Recombinant Mouse Contactin-2/TAG1 Protein, CF

Catalog #: 4439-CN Datasheet / COA / SDS

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4439-CN-050

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Contactin-2/TAG1 Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Able to significantly enhance neurite outgrowth when immobilized at 6-25 μg/mL on a nitrocellulose-coated microplate.

Source

Mouse myeloma cell line, NS0-derived mouse Contactin-2/TAG1 protein
Gln31-Ser1014, with a C-terminal 6-His tag

Accession #

Q61330

N-terminal Sequence
Analysis

No results obtained: Gln31 predicted

Predicted Molecular Mass

108.1 Kda

SDS-PAGE

135-145 kDa, under reducing conditions

Product Datasheets

4439-CN

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4439-CN

Formulation	Supplied as a 0.2 μm filtered solution in PBS.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after opening.

Reconstitution Calculator

Background: Contactin-2/TAG1

Contactin-2 (CNTN2), also called TAG1 (transient axonal glycoprotein), TAX1 (transiently-expressed axonal glycoprotein), or axonin-1, is a 135 kDa glycosyl-phosphatidylinositol (GPI)- anchored cell adhesion molecule that belongs to the contactin subfamily within the immunoglobulin (Ig) protein superfamily (1 - 3). Mouse Contactin-2 cDNA encodes a 30 amino acid (aa) signal peptide, a 984 aa mature secreted protein with 6 Ig-like domains followed by 4 fibronectin type III-like repeats, and a 26 aa C-terminal GPI anchor pro-sequence. GPI-specific phospholipase activity can release soluble, active Contactin-2 from the membrane (2). Mature mouse Contactin-2 shares approximately 93%, 97% and 77% aa sequence identity with human, rat and chicken Contactin-2, respectively. During development, Contactin-2 is expressed by a subset of neuronal populations in the central nervous system (CNS) and peripheral nervous system (PNS), particularly during initial phases of axon outgrowth (3 - 5). Both the 135 kDa form and a 90 kDa form are also upregulated in response to CNS injury in the adult (6). Data support a role for Contactin-2 in axon pathfinding, neurite outgrowth and adhesion, especially in the CNS (3 - 6). In mature myelinated fibers, Contactin-2 is expressed by oligodendrocytes and Schwann cells, which are myelinating glial cells of the CNS and PNS, respectively (7, 8). It is enriched in the juxtaparanodal regions, where it recruits caspr2 (contactin-associated protein 2), a transmembrane neurexin involved in cell adhesion and intercellular communication (7 - 10). The axonal Contactin-2 interacts in cis with caspr2, and in trans with another Contactin-2 on the glial membrane (8). This ternary complex is required for the accumulation and organization of K+ channels in the juxtaparanodes (9).

References

Wolfer, D. and R. J. Giger (1994) Swissprot Accession # Q61330.
Hasler, T.H. et al. (1993) Eur. J. Biochem. 211:329.
Karagogeos, D. (2003) Front. Biosci. 8:s1304.
Liu, Y. and M.C. Halloran (2005) J. Neurosci. 25:10556.
Denaxa, M. et al. (2005) Dev. Biol. 288:87.
Soares, S. et al. (2005) Eur. J. Neurosci. 21:1169.
Traka, M. et al. (2002) J. Neurosci. 22:3016.
Poliak, S. and E. Peles (2003) Nat. Rev. Neurosci. 4:968.
Traka, M. et al. (2003) J. Cell Biol. 162:1161.
Poliak, S. et al. (2003) J. Cell Biol. 162:1149.

Entrez Gene IDs

6900 (Human); 21367 (Mouse)

Alternate Names

Axonal glycoprotein TAG-1; axonin-1 cell adhesion molecule; Axonin-1; AXT; CNTN2; contactin 2 (axonal); contactin 2 (transiently expressed); Contactin2; Contactin-2; DKFZp781D102; FLJ37193; MGC157722; TAG1; TAG-1; TAX; TAX1; TAX-1; TAX1FLJ42746; Transient axonal glycoprotein 1; transiently-expressed axonal glycoprotein