Recombinant Mouse Chondroadherin His-tag Protein, CF

Catalog #: 10033-CH Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10033-CH-050

Recombinant Mouse Chondroadherin His-tag Protein Bioactivity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Chondroadherin His-tag Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED₅₀ for this effect is 0.08-048 μg/mL

Source

Mouse myeloma cell line, NS0-derived mouse Chondroadherin protein
Ala21-Thr349, with a C-terminal 6-His tag

Accession #

O55226

N-terminal Sequence
Analysis

No results obtained. Protein identity confirmed via Mass Spectrometry.

Predicted Molecular Mass

38 kDa

SDS-PAGE

33-38 kDa, reducing conditions

Product Datasheets

10033-CH

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10033-CH

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, ≤ -20 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Mouse Chondroadherin (Catalog # 10033-CH) induces adhesion of ATDC5 mouse chondrogenic cells. The ED₅₀ for this effect is 0.08-0.48 μg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Mouse Chondroadherin was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 33-38 kDa.

Reconstitution Calculator

Background: Chondroadherin

Chondroadherin (CHAD), also known as SLRR4A, is a 38 kDa secreted protein belonging to the small leucine-rich proteoglycans (SLRPs) that help regulate the assembly and function of the extracellular matrix (ECM) (1). CHAD is highly expressed in cartilaginous tissues, with lower expression levels found in bone, tendon, and eye (2-4). Mature mouse CHAD is a 338 amino acid (aa) protein that contains twelve leucine-rich repeats (LRRs) including ten tandem leucine-rich repeats as well as N-terminal and C-terminal leucine-rich domains flanked by cysteine-rich regions. CHAD interacts with collagen II and mediates signaling between chondrocytes and the ECM by binding to the alpha 2 beta 1 integrin, heparan sulphate, and to cell surface proteoglycans like syndecans (5-9). In addition, CHAD also interacts with both N- and C-terminal globular domains of type VI collagen (10). Mouse CHAD shares 95% and 98% aa sequence identity with human and rat CHAD, respectively.

References