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Recombinant Mouse CCL7/MARC Protein

Catalog #: 456-MC
9 Citations 1 Review Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
456-MC-010/CF
456-MC-050/CF

With Carrier

Catalog # Availability Size / Price Qty
456-MC-010
456-MC-050
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (9)
FAQs
Reviews (1)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse CCL7/MARC Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to chemoattract 2-day cultured human monocytes. Matsushima, K. et al. (1989) J. Exp. Med. 169:1485. The ED50 for this effect is 0.1-0.3 µg/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR2A. The ED50 for this effect is 0.5‑2 µg/mL.
Source
E. coli-derived mouse CCL7/MCP-3/MARC protein
Gln24-Pro97
Accession #
Q03366.1
N-terminal Sequence
Analysis
Gln24
Predicted Molecular Mass
8.5 kDa

Product Datasheets

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456-MC (with carrier)

Product Datasheet
COA
SDS

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456-MC/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

456-MC

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile, deionized water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

456-MC/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile, deionized water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL7/MCP-3/MARC

Mouse MARC, a member of the beta  subfamily of chemokines, was initially identified as a transcript that is induced in a mouse mast cell line after Fc epsilon RI triggering by IgE plus antigen. Sequence comparisons suggest that MARC may be the mouse homologue of the human MCP-3 gene. Mouse MARC/MCP-3 expression has also been detected during murine experimental allergic encephalomyelitis in the spinal cord, and in LPS-stimulated murine WEHI -3 cells and Swiss 3T3 cells where MARC expression is glucocorticoid-attenuated. Except for one amino acid subsititution, mouse MARC is identical to mouse FIC, the product of a growth factor-activated gene. The mouse MARC cDNA encodes a 97 amino acid residue precursor protein with a 23 amino acid residue signal peptide that is cleaved to yield a 74 amino acid residue mature protein. Mouse CCR2, a mouse chemokine receptor, has been shown to bind JE/MCP-1 with high affinity and MARC/MCP-3 with lower affinity. The E. coli-expressed mouse MARC/MCP-3 produced at R&D Systems has been shown to be a monocyte and T-lymphocyte chemoattractant.

References
  1. Kulmburg, P.A. et al. (1992) J. Exp. Med. 176:1773.
  2. Thirion, S. et al. (1994) Biochem. Biophys. Res. Commun. 201:493.
  3. Smith, J.B. and H.R. Herschman (1995) J. Biol. Chem. 270:16756.
  4. Kurihara, T. and R. Bravo (1996) J. Biol. Chem. 271:11603.
Entrez Gene IDs
6354 (Human); 20306 (Mouse)
Alternate Names
C-C motif chemokine 7; CCL7; chemokine (C-C motif) ligand 7; FIC; MARC; MCP-3; MCP3Monocyte chemotactic protein 3; MCP-3Small-inducible cytokine A7; MGC138465; Monocyte chemoattractant protein 3; NC28SCYA6; SCYA7MGC138463; small inducible cytokine A7 (monocyte chemotactic protein 3)

Citations for Recombinant Mouse CCL7/MARC Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Sepsis-trained macrophages promote antitumoral tissue-resident T cells
    Authors: Broquet, A;Gourain, V;Goronflot, T;Le Mabecque, V;Sinha, D;Ashayeripanah, M;Jacqueline, C;Martin, P;Davieau, M;Boutin, L;Poulain, C;Martin, FP;Fourgeux, C;Petrier, M;Cannevet, M;Leclercq, T;Guillonneau, M;Chaumette, T;Laurent, T;Harly, C;Scotet, E;Legentil, L;Ferrières, V;Corgnac, S;Mami-Chouaib, F;Mosnier, JF;Mauduit, N;McWilliam, HEG;Villadangos, JA;Gourraud, PA;Asehnoune, K;Poschmann, J;Roquilly, A;
    Nature immunology
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Studies with neutralizing antibodies suggest CXCL8-mediated neutrophil activation is independent of C-C motif chemokine receptor-like 2 (CCRL2) ligand binding function
    Authors: Z Su, J Brooks, J Pelker, T Andreyeva, H Sobon, R Gifford, M Powers, J Wang, C Dower, M Hegen, D Messing, AD Sheehan, JJ Brennan
    PLoS ONE, 2023-01-20;18(1):e0280590.
    Species: Human
    Sample Types: Transfected Whole Cells
    Applications: Bioassay
  3. -->The CCL2/CCL7/CCL12/CCR2 pathway is substantially and persistently upregulated in mice after traumatic brain injury, and CCL2 modulates the complement system in microglia
    Authors: K Popiolek-B, A Ciechanows, K Ciapa?a, K Pawlik, M Oggioni, D Mercurio, MG De Simoni, J Mika
    Mol Cell Probes, 2020-11-04;54(0):101671.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  4. Regulatory T Cells Play a Role in a Subset of Idiopathic Preterm Labor/Birth and Adverse Neonatal Outcomes
    Authors: N Gomez-Lope, M Arenas-Her, R Romero, D Miller, V Garcia-Flo, Y Leng, Y Xu, J Galaz, SS Hassan, CD Hsu, H Tse, C Sanchez-To, B Done, AL Tarca
    Cell Rep, 2020-07-07;32(1):107874.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  5. Chemokines CCL2 and CCL7, but not CCL12, play a significant role in the development of pain-related behavior and opioid-induced analgesia
    Authors: K Kwiatkowsk, K Popiolek-B, A Piotrowska, E Rojewska, K Ciapa?a, W Makuch, J Mika
    Cytokine, 2019-04-16;119(0):202-213.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  6. Role of CCL7 in Type I Hypersensitivity Reactions in Murine Experimental Allergic Conjunctivitis
    Authors: Chuan-Hui Kuo
    J. Immunol, 2016-12-12;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Defective TGF-beta signaling in bone marrow-derived cells prevents hedgehog-induced skin tumors.
    Authors: Fan Q, Gu D, Liu H, Yang L, Zhang X, Yoder M, Kaplan M, Xie J
    Cancer Res, 2013-11-26;74(2):471-83.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  8. The role of CCL21 in recruitment of T-precursor cells to fetal thymi.
    Authors: Ueno T, Kuse S, Saito F, Nitta T, Kakiuchi T, Hollander GA, Takahama Y
    Blood, 2004-09-09;105(1):31-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Transcription regulation of TNF-alpha-early response genes by poly(ADP-ribose) polymerase-1 in murine heart endothelial cells.
    Authors: Carrillo A, Monreal Y, Ramirez P, Marin L, Parrilla P, Oliver FJ, Yelamos J
    Nucleic Acids Res., 2004-02-03;32(2):757-66.
    Applications: ELISA (Standard)

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Recombinant Mouse CCL7/MARC Protein
By jayeeta giri on 01/28/2019
Application: Enzymatic activity in vitro