Recombinant Mouse BTNL4 His-tag Protein, CF

Catalog #: 9594-BT Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9594-BT-050

Recombinant Mouse BTNL4 His-tag Protein Bioactivity

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Recombinant Mouse BTNL4 His-tag Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED₅₀ for this effect is 0.8-4 μg/mL.

Source

Mouse myeloma cell line, NS0-derived mouse BTNL4 protein
Gln29-Trp250, with a C-terminal 6-His tag

Accession #

NP_109671

N-terminal Sequence
Analysis

No results obtained. Gln29 inferred from enzymatic pyroglutamate treatment revealing Glu30

Predicted Molecular Mass

26 kDa

SDS-PAGE

34-39 kDa, reducing conditions

Product Datasheets

9594-BT

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9594-BT

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Mouse BTNL4 (Catalog # 9594-BT) inhibits IL-2 secretion by mouse T cells in the presence of anti-CD3 antibody. The ED₅₀ for this effect is 0.8-4 μg/mL.

Reconstitution Calculator

Background: BTNL4

Butyrophilin-like 4, also known as BTLN4, BTN3A3 (human) and BTN3A2 (rat) is an approximately 65 kDa member of the butyrophilin family that may play a role in immune regulation (1-3). The mouse BTNL4 is a 586 aa type 1 transmembrane protein, consisting of a 28 aa signal peptide, followed by a 109 aa immunoglobulin (Ig) V domain, a 71 aa IgC domain, a 20 aa transmembrane domain, and a 170 aa cytoplasmic B30.2/SPRY domain (4). Within the extracellular domain including aa 29‑250, mouse BTNL4 shares 45% and 82% sequence identity with human BTN3A3 and rat BTN3A2, respectively. BTNL4 shows striking sequence similarity to Skint1; is also largely restricted to an epithelial tissue (the small intestine) replete with T cells (4). Our in-house studies showed BTNL4 co-inhibited anti-CD3 induced IL‑2 secretion on CD3⁺ cells.

References