (1992) DNA Cell Biol. 11:511.
  2. Skonier, J. et al. (1994) DNA Cell Biol. 13:571.
  3. Lee, S-H. et al. (2003) Kidney Int. 64:1012.
  4. Zhao, Y.L. et al. (2002) Oncogene 21:7471.
  5. Andersen, R.B. et al. (2004) Biochemistry 43:16374.
  6. Kim, J-E. et al. (2003) Oncogene 22:2045.
  7. Kim, J-E. et al. (2002) J. Biol. Chem. 277:46159.
  8. Thapa, N. et al. (2005) Bone 36:232.
  9. Hanssen, E. et al. (2003) J. Biol. Chem. 278:24334.
  10. Billings, P.C. et al. (2002) J. Biol. Chem. 277:28003.
  11. Reinboth, B. et al. (2006) J. Biol. Chem. 281:7816.
  12. Oh, J-E. et al. (2005) J. Biol. Chem. 280:21629.
  13. Nam, J-O. et al. (2003) J. Biol. Chem. 278:25902.
  14. Cao, W. et al. (2006) Blood 107:2777.
  15. Stewart, H.S. et al. (1999) Hum. Mutat. 14:126.
  16. Zhao, Y. et al. (2006) Mol. Carcinog. 45:84.
Long Name
TGF-beta Induced Gene H3
Entrez Gene IDs
7045 (Human); 21810 (Mouse)
Alternate Names
beta IGH3; beta IG-H3; BIGH3EBMD; CDB1; CDG2; CDGG1transforming growth factor, beta-induced, 68kD; CSD; CSD1; CSD2; CSD3; Kerato-epithelin; LCD1; RGD-CAP; RGD-containing collagen-associated protein; TGFBI; TGFBIP; transforming growth factor, beta-induced, 68kDa; transforming growth factor-beta-induced protein ig-h3

Citation for Recombinant Mouse beta IG-H3 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. TGFBI deficiency predisposes mice to spontaneous tumor development.
    Authors: Zhang Y, Wen G, Shao G, Wang C, Lin C, Fang H, Balajee AS, Bhagat G, Hei TK, Zhao Y
    Cancer Res., 2009-01-01;69(1):37-44.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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