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Recombinant Mouse CCL19/MIP-3 beta Protein

Catalog #: 440-M3
39 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
440-M3-025/CF

With Carrier

Catalog # Availability Size / Price Qty
440-M3-025
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Citations (39)
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Recombinant Mouse CCL19/MIP-3 beta Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to chemoattract 5-10 day cultured human peripheral blood lymphocytes (PBL). The ED50 for this effect is 0.03‑0.1 µg/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR7. The ED50 for this effect is 3-15 ng/mL.
Source
E. coli-derived mouse CCL19/MIP-3 beta protein
Gly26-Val107-Leu-Glu, with a substitution of Ser108LeuGlu
Accession #
Q548P0
N-terminal Sequence
Analysis
Gly26
Predicted Molecular Mass
9.4 kDa

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440-M3 (with carrier)

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440-M3/CF (carrier free)

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COA
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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

440-M3

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

440-M3/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL19/MIP-3 beta

CCL19/MIP-3 beta, also known as ELC (EBI1-Ligand Chemokine), is a reported beta  chemokine that binds specifically to the chemokine receptor CCR‑7/EBI‑1/BLR‑2. Mouse (human) MIP-3 beta cDNA encodes a 108 (98) amino acid residue precursor protein with a predicted 25 (21) aa residue signal peptide that is cleaved to form the 83 (77) aa residue mature secreted protein. MIP-3 beta is distantly related to other beta chemokines (20 - 30% aa sequence identity). Mouse MIP-3 beta shares 83% aa sequence homology with human MIP-3 beta. MIP-3 beta  has been shown to be constitutively expressed in various lymphoid tissues (including thymus, lymph nodes, appendix, and spleen) in dendritic cells within the T-cell zone. The expression of MIP-3 beta is down‑regulated by the anti‑inflammatory cytokine IL‑10. Recombinant MIP-3 beta has been shown to be chemotactic for T cells and B cells. The MIP-3 beta receptor (CCR-7/EBI-1/BLR-2) is expressed in various lymphoid tissues and activated B and T lymphocytes. CCR-7 is also strongly up-regulated in B cells infected with Epstein-Barr virus and T cells infected with herpesvirus 6 or 7.

References
  1. Kim, C.H. et al. (1998) J. Immunol. 160:2418.
  2. Ngo, V.N. et al. (1998) J. Exp. Med. 188:181.
  3. Rossi, D.L. et al. (1997) J. Immunol. 158:1033.
  4. Yoshida, R. et al. (1997) J. Biol. Chem. 272:13803.
Entrez Gene IDs
6363 (Human); 24047 (Mouse); 362506 (Rat)
Alternate Names
beta chemokine exodus-3; Beta-chemokine exodus-3; CC chemokine ligand 19; C-C motif chemokine 19; CCL19; chemokine (C-C motif) ligand 19; CKb11; EBI1-ligand chemokine; ELC; ELCMIP-3-beta; Epstein-Barr virus-induced molecule 1 ligand chemokine; exodus-3; Macrophage inflammatory protein 3 beta; macrophage inflammatory protein 3-beta; MGC34433; MIP3 beta; MIP-3 beta; MIP-3b; MIP3BCK beta-11; SCYA19EBI1 ligand chemokine; small inducible cytokine subfamily A (Cys-Cys), member 19; Small-inducible cytokine A19

Citations for Recombinant Mouse CCL19/MIP-3 beta Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

39 Citations: Showing 1 - 10
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  1. Systematic identification of gene combinations to target in innate immune cells to enhance T cell activation
    Authors: Xia, L;Komissarova, A;Jacover, A;Shovman, Y;Arcila-Barrera, S;Tornovsky-Babeay, S;Jaya Prakashan, MM;Nasereddin, A;Plaschkes, I;Nevo, Y;Shiff, I;Yosefov-Levi, O;Izhiman, T;Medvedev, E;Eilon, E;Wilensky, A;Yona, S;Parnas, O;
    Nature communications
    Species: Mouse, Transgenic Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Influence of PEG Chain Length of Functionalized Magnetic Nanoparticles on the Cytocompatibility and Immune Competence of Primary Murine Macrophages and Dendritic Cells
    Authors: R Storjohann, B Gericke, J Reifenrath, T Herrmann, P Behrens, H Oltmanns, J Mei beta ner
    International Journal of Molecular Sciences, 2023-01-29;24(3):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Studies with neutralizing antibodies suggest CXCL8-mediated neutrophil activation is independent of C-C motif chemokine receptor-like 2 (CCRL2) ligand binding function
    Authors: Z Su, J Brooks, J Pelker, T Andreyeva, H Sobon, R Gifford, M Powers, J Wang, C Dower, M Hegen, D Messing, AD Sheehan, JJ Brennan
    PLoS ONE, 2023-01-20;18(1):e0280590.
    Species: Human
    Sample Types: Transfected Whole Cells
    Applications: Bioassay
  4. Glycosyltransferase Extl1 promotes CCR7-mediated dendritic cell migration to restrain infection and autoimmunity
    Authors: J Liu, Y Cheng, X Zhang, Y Chen, H Zhu, K Chen, S Liu, Z Li, X Cao
    Cell Reports, 2023-01-18;42(1):111991.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Influence of PEG Chain Length of Functionalized Magnetic Nanoparticles on the Cytocompatibility and Immune Competence of Primary Murine Macrophages and Dendritic Cells
    Authors: R Storjohann, B Gericke, J Reifenrath, T Herrmann, P Behrens, H Oltmanns, J Mei beta ner
    International Journal of Molecular Sciences, 2023;24(3):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. GPR174 signals via Galphas to control a CD86-containing gene expression program in B cells
    Authors: EW Wolf, ZP Howard, L Duan, H Tam, Y Xu, JG Cyster
    Proceedings of the National Academy of Sciences of the United States of America, 2022-05-31;119(23):e2201794119.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. PD-L1 signaling selectively regulates T cell lymphatic transendothelial migration
    Authors: W Piao, L Li, V Saxena, J Iyyathurai, R Lakhan, Y Zhang, IT Lape, C Paluskievi, KL Hippen, Y Lee, E Silverman, MW Shirkey, LV Riella, BR Blazar, JS Bromberg
    Nature Communications, 2022-04-21;13(1):2176.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Treg tissue stability depends on lymphotoxin beta-receptor- and adenosine-receptor-driven lymphatic endothelial cell responses
    Authors: V Saxena, W Piao, L Li, C Paluskievi, Y Xiong, T Simon, R Lakhan, CC Brinkman, S Walden, KL Hippen, M WillsonShi, YS Lee, C Wagner, BR Blazar, JS Bromberg
    Cell Reports, 2022-04-19;39(3):110727.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  9. The lysosomal Ragulator complex plays an essential role in leukocyte trafficking by activating myosin II
    Authors: T Nakatani, K Tsujimoto, J Park, T Jo, T Kimura, Y Hayama, H Konaka, T Morita, Y Kato, M Nishide, S Koyama, S Nada, M Okada, H Takamatsu, A Kumanogoh
    Nature Communications, 2021-06-07;12(1):3333.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Naringenin Attenuates Experimental Autoimmune Encephalomyelitis by Protecting the Intact of Blood-Brain Barrier and Controlling Inflammatory Cell Migration
    Authors: X Niu, H Sang, J Wang
    J Nutr Biochem, 2020-11-26;0(0):108560.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Nuclear positioning facilitates amoeboid migration along the path of least resistance
    Authors: J Renkawitz, A Kopf, J Stopp, I de Vries, MK Driscoll, J Merrin, R Hauschild, ES Welf, G Danuser, R Fiolka, M Sixt
    Nature, 2019-04-03;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Role of microRNA in CB1 antagonist-mediated regulation of adipose tissue macrophage polarization and chemotaxis during diet-induced obesity
    Authors: P Mehrpouya-, K Miranda, NP Singh, EE Zumbrun, M Nagarkatti, PS Nagarkatti
    J. Biol. Chem., 2019-03-25;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Drp1 Controls Effective T Cell Immune-Surveillance by Regulating T Cell Migration, Proliferation, and cMyc-Dependent Metabolic Reprogramming
    Authors: L Simula, I Pacella, A Colamatteo, C Procaccini, V Cancila, M Bordi, C Tregnago, M Corrado, M Pigazzi, V Barnaba, C Tripodo, G Matarese, S Piconese, S Campello
    Cell Rep, 2018-12-11;25(11):3059-3073.e10.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Regulation of T cell afferent lymphatic migration by targeting LT?R-mediated non-classical NF?B signaling
    Authors: W Piao, Y Xiong, K Famulski, CC Brinkman, L Li, N Toney, C Wagner, V Saxena, T Simon, JS Bromberg
    Nat Commun, 2018-08-01;9(1):3020.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Atypical chemokine receptor 4 shapes activated B cell fate
    Authors: EE Kara, CR Bastow, DR McKenzie, CE Gregor, KA Fenix, R Babb, TS Norton, D Zotos, LB Rodda, JR Hermes, K Bourne, DS Gilchrist, RJ Nibbs, M Alsharifi, CG Vinuesa, DM Tarlinton, R Brink, GR Hill, JG Cyster, I Comerford, SR McColl
    J. Exp. Med., 2018-01-31;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  16. The Small Rho GTPase TC10 Modulates B Cell Immune Responses
    Authors: M Burbage, SJ Keppler, B Montaner, PK Mattila, FD Batista
    J. Immunol., 2017-07-26;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Concurrent CCR7 Overexpression and RelB Knockdown in Immature Dendritic Cells Induces Immune Tolerance and Improves Skin-Graft Survival in a Murine Model
    Authors: Z Dong, Y Chen, Y Peng, F Wang, Z Yang, G Huang, Y Chen, Z Yuan, T Cao, Y Peng
    Cell. Physiol. Biochem., 2017-06-05;42(2):455-468.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Adenovirus-Mediated CCR7 and BTLA Overexpression Enhances Immune Tolerance and Migration in Immature Dendritic Cells
    Authors: H Xin, J Zhu, H Miao, Z Gong, X Jiang, X Feng, Y Tong
    Biomed Res Int, 2017-03-14;2017(0):3519745.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  19. Normal Thymocyte Egress, T Cell Trafficking, and CD4(+) T Cell Homeostasis Require Interactions between RGS Proteins and G?i2
    Authors: IY Hwang, C Park, K Harrison, JH Kehrl
    J. Immunol, 2017-02-24;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  20. A reverse signaling pathway downstream of Sema4A controls cell migration via Scrib
    Authors: Tianliang Sun
    J. Cell Biol, 2016-12-22;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  21. The Chemokine Receptor CX3CR1 Defines Three Antigen-Experienced CD8�T Cell Subsets with Distinct Roles in Immune Surveillance and Homeostasis
    Authors: Carmen Gerlach
    Immunity, 2016-12-06;45(6):1270-1284.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  22. Immunogenic Dendritic Cell Generation from Pluripotent Stem Cells by Ectopic Expression of Runx3
    Authors: Istvan Szatmari
    J. Immunol., 2016-11-16;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  23. A microfluidic device for measuring cell migration towards substrate-bound and soluble chemokine gradients
    Sci Rep, 2016-11-07;6(0):36440.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  24. CCL19-CCR7-dependent reverse transendothelial migration of myeloid cells clears Chlamydia muridarum from the arterial intima
    Nat Immunol, 2016-09-26;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  25. Characterization of the Expression and Function of the C-Type Lectin Receptor CD302 in Mice and Humans Reveals a Role in Dendritic Cell Migration
    Authors: Tsun-Ho Lo
    J Immunol, 2016-06-17;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  26. Hyaluronan Binding Identifies a Functionally Distinct Alveolar Macrophage-like Population in Bone Marrow-Derived Dendritic Cell Cultures.
    Authors: Poon G, Dong Y, Marshall K, Arif A, Deeg C, Dosanjh M, Johnson P
    J Immunol, 2015-06-17;195(2):632-42.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  27. An essential role for RGS protein/Galphai2 interactions in B lymphocyte-directed cell migration and trafficking.
    Authors: Hwang I, Park C, Harrison K, Boularan C, Gales C, Kehrl J
    J Immunol, 2015-01-23;194(5):2128-39.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  28. A potential protein adjuvant derived from Mycobacterium tuberculosis Rv0652 enhances dendritic cells-based tumor immunotherapy.
    Authors: Lee S, Shin S, Lee M, Lee M, Kang T, Park W, Soh B, Park J, Shin Y, Kim H, Yun C, Jung I, Park Y
    PLoS ONE, 2014-08-07;9(8):e104351.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  29. Enhancement of tumor-specific T cell-mediated immunity in dendritic cell-based vaccines by Mycobacterium tuberculosis heat shock protein X.
    Authors: Jung I, Shin S, Lee M, Kang T, Han H, Lee S, Kim W, Kim H, Park W, Kim H, Yun C, Lee E, Wu T, Park Y
    J Immunol, 2014-07-02;193(3):1233-45.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  30. Netrin-1 promotes adipose tissue macrophage retention and insulin resistance in obesity.
    Authors: Ramkhelawon B, Hennessy E, Menager M, Ray T, Sheedy F, Hutchison S, Wanschel A, Oldebeken S, Geoffrion M, Spiro W, Miller G, McPherson R, Rayner K, Moore K
    Nat Med, 2014-03-02;20(4):377-84.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  31. Rgs13 constrains early B cell responses and limits germinal center sizes.
    Authors: Hwang I, Hwang K, Park C, Harrison K, Kehrl J
    PLoS ONE, 2013-03-22;8(3):e60139.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  32. Neuroimmune guidance cue Semaphorin 3E is expressed in atherosclerotic plaques and regulates macrophage retention.
    Authors: Wanschel, Amarylis, Seibert, Tara, Hewing, Bernd, Ramkhelawon, Bhama, Ray, Tathagat, van Gils, Janine M, Rayner, Katey J, Feig, Jonathan, O'Brien, Edward R, Fisher, Edward A, Moore, Kathryn
    Arterioscler Thromb Vasc Biol, 2013-02-21;33(5):886-93.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  33. Influence of hypoxia-inducible factor 1-alpha on dendritic cell differentiation and migration.
    Authors: Kohler T, Reizis B, Johnson RS, Weighardt H, Forster I
    Eur. J. Immunol., 2012-05-01;42(5):1226-36.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  34. Cooperative function of CCR7 and lymphotoxin in the formation of a lymphoma-permissive niche within murine secondary lymphoid organs.
    Authors: Rehm A, Mensen A, Schradi K, Gerlach K, Wittstock S, Winter S, Buchner G, Dorken B, Lipp M, Hopken UE
    Blood, 2011-05-17;118(4):1020-33.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  35. CCR2 plays a critical role in dendritic cell maturation: possible role of CCL2 and NF-kappa B.
    Authors: Jimenez F, Quinones MP, Martinez HG, Estrada CA, Clark K, Garavito E, Ibarra J, Melby PC, Ahuja SS
    J. Immunol., 2010-04-19;184(10):5571-81.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  36. Phosphatidylinositol-3-OH kinase and nutrient-sensing mTOR pathways control T lymphocyte trafficking.
    Authors: Sinclair LV, Finlay D, Feijoo C, Cornish GH, Gray A, Ager A, Okkenhaug K, Hagenbeek TJ, Spits H, Cantrell DA
    Nat. Immunol., 2008-04-06;9(5):513-21.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  37. CCR7 ligands stimulate the intranodal motility of T lymphocytes in vivo.
    Authors: Worbs T, Mempel TR, Bolter J, von Andrian UH, Forster R
    J. Exp. Med., 2007-02-26;204(3):489-95.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  38. Role of CCL21 and CCL19 in allergic inflammation in the ovalbumin-specific murine asthmatic model.
    Authors: Yamashita N, Tashimo H, Matsuo Y, Ishida H, Yoshiura K, Sato K, Kakiuchi T, Ohta K
    J. Allergy Clin. Immunol., 2006-05-01;117(5):1040-6.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  39. The role of CCL21 in recruitment of T-precursor cells to fetal thymi.
    Authors: Ueno T, Kuse S, Saito F, Nitta T, Kakiuchi T, Hollander GA, Takahama Y
    Blood, 2004-09-09;105(1):31-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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