Recombinant Mouse BTN2A2/Butyrophilin 2A2 Protein, CF

Catalog #: 8997-BT Datasheet / COA / SDS

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8997-BT-050

Recombinant Mouse BTN2A2/Butyrophilin 2A2 Protein Bioactivity

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All BTN2A2/Butyrophilin 2A2 Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse BTN2A2/Butyrophilin 2A2 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED₅₀ for this effect is 1-5 μg/mL.

Source

Mouse myeloma cell line, NS0-derived mouse BTN2A2/Butyrophilin 2A2 protein
Gln30-Trp248, with a C-terminal 6-His tag

Accession #

A4QPC6

N-terminal Sequence
Analysis

No results obtained. Gln30 inferred from enzymatic pyroglutamate treatment revealing Phe31

Predicted Molecular Mass

26 kDa

SDS-PAGE

38-45 kDa, reducing conditions

Product Datasheets

8997-BT

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8997-BT

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS and EDTA.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Mouse BTN2A2/Butyrophilin 2A2 inhibits IL-2 secretion by mouse T cells in the presence of anti-CD3 antibody. The ED₅₀ for this effect is 1-6 ug/mL.

Reconstitution Calculator

Background: BTN2A2/Butyrophilin 2A2

Butyrophilin 2A2 (BTN2A2) is a widely expressed 65 kDa type I transmembrane glycoprotein that functions as a negative regulator of immune responses (1). Mature mouse Butyrophilin 2A2 consisits of a 215 amino acid (aa) extracellular domain with two immunoglobulin-like domains, a 21 aa transmembrane segment, and a 249 aa cytoplasmic domain. Within the Ig-like domains, mouse Butyrophilin 2A2 shares 71% and 90% aa sequence identity with human and rat Butyrophilin 2A2, respectively. Alternative splicing generates an additional isoform of mouse Butyrophilin 2A2 with a deletion and substitution in the cytoplasmic region. Within the immune system, Butyrophilin 2A2 is expressed on thymic epithelial cells, naïve B cells, splenic NK cells, dendritic cells, and peritoneal macrophages and is up-regulated with cell activation (2, 3). Butyrophilin 2A2 inhibits T cell proliferation and activation and enhances the development of FoxP3⁺ regulatory T cells (2, 3). Its up-regulation in the hippocampus is associated with schizophrenia (4).

References