Recombinant Mouse CL-K1/COLEC11 Protein, CF

Catalog #: 4687-CL Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
4687-CL-050

R&D Systems Recombinant Proteins and Enzymes

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Recombinant Mouse CL-K1/COLEC11 Protein, CF Summary

Product Specifications

Purity

>85%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to bind alpha -L-Fucose. Keshi, H. et al. (2006) Microbiol. Immunol. 50(12):1001.

Source

Mouse myeloma cell line, NS0-derived mouse CL-K1/COLEC11 protein
Gln27-Leu272, with a C-terminal 6-His tag

Accession #

Q3SXB8

N-terminal Sequence
Analysis

No results obtained: Gln27 predicted

Predicted Molecular Mass

27 kDa

SDS-PAGE

33-38 kDa, reducing conditions

Product Datasheets

4687-CL

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4687-CL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: CL-K1/COLEC11

Collectins constitute a family of C-type lectins that recognize molecular patterns expressed on pathogens. Members of this glycoprotein family contain a N-terminal domain, a collagen-like domain, a neck region, and a C-terminal carbohydrate recognition domain (CRD). Collectins are typically secreted molecules, although CL-P1 is membrane bound and CL-L1 is found in the cytoplasm (1 - 3). Collectin kidney 1 (CL-K1), also known as collectin subfamily member 11 (COLEC11), is a 37 kDa collectin that circulates in the serum (4, 5). It associates into disulfide-linked oligomers and preferentially interacts with fucose residues in a calcium-dependent manner (4). Mature mouse CL-K1 shares 94% and 98% amino acid sequence identity with human and rat CL-K1, respectively. Within the CRD, mouse CL-K1 shares 52% aa sequence identity with CL-L1 and 23% - 32% aa sequence identity with collectins CL-P1, MBL, SP-A, and SP-D.

References

Gupta, G. and A. Surolia (2007) Bioessays 29:452.
van de Wetering, J.K. et al. (2004) Eur. J. Biochem. 271:1229.
Holmskov, U. et al. (2003) Annu. Rev. Immunol. 21:547.
Keshi, H. et al. (2006) Microbiol. Immunol. 50:1001.
Accession # NP_082142.

Long Name

Collectin Kidney 1

Entrez Gene IDs

78989 (Human); 71693 (Mouse)

Alternate Names

CLK1; CL-K1; CL-K1-I; CL-K1-II; CL-K1-IIa; CL-K1-IIb; COLEC11; collectin kidney I; Collectin kidney protein 1; collectin sub-family member 11; collectin-11; DKFZp686N1868; MGC3279