Recombinant Mouse BMP-6 Protein Summary
Product Specifications
Ser372-His510
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
6325-BM
| Formulation | Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 200 μg/mL in 4 mM HCl containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
6325-BM/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in HCl. |
| Reconstitution | Reconstitute at 200 μg/mL in 4mM HCl. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: BMP-6
Bone Morphogenetic Protein 6 (BMP‑6), also known as Vgr-1, is one of at least 15 structurally and functionally related BMPs which are members of the transforming growth factor beta (TGF-beta ) superfamily. Mouse BMP‑6 is synthesized as a 510 amino acid (aa) precursor protein that is cleaved at the dibasic cleavage site (RxxR) to release the 18 kDa C‑terminal mature protein. Biologically active BMP‑6 consists of a disulfide-linked homodimer of the mature proteins (1, 2). Mature mouse BMP‑6 shares 96% and 98% aa sequence identity with human and rat BMP‑6, respectively. Cellular responses to BMP‑6 are mediated by hetero-oligomeric complexes of type I (Activin RIA/ALK-2 and BMPR-IA/ALK-3) and type II (Activin RIIA and BMPR-II) serine/threonine kinase receptors (3 - 5). Glycosylation of BMP‑6 is required for its interaction with Activin RIA (6). BMP‑6 induces the expression of Noggin and is subsequently antagonized by Noggin (7, 8). BMP‑6 induces a wide range of cellular responses. It promotes osteoblast differentiation from mesenchymal stem cells (5), chondrocyte maturation (9), Ang II-induced aldosterone production in the adrenal cortex (3), hormone production and responsiveness in ovarian granulosa cells (10), iNOS and TNF-alpha production in macrophages (4), the cell death of B cells (8), and neurite outgrowth (11). BMP‑6 expression is induced in astrocytes surrounding sites of brain injury where it functions as a neuroprotectant (11, 12). BMP‑6 is upregulated during prostate cancer progression and promotes tumor cell metastasis to bone (13). Through interactions with the BMP coreceptor RGM‑C/Hemojuvelin, BMP‑6 plays an important role in iron homeostasis by promoting Hepcidin expression and preventing serum iron overload (14, 15).
- Chen, D. et al. (2004) Growth Factors 22:233.
- Lyons, K. et al. (1989) Proc. Natl. Acad. Sci. 86:4554.
- Inagaki, K. et al. (2006) Endocrinology 147:2681.
- Hong, J.H. et al. (2008) Immunology 128:e442.
- Lavery, K. et al. (2008) J. Biol. Chem. 283:20948.
- Saremba, S. et al. (2008) FEBS J. 275:172.
- Haudenschild, D.R. et al. (2004) Cancer Res. 64:8276.
- Kersten, C. et al. (2005) BMC Immunol. 6:9.
- Grimsrud, C.D. et al. (1999) J. Bone Miner. Res. 14:475.
- Shi, J. et al. (2009) Fertil. Steril. 92:1794.
- Yabe, T. et al. (2002) J. Neurosci. Res. 68:161.
- Zhang, Z. et al. (2006) Neuroscience 138:47.
- Dai, J. et al. (2005) Cancer Res. 65:8274.
- Meynard, D. et al. (2009) Nat. Genet. 41:478.
- Andriopoulos, B. Jr. et al. (2009) Nat. Genet. 41:482.
Citations for Recombinant Mouse BMP-6 Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Berberine and aspirin prevent traumatic heterotopic ossification by inhibition of BMP signalling pathway and osteogenic differentiation
Authors: Fan, J;Gao, J;Chen, J;Hou, J;Liu, M;Dang, Y;Lin, H;
Journal of cellular and molecular medicine
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Cardiac Hamp mRNA Is Predominantly Expressed in the Right Atrium and Does Not Respond to Iron
Authors: M Bigorra Mi, E Charlebois, S Tsyplenkov, C Fillebeen, K Pantopoulo
International Journal of Molecular Sciences, 2023-03-08;24(6):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Hematopoietic-Mesenchymal Signals Regulate the Properties of Mesenchymal Stem Cells
Authors: S Kanazawa, H Okada, D Riu, Y Mabuchi, C Akazawa, J Iwata, K Hoshi, A Hikita
International Journal of Molecular Sciences, 2022-07-26;23(15):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Factors affecting the induction of uncoupling protein 1 in C2C12 myogenic cells
Authors: T Yamamoto, Z Diao, M Murakami, F Shimokawa, T Matsui, M Funaba
Cytokine, 2022-06-20;157(0):155936.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Mesenchymal stromal cells in the bone marrow niche consist of multi-populations with distinct transcriptional and epigenetic properties
Authors: S Kanazawa, H Okada, H Hojo, S Ohba, J Iwata, M Komura, A Hikita, K Hoshi
Scientific Reports, 2021-08-04;11(1):15811.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
BMP signaling suppresses Gemc1 expression and ependymal differentiation of mouse telencephalic progenitors
Authors: H Omiya, S Yamaguchi, T Watanabe, T Kuniya, Y Harada, D Kawaguchi, Y Gotoh
Scientific Reports, 2021-01-12;11(1):613.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Metformin attenuates trauma-induced heterotopic ossification via inhibition of Bone Morphogenetic Protein signalling
Authors: H Lin, F Shi, S Jiang, Y Wang, J Zou, Y Ying, D Huang, L Luo, X Yan, Z Luo
J Cell Mol Med, 2020-11-10;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture -
Hepatocyte toll-like receptor 4 mediates lipopolysaccharide-induced hepcidin expression
Authors: YS Lee, YH Kim, YS Jung, KS Kim, DK Kim, SY Na, JM Lee, CH Lee, HS Choi
Exp. Mol. Med., 2017-12-08;49(12):e408.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Exosomes from mesenchymal stem cells induce the conversion of hepatocytes into progenitor oval cells
Authors: HH Wu, OK Lee
Stem Cell Res Ther, 2017-05-23;8(1):117.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
H3K9 methylation is a barrier during somatic cell reprogramming into iPSCs.
Authors: Chen, Jiekai, Liu, He, Liu, Jing, Qi, Jing, Wei, Bei, Yang, Jiaqi, Liang, Hanquan, Chen, You, Chen, Jing, Wu, Yaran, Guo, Lin, Zhu, Jieying, Zhao, Xiangjie, Peng, Tianran, Zhang, Yixin, Chen, Shen, Li, Xuejia, Li, Dongwei, Wang, Tao, Pei, Duanqing
Nat Genet, 2012-12-02;45(1):34-42.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
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Reason for Rating: Bio-activity test on HepG2 cells shows that this product has good cellular effects as published. See attached image file.
