Recombinant Mouse CHST4 Protein, CF
Recombinant Mouse CHST4 Protein, CF Summary
Product Specifications
Arg30-Gly388 with an N-terminal 6-His tag
Accession # Q9R1I1
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
5547-ST
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Assay Procedure
- Assay Buffer: 0.1 M Tris, 30 mM MgCl2, pH 7.5
- Recombinant Mouse Carbohydrate Sulfotransferase 4/CHST4 (rmCHST4) (Catalog # 5547-ST)
- Donor Substrate: 3′-Phosphoadenosine-5′-phosphosulfate/PAPS (PAPS) (Catalog # ES019)
- Acceptor Substrate: N-acetyl-alpha -D-glucosamine (GlcNAc) (Calbiochem, Catalog #1079), 1 M stock in deionized water
- Universal Sulfotransferase Activity Kit (Catalog # EA003)
- 96-well Clear Plate
- Plate Reader (Model: SpectraMax Plus by Molecular Devices) or equivalent
- Dilute 1 mM Phosphate Standard by adding 40 µL of the 1 mM Phosphate Standard to 360 µL of Assay Buffer for a 100 µM stock. This is the first point of the standard curve.
- Continue standard curve by performing six one-half serial dilutions of the 100 µM Phosphate stock in Assay Buffer. The standard curve has a range of 0.078 to 5.0 nmol per well.
- Prepare reaction mixture by containing 0.286 mM PAPS, 0.643 M GlcNAc, 0.0143 mg/mL Coupling Phosphatase 3.
- Dilute rmCHST4 to 40 µg/mL in Assay Buffer.
- Load 50 µL of each dilution of the standard curve into a plate. Include a curve blank containing 50 μL of Assay Buffer.
- Load 15 µL of the 40 µg/mL rmCHST4 into the plate. Include a Substrate Blank containing 15 µL of Assay Buffer.
- Add 35 µL of reaction mixture to the wells, excluding the standard curve.
- Cover the plate with a plate sealer and incubate at 37 °C for 20 minutes.
- Add 30 µL of the Malachite Green Reagent A to all wells. Mix briefly.
- Add 100 µL of deionized water to all wells. Mix briefly.
- Add 30 µL of the Malachite Green Reagent B to all wells. Mix and incubate for 20 minutes at room temperature.
- Read plate at 620 nm (absorbance) in endpoint mode.
- Calculate specific activity:
|
Specific Activity (pmol/min/µg) = |
Phosphate released* (nmol) x (1000 pmol/nmol) |
| Incubation time (min) x amount of enzyme (µg) |
*Derived from the phosphate standard curve using linear or 4-parameter fitting and adjusted for Control.
Per Reaction:- rmCHST4: 0.6 µg
- Coupling Phosphatase 3: 0.5 μg
- PAPS: 0.2 mM
- GlcNAc: 450 mM
Reconstitution Calculator
Background: Carbohydrate Sulfotransferase 4/CHST4
The mouse CHST family is comprised of 13 enzymes. All members of this family are Golgi-localized type II membrane proteins (1). Only the luminal and enzymatic domain is expressed in each of the recombinant CHST proteins. These enzymes transfer sulfate (i.e., sulfonate) onto the 6-O or 4-O positions of GalNAc, Gal and GlcNAc residues on glycoproteins, proteoglycans and glycolipids (2). This sulfation often creates specific epitopes that can be recognized by extracellular matrix proteins, cell surface receptors and viruses (3). Human CHST4, also known as high endothelial cell N-acetylglucosamine 6-O-sulfotransferase (HEC-GlcNAc6ST) or L‑selectin ligand sulfotransferase (LSST), catalyzes the transfer of sulfate to position 6 of non-reducing GlcNAc residues within mucin-associated glycans that ultimately serve as L‑selectin ligands (4). It has a catalytic preference for core 2-branched mucin-type O‑glycans, but also has activity toward core 3 type of O‑glycans (5). Mouse CHST4 shares 72% amino acid sequence identity with the human ortholog. The enzyme activity was measured using a phosphatase coupled method (6).
- deGraffenried, D. and Bertozzi, C.R. (2003) J. Biol. Chem. 278:40282.
- Hemmerich, S. and Rosen, S. (2000) Glycobiology 10:849.
- Bowman, K. G. and Bertozzi, C. R. (1999) Chem. Biol. 5:447.
- Bistrup, A. et al. (1999) J. Cell Biol. 145:899.
- Uchimura, K. et al. (2002) J. Biol. Chem. 277: 3979.
- Prather,B. et al. (2012) Anal. Biochem. 423:86.
Product Specific Notices
Glogos is a registered trademark of Stratagene.FAQs
No product specific FAQs exist for this product, however you may
View all Proteins and Enzyme FAQsReviews for Recombinant Mouse CHST4 Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Mouse CHST4 Protein, CF and earn rewards!
Have you used Recombinant Mouse CHST4 Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
