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Recombinant Mouse CD5L Protein, CF

Catalog #: 2834-CL
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
2834-CL-050
R&D Systems Recombinant Proteins and Enzymes
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Citations (3)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse CD5L Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit apoptosis on the J774A.1 mouse reticulum cell sarcoma macrophage cells. Miyazaki, T. et al. (1999) J. Exp. Med. 189:413. The presence of rhCD5L inhibited rhFas Ligand/His-induced apoptosis by 70-90% in the presence of 10 µg/mL of a cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050). The ED50 for this effect is
1-4 µg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse CD5L protein
Glu22-Val352, with a C-terminal 6-His tag
Accession #
Q9QWK4
N-terminal Sequence
Analysis
Glu22
Predicted Molecular Mass
37.4 kDa (monomer)
SDS-PAGE
55-61 kDa, reducing conditions

Product Datasheets

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2834-CL

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2834-CL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD5L

CD5L (CD5 antigen-like), also known as Sp alpha and AIM, is a 50 kDa secreted glycoprotein that belongs to the SRCR (scavenger receptor cysteine-rich) group B family of proteins. Group B proteins are distinguished by SRCR domains that are encoded by a single exon (1 - 3). The human CD5L cDNA encodes a 347 amino acid (aa) precursor that includes a 19 aa signal sequence and three SRCR domains (4, 5). Among group B proteins, CD5L is most closely related to CD5 and CD6, with which it shares 18% and 31% aa sequence identity, respectively. CD5L is upregulated in macrophages at inflammatory sites. It sustains inflammatory reactions by both increasing the phagocytic capacity of macrophages and impeding the apoptosis of local macrophages, NK cells, and T cells (6, 7). Agonists of the LXR and RXR nuclear hormone receptors induce CD5L upregulation in macrophages and reduce macrophage apoptosis (8, 9). Oxidized LDL (which acts through LXR/RXR) is taken up by macrophages, promoting their development into foam cells. The increased level of CD5L protects foam cells from apoptosis but permits more rapid cellular accumulation and atherosclerotic plaque formation (9). In activated B cells, however, the combination of CD5L and TGF-beta inhibits proliferation. The binding of CD5L to splenic B cells is increased following TGF-beta exposure, suggesting that TGF-beta increases the expression or availability of an unidentified CD5L receptor (5, 10). CD5L also functions as a pattern recognition molecule by binding both lipoteichoic acid on Gram positive and lipopolysaccharide on Gram negative bacteria (11). In the thymic cortex, CD5L protects cortical CD4+CD8+ thymocytes from apoptosis (12). CD5L circulates in the serum in complex with IgM (13).

References
  1. Sarrias, M.R. et al. (2004) Crit. Rev. Immunol. 24:1.
  2. Resnick, D. et al. (1994) Trends Biochem. Sci. 19:5.
  3. Mukhopadhyay, S. and S. Gordon (2004) Immunobiology 209:39.
  4. Gebe, J.A. et al. (1997) J. Biol. Chem. 272:6151.
  5. Gebe, J.A. et al. (2000) Immunology. 99:78.
  6. Haruta, I. et al. (2001) J. Biol. Chem. 276:22910.
  7. Kuwata, K. et al. (2003) Am. J. Pathol. 162:837.
  8. Valledor, A.F. et al. (2004) Proc. Natl. Acad. Sci. 101:17813.
  9. Arai, S. et al. (2005) Cell Metab. 1:201.
  10. Yusa, S. et al. (1999) Eur. J. Immunol. 29:1086.
  11. Sarrias, M-R. et al. (2005) J. Biol. Chem. 280:35391.
  12. Miyazaki, T. et al. (1999) J. Exp. Med. 189:413.
  13. Tissot, J.D. et al. (2002) Electrophoresis 23:1203.
Long Name
CD5 Antigen-like
Entrez Gene IDs
922 (Human); 11801 (Mouse)
Alternate Names
AIM; API6; apoptosis inhibitor 6; CD5 antigen-like (scavenger receptor cysteine rich family); CD5 antigen-like; CD5 molecule-like; CD5L; CT-2; IgM-associated peptide; PRO229; Spalpha; SP-alpha

Citations for Recombinant Mouse CD5L Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Macrophage CD5L is a target for cancer immunotherapy
    Authors: L Sanchez-Mo, T Paul, C Martori, J Font-Díaz, L Sanjurjo, G Aran, É Téllez, J Blanco, J Carrillo, M Ito, M Tuttolomon, HJ Ditzel, C Fumagalli, G Tapia, J Sidorova, H Masnou, MA Fernández-, JJ Lozano, C Vilaplana, A Rodriguez-, C Armengol, AF Valledor, L Kremer, MR Sarrias
    EBioMedicine, 2023-04-11;91(0):104555.
    Species: Mouse
    Sample Types:
    Applications: ELISA Capture
  2. Free IL-12p40 monomer is a polyfunctional adaptor for generating novel IL-12-like heterodimers extracellularly.
    Authors: Abdi K, Singh N, Spooner E, Kessler B, Radaev S, Lantz L, Xiao T, Matzinger P, Sun P, Ploegh H
    J Immunol, 2014-05-12;192(12):6028-36.
    Species: Mouse
    Sample Types:
    Applications: Bioassay
  3. Api6/AIM/Sp{alpha}/CD5L Overexpression in Alveolar Type II Epithelial Cells Induces Spontaneous Lung Adenocarcinoma.
    Authors: Li Y, Qu P, Wu L, Li B, Du H, Yan C
    Cancer Res., 2011-06-22;71(16):5488-99.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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