Recombinant Mouse Chordin-Like 2 Protein

Catalog #: 2520-CH Datasheet / COA / SDS

Carrier Free

Catalog #	Availability	Size / Price	Qty
2520-CH-050/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
2520-CH-050

R&D Systems Recombinant Proteins and Enzymes

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All Chordin-like 2/CHRDL2 Proteins and Enzymes

Product Details

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Chordin-Like 2 Protein Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED₅₀ for this effect is 0.2-1 µg/mL in the presence of 30 ng/mL of rhBMP-4.

Source

Mouse myeloma cell line, NS0-derived mouse Chordin-like 2/CHRDL2 protein
Gln24-Leu426, with a C-terminal 6-His tag

Accession #

Q8VEA6.1

N-terminal Sequence
Analysis

No results obtained: Gln24 predicted

Predicted Molecular Mass

46 kDa

SDS-PAGE

50-60 kDa, reducing conditions

Product Datasheets

2520-CH (with carrier)

Product Datasheet

COA

SDS

2520-CH/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2520-CH

Formulation	Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

2520-CH/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Chordin-like 2/CHRDL2

Chordin-like 2 (CHL2, CHRDL2, or BNF-1), is an approximately 55 kDa secreted glycoprotein that acts as a BMP antagonist. Mature mouse CHL2 contains three von Willebrand type C repeats and shares 73% and 95% amino acid sequence identity with human and rat CHL2, respectively (1-3). Alternative splicing of mouse Chordin-like 2 generates additional isoforms with substituted N-terminal or C-terminal regions (3). CHL2 is expressed in chondrocytes in developing joint cartilage (2, 4), osteoblasts, myotubes, brain, some carcinomas, and the genitourinary system (e.g. uterus, prostate, testis, bladder) (1-3). CHL2 binds to multiple TGF-beta superfamily proteins including BMP-2, -4, -5, -6, -7, GDF-5, Activin A, and TSG (2, 3, 5, 6). It prevents the binding of BMPs to type 1 and type 2 receptors as well as BMP-induced cellular responses (2, 5). CHL2 reduces the rate of matrix deposition by mesenchymal cells, acting as a negative regulator of cartilage formation (2).

References