Recombinant Mouse CLEC4E Fc Chimera Protein, CF

Catalog #: 9238-CL Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9238-CL-050

Recombinant Mouse CLEC4E Fc Chimera Protein Bioactivity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse CLEC4E Fc Chimera Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Trehalose 6, 6'-Dimycolate is immobilized at 1 µg/mL (100 µL/well), the concentration of Recombinant Mouse CLEC4E Fc Chimera that produces 50% of the optimal binding response is approximately 8-48 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived mouse CLEC4E protein

MDP	Mouse IgG_2A (Glu98-Lys330)	IEGR	Mouse CLEC4E (Thr46-Asp214) Accession # Q9R0Q8
N-terminus			C-terminus

Accession #

Q9R0Q8

N-terminal Sequence
Analysis

Met

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

47 kDa

SDS-PAGE

54-63 kDa, reducing conditions

Product Datasheets

9238-CL

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9238-CL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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When Trehalose 6, 6'-Dimycolate is immobilized at 1 µg/mL (100 µL/well), Recombinant Mouse CLEC4E Fc Chimera (Catalog # 9238-CL) binds with an ED₅₀ of 8-48 ng/mL.

Reconstitution Calculator

Background: CLEC4E

CLEC4E, also known as Mincle, is an approximately 30 kDa type 2 transmembrane C-type lectin that functions as an activating innate immune receptor (1). Mouse CLEC4E consists of a 19 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 174 aa extracellular domain (ECD) that contains the C-type lectin domain (2). Within the ECD, mouse CLEC4E shares 65% and 87% aa sequence identity with human and rat CLEC4E, respectively. CLEC4E is expressed on monocytes, macrophages, and immature dendritic cells (2-5). It associates with CLEC4D/MCL and the gamma chain signaling subunits of Fc receptors (mediated by an Arg residue in the CLEC4E transmembrane segment) (3, 5, 6). Human CLEC4E binds to mycobacterial glycolipids including the immune adjuvant TDM (cord factor), its synthetic analog TDB, and GroMM (3, 4, 7-10). It also binds the nuclear protein SAP130 which can be released from necrotic cells (5) and cholesterol crystals deposited in atherosclerotic plaques (11). Mouse CLEC4E, in contrast, does not appear to interact with TDB, GroMM, or cholesterol crystals (7, 8, 11). CLEC4E ligation triggers phagocytosis and the production of inflammatory chemokines and cytokines (3-6, 8, 10). The fungus Fonsecaea monophora may evade immune clearance through binding to CLEC4E and suppressing IL-12 production and Th1 cell differentiation instead of promoting inflammation (9).

References

Richardson, M.B. and S.J. Williams (2014) Front. Immunol. 5:288.
Matsumoto, M. et al. (1999) J. Immunol. 163:5039.
Ishikawa, E. et al. (2009) J. Exp. Med. 206:2879.
Wells, C.A. et al. (2008) J. Immunol. 180:7404.
Yamasaki, S. et al. (2008) Nat. Immunol. 9:1179.
Lobato-Pascual, A. et al. (2013) Eur. J. Immunol. 43:3167.
Hattori, Y. et al. (2014) J. Biol. Chem. 289:15405.
Ostrop, J. et al. (2015) J. Immunol. 195:2417.
Wevers, B.A. et al. (2014) Cell Host Microbe 15:494.
Ishikawa, T. et al. (2013) Cell Host Microbe 13:477.
Kiyotake, R. et al. (2015) J. Biol. Chem. 290:25322.

Long Name

C-type Lectin Domain Family 4, Member E

Entrez Gene IDs

26253 (Human); 56619 (Mouse); 450223 (Rat)

Alternate Names

CLEC4E; CLECSF9; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 9; C-type lectin domain family 4 member E; C-type lectin domain family 4, member E; Macrophage-inducible C-type lectin; MINCLE; MINCLEC-type lectin superfamily member 9