Recombinant Mouse CCL22/MDC Protein

Catalog #: 439-MD
9 Citations Datasheet / COA / SDS

Carrier Free

Catalog #	Availability	Size / Price	Qty
439-MD-025/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
439-MD-025

R&D Systems Recombinant Proteins and Enzymes

1 Image

Product Details

Citations (9)

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse CCL22/MDC Protein Summary

Product Specifications

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR4. The ED₅₀ for this effect is 0.5-3 ng/mL.

Source

E. coli-derived mouse CCL22/MDC protein
Gly25-Ser92

Accession #

Q9QZU2

N-terminal Sequence
Analysis

Gly25

Predicted Molecular Mass

7.8 kDa

Product Datasheets

439-MD (with carrier)

Product Datasheet

COA

SDS

439-MD/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

439-MD

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution	Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

439-MD/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: CCL22/MDC

Mouse ABCD-1, the mouse orthologue of the human macrophage-derived chemokine (MDC)/stimulated T cell chemotactic protein (STCP-1), is a CC chemokine cloned from activated mouse B cells. Mouse MDC cDNA encodes a precursor protein of 92 amino acid (aa) residues with a 24 aa residue predicted signal peptide that is cleaved to yield a 68 aa residue mature 7.8 kDa protein. At the amino acid sequence level, mouse and human MDC share 64% identity and 83% similarity. The genomic organization of the mouse and human MDC genes are very similar, exhibiting sequence identity at the intron-exon boundaries. Mouse MDC is expressed at high levels in dendritic cells and activated B lymphocytes. Low levels of mouse MDC mRNA are also detectable in lung, unstimulated spleen cells, lymph node cells and in thymocytes. MDC is a functional ligand for the CC chemokine receptor 4. Recombinant or chemically synthesized mature mouse MDC has been shown to induce chemotaxis or Ca²⁺ mobilization in activated mouse and human T cells.

References

Schaniel, C. et al. (1998) J. Exp. Med. 188:451.
Imai, T. et al. (1998) J. Biol. Chem. 273:1764.
Godiska, R. et al. (1997) J. Exp. Med. 185:1595.
Chang, M-S. et al. (1997) J. Biol. Chem. 272:25229.

Entrez Gene IDs

6367 (Human); 20299 (Mouse)

Alternate Names

A-152E5.1; ABCD-1; CC chemokine STCP-1; C-C motif chemokine 22; CCL22; chemokine (C-C motif) ligand 22; DC/B-CK; Macrophage-derived chemokine; MDC; MDCStimulated T-cell chemotactic protein 1; MGC34554; SCYA22MDC(1-69); small inducible cytokine A22; small inducible cytokine subfamily A (Cys-Cys), member 22; Small-inducible cytokine A22; STCP-1; stimulated T cell chemotactic protein 1

Related Research Areas

Citations for Recombinant Mouse CCL22/MDC Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
Filter your results:

Filter by:

Pexidartinib synergize PD-1 antibody through inhibiting treg infiltration by reducing TAM-derived CCL22 in lung adenocarcinoma
Authors: W Zhang, X Jiang, Y Zou, L Yuan, X Wang
Frontiers in Pharmacology, 2023-03-08;14(0):1092767.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo
Regulatory T Cells Exhibit Interleukin-33-Dependent Migratory Behavior during Skin Barrier Disruption
Authors: S Toyama, CS Moniaga, S Nakae, M Kurosawa, H Ogawa, M Tominaga, K Takamori
International Journal of Molecular Sciences, 2021-07-12;22(14):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
Chemokine-Binding Proteins Encoded by Parapoxvirus of Red Deer of New Zealand Display Evidence of Gene Duplication and Divergence of Ligand Specificity
Authors: S Sharif, N Ueda, Y Nakatani, LM Wise, S Clifton, Z Lateef, AA Mercer, SB Fleming
Front Microbiol, 2019-06-25;10(0):1421.
Species: Mouse
Sample Types:
Applications: Binding Assay
Treg-recruiting microspheres prevent inflammation in a murine model of dry eye disease
Authors: ML Ratay, AJ Glowacki, SC Balmert, AP Acharya, J Polat, LP Andrews, MV Fedorchak, JS Schuman, DAA Vignali, SR Little
J Control Release, 2017-05-10;0(0):.
Species: Mouse
Sample Types: Recombinant Protein
Applications: Bioassay, In Vivo
A novel murine cytomegalovirus vaccine vector protects against Mycobacterium tuberculosis.
Authors: Beverley P, Ruzsics Z, Hey A, Hutchings C, Boos S, Bolinger B, Marchi E, O'Hara G, Klenerman P, Koszinowski U, Tchilian E
J Immunol, 2014-07-28;193(5):2306-16.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo
Defective immunoregulation in RSV vaccine-augmented viral lung disease restored by selective chemoattraction of regulatory T cells.
Authors: Loebbermann, Jens, Durant, Lydia, Thornton, Hannah, Johansson, Cecilia, Openshaw, Peter J
Proc Natl Acad Sci U S A, 2013-02-04;110(8):2987-92.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo
Resolution of allergic inflammation and airway hyperreactivity is dependent upon disruption of the T1/ST2-IL-33 pathway.
Authors: Kearley J, Buckland KF, Mathie SA, Lloyd CM
Am. J. Respir. Crit. Care Med., 2009-01-29;179(9):772-81.
Applications: ELISA (Standard)
CD4+CD25+ regulatory T cells reverse established allergic airway inflammation and prevent airway remodeling.
Authors: Kearley J, Robinson DS, Lloyd CM
J. Allergy Clin. Immunol., 2008-07-30;122(3):617-24.e6.
Applications: ELISA (Standard)
The direct action of 1alpha,25(OH)2-vitamin D3 on purified mouse Langerhans cells.
Authors: Fujita H, Asahina A, Komine M, Tamaki K
Cell. Immunol., 2007-05-15;245(2):70-9.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: Bioassay