Recombinant Mouse CD300g/Nepmucin Fc Chimera Protein, CF

Catalog #: 10801-NE Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10801-NE-050

Recombinant Mouse CD300g/Nepmucin Fc Chimera Protein Bioactivity.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse CD300g/Nepmucin Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells. The ED₅₀ for this effect is 0.40-4.00 μg/mL.

Source

Mouse myeloma cell line, NS0-derived mouse CD300g/Nepmucin protein

Mouse CD300g/Nepmucin (Leu19-Ile284) Accession # NP_001154183.1	IEGRMDP	Mouse IgG_2a (Glu98-Lys330)
N-terminus		C-terminus

Accession #

NP_001154183.1

N-terminal Sequence
Analysis

Leu19

Predicted Molecular Mass

55 kDa

SDS-PAGE

88-100 kDa, under reducing conditions

Product Datasheets

10801-NE

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10801-NE

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion by human T cells. The ED₅₀ for this effect is 0.40-4.0 μg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Mouse CD300g/Nepmucin Fc Chimera (Catalog # 10801-NE) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 88-100 kDa and 176-200 kDa, respectively.

Reconstitution Calculator

Background: CD300g/Nepmucin

Nepmucin, also known as CMRF35-like molecule 9 (CLM-9), TREM-4 and CD300g, is a 75-95 kDa type I transmembrane O-glycosylated member of the CD300 family of molecules. Nepmucin (mucin not expressed in Peyer's Patches) is expressed on capillary endothelium and its expression appears to be influenced by factors that are locally produced in tissues (1, 2). Nepmucin contains a single V-type immunoglobulin domain and a mucin-like domain (3). Mature human Nepmucin is composed of 313 amino acid (aa), containing 186 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 106 aa cytoplasmic region. Within the ECD, mouse nepmucin shares 53% aa identity with human Nepmucin. Nepmucin plays a critical role in promoting lymphocyte transendothelial migration of high endothelial veins and mediates lymphocyte adhesion with endothelial cells by its Ig domain, which is independent of LFA-1 or VLA-4 adhesion pathway (3). Glycosylated Nepmucin with MECA-79 epitope associate with L-selectin to mediate L-selectin-dependent lymphocyte rolling by its mucin-like domain (4). Expression of Nepmucin is down-regulated in tumors and tumor-draining lymph nodes, indicating that the expression of Nepmucin is negatively regulated by locally produced signals under these circumstances (2). Nepmucin induction can significantly improve the killing activity of CIK cells (5).

References

Jin, S. et al. (2008) FEBS Lett. 582:3018.
Umemoto, E. et al. (2013) PloS One. 8:e83681.
Umemoto, E. et al. (2006) J. Exp. Med. 203:1603.
Takatsu, H, et al. (2006) Biochem. Biophys. Res. Commun. 348:183.
Wang, Q. et al. (2017) Cent. Eur. J. Immunol. 42:117.

Entrez Gene IDs

146894 (Human); 52685 (Mouse); 684984 (Rat)

Alternate Names

CD300 antigen like family member G; CD300 antigen-like family member G; CD300 molecule like family member g; CD300 molecule-like family member g; CD300g antigen; CD300g; CD300LG; CLM9; CLM-9; CLM9Trem4; CMRF35-like molecule 9; Nepmucin; TREM4; TREM-4; Triggering receptor expressed on myeloid cells 4