Recombinant Mouse CLEC4F/CLECSF13 Protein, CF

Catalog #: 2784-CL Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
2784-CL-050

R&D Systems Recombinant Proteins and Enzymes

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Recombinant Mouse CLEC4F/CLECSF13 Protein, CF Summary

Product Specifications

Purity

>85%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to bind fluorescein-conjugated S. aureus Bioparticles. The ED₅₀ for this effect is 0.75-3.75 µg/mL.

Source

Mouse myeloma cell line, NS0-derived mouse CLEC4F/CLECSF13 protein
Ala65-Gly548, with an N-terminal 9-His tag

Accession #

P70194

N-terminal Sequence
Analysis

His

Predicted Molecular Mass

55 kDa

SDS-PAGE

65-85 kDa, reducing conditions

Product Datasheets

2784-CL

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2784-CL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: CLEC4F/CLECSF13

CLEC4F (C-type lectin domain; family 4, member F; also known as the Kupffer cell receptor and fucose receptor) is an 80 kDa, type II transmembrane glycoprotein member of the C-type lectin superfamily (1 - 3). Mature mouse CLEC4F consists of a 42 amino acid (aa) cytoplasmic domain, a 27 aa transmembrane segment, and a 479 aa extracellular domain (ECD) that contains an extended stalk region plus one carbohydrate recognition domain (4, 5). Within the ECD, mouse CLEC4F shares 48% and 79% aa sequence identity with human and rat CLEC4F, respectively. The stalk region of CLEC4F is a coiled coil domain that mediates homotrimer formation (6, 7). CLEC4F is expressed on Kupffer cells in the liver, but not on macrophages in other tissues (8). CLEC4F preferentially binds galactose and N-acetylgalactosamine in a calcium-dependent manner (6, 9, 10). Its activity at neutral, but not at acidic pH, suggests a capacity to internalize and release ligands into the endosomal system (11).

References

Zelensky, A.N. and J.E. Gready (2005) FEBS J. 272:6179.
Bilzer, M. et al. (2006) Liver Int. 26:1175.
Kuiper, J. et al. (1994) Biochem. J. 299:285.
Accession # P70194.
Hoyle, G.W. and R.L. Hill (1988) J. Biol. Chem. 263:7487.
Fadden, A.J. et al. (2003) Glycobiology 13:529.
Beavil, A.J. et al. (1992) Proc. Natl. Acad. Sci. 89:753.
Haltiwanger, R.S. et al. (1986) J. Biol. Chem. 261:7433.
Coombs, P.J. et al. (2006) Glycobiology 16:1C.
Biessen, E.A.L. et al. (1994) Biochem. J. 299:291.
Lehrman, M.A. et al. (1986) J. Biol. Chem. 261:7426.

Long Name

C-type Lectin Domain Family 4, Member F

Entrez Gene IDs

165530 (Human); 51811 (Mouse); 114598 (Rat)

Alternate Names

CLEC4F; CLECSF13; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 13; C-type lectin 13; C-type lectin domain family 4, member F; Galactose Particle Receptor; KCLR; KCR