Recombinant Mouse BTNL10/Butyrophilin-like 10 Fc Protein, CF

Catalog #: 1444-BT Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
1444-BT-050

Recombinant Mouse BTNL10/Butyrophilin-like 10 Fc Protein Bioactivity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse BTNL10/Butyrophilin-like 10 Fc Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED₅₀ for this effect is 0.5-2.5 μg/mL.

Source

Mouse myeloma cell line, NS0-derived mouse BTNL10 protein

Mouse BTNL10 (Lys30-Glu167) Accession # Q9JK39	IEGRMDP	Mouse IgG_2a (Glu98-Lys330)
N-terminus		C-terminus

Accession #

Q9JK39

N-terminal Sequence
Analysis

Lys30

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

42.6 kDa

SDS-PAGE

58-63 kDa, reducing conditions

Product Datasheets

1444-BT

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1444-BT

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	12 months from date of receipt, ≤ -20 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Mouse BTNL10 Fc Chimera (Catalog # 1444-BT) inhibits IL-2 secretion by mouse T cells in the presence of anti-CD3 antibody. The ED₅₀ for this effect is 0.5-2.5 μg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Mouse BTNL10 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 58 - 63 kDa and 120 - 130 kDa, respectively.

Reconstitution Calculator

Background: BTNL10

Butyrophilin-like 10 (BTNL10) is a member of the BTN/MOG Ig-superfamily and functions as a negative regulator of immune cell activation (1). Mouse BTNL10 is a 275 amino acid (aa) type I transmembrane glycoprotein that contains a signal peptide followed by an extracellular domain (ECD), a transmembrane region, and a short cytoplasmic domain. The ECD of mouse BTNL10 features a single IgV and a poorly defined IgC domain. The IgV domain of mouse BTNL10 shares 53% and 52% sequence identity with the equivalent domain in human and rat BTNL10, respectively. Although recently identified, BTNL10 is one of only five butyrophilins conserved between human and mouse (2). While the complete immunological function of BTN/BTNL molecules is only beginning to emerge, they have been shown to be important in immunity by regulating T cell function (2-4). Recent efforts have focused on BTN/BTNL as potential therapeutic targets for a wide range of diseases (2-4). Currently, both the expression pattern and native function of BTNL10 remain unknown. Our data indicate that BTNL10 inhibits the human T cell activation, including IL-2, IFN-gamma secretion, and T cell proliferation.

References