Recombinant Mouse CD320/TCblR/8D6A Fc Chimera Protein, CF

Catalog #: 7190-CD Datasheet / COA / SDS

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7190-CD-050

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse CD320/TCblR/8D6A Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to bind plasma Cobalamin (Cbl) bound Transcobalamin (TC). Quadros, E.V. et al. (2009) Blood 113:186. When Recombinant Mouse CD320/TCblR/8D6A Fc Chimera is coated at 1 μg/mL (100 μL/well), it can bind human plasma TC (dilutions from 1:6 to 1:4000) in the presence of Cbl.

Source

Mouse myeloma cell line, NS0-derived mouse CD320/TCblR/8D6A protein

Mouse CD320 (Met1 - Gly208) Accession # Q9Z1P5	IEGRMDP	Mouse IgG_2A (Glu98 - Lys330)
N-terminus		C-terminus

Accession #

Q9Z1P5

N-terminal Sequence
Analysis

Ala29

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

46.7 kDa (monomer)

SDS-PAGE

70-90 kDa, reducing conditions

Product Datasheets

7190-CD

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7190-CD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: CD320/TCblR/8D6A

CD320, also known as the Transcobalamin II receptor (TCblR) and 8D6A, is an approximately 60 kDa transmembrane member of the LDL receptor family (1). Mature mouse CD320 consists of a 180 amino acid (aa) extracellular domain with two cysteine-rich LDLR-A repeats, a 21 aa transmembrane segment, and a 31 aa cytoplasmic domain (2, 3). Within the extracellular domain, mouse CD320 shares 52% aa sequence identity with human and rat CD320. Alternate splicing of mouse CD320 generates an additional isoform with a substitution of the N-terminal 40 aa. CD320 is a heavily glycosylated protein that is expressed as a noncovalent dimer in the kidney, placenta, liver, intestine, and secondary lymphoid organs (3 - 5). It functions as an endocytic receptor for circulating complexes of Cobalamin (Vitamin B₁₂) and Transcobalamin II (3). In the immune system, CD320 is expressed by germinal center B cells and follicular dendritic cells (FDC) and provides a costimulatory signal to B cells during the germinal center reaction (2, 6 - 8). CD320 also cooperates with CD44 in promoting the development of B cell lymphomas (9).

References