Recombinant Mouse BTNL3 Fc Chimera Protein, CF

Catalog #: 10222-BT Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10222-BT-050

2 Images

Product Details

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse BTNL3 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED₅₀ for this effect is 0.4-4 μg/mL.

Source

Mouse myeloma cell line, NS0-derived mouse BTNL3 protein

Recombinant Mouse BTNL3 (Glu28-Pro241) Accession # Q7TST0.3	IEGRMDP	Mouse IgG_2a (Glu98-Lys330)
N-terminus		C-terminus

Accession #

Q7TST0.3

N-terminal Sequence
Analysis

Glu28

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

51 kDa

SDS-PAGE

45-65 kDa, under reducing conditions

Product Datasheets

10222-BT

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10222-BT

Formulation	Supplied as a 0.2 μm filtered solution in PBS.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after opening. 3 months, -20 to -70 °C under sterile conditions after opening.

Scientific Data

Bioactivity

View Larger

Measured by its ability to inhibit IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED₅₀ for this effect is 0.4-4 μg/mL.

SDS-PAGE

View Larger

2 μg/lane of Recombinant Mouse BTNL3 Fc Chimera (Catalog # 10222-BT) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 45-65 kDa and 90-130 kDa, respectively.

Reconstitution Calculator

Background: BTNL3

Butyrophilin-like 3 (BTNL3), also referred to as BTNL1, Gm316, and Ng10, is a member of the BTN/MOG Ig-superfamily and shares structural resemblance to the B7 family (1). BTNL3 is a type I transmembrane protein that consists of an extracellular domain (ECD) containing two IgV domains, a single transmembrane domain, and a cytoplasmic domain with a B30.2/SPRY region. Mature mouse BTNL3 shares 30% and 80% amino acid sequence identity with human and rat BTNL3, respectively. BTNL3 mRNA has been identified in many tissues including small intestine, colon, liver, lung, bone marrow and spleen tissue, and it is highly expressed in neutrophils (2-6). The specific function of BTNL3 has yet to be fully elucidated, but BTNL3 is suggested to be a novel negative regulator for T cell activation and immune diseases (7). In humans, BTNL3 has been shown to form heterodimers with BTNL8 and overexpression of this complex leads to CD69 upregulation and T cell antigen receptor downregulation (8). In mice, BTNL3 can form heterodimers with BTNL6 and modulate local immune responses and intraepithelial lymphocytes–epithelial cell interaction pathways (9). Additionally, BTNL3 has been shown to bind directly to V gamma 4+ T cell receptor, suggesting a role in gamma δ T cell regulation (10). BTNL3 expression has also been found to be down-regulated in colon cancer tumors (11). R&D Systems in house data indicate that mouse BTNL3 protein inhibits the secretion of IL-2 from anti-CD3 activated mouse CD3+ T cells.

References