Recombinant Mouse CRIM1 Protein, CF

Catalog #: 6050-CM Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
6050-CM-050

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse CRIM1 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by the ability of the immobilized protein to support the adhesion of NIH‑3T3 mouse embryonic fibroblast cells. Glienke, J. et al. (2002) Mech. Dev. 119:165.

rmCRIM1 immobilized at 3 μg/mL, 100 μL/well, will mediate >50% NIH3T3 cell adhesion (added at 1 x 10⁵ cells/well).

Source

Mouse myeloma cell line, NS0-derived mouse CRIM1 protein
Leu35-Ser940, with a C-terminal 6-His tag

Accession #

Q9JLL0

N-terminal Sequence
Analysis

Leu35

Structure / Form

Noncovalently-linked oligomer

Predicted Molecular Mass

100.3 kDa (monomer)

SDS-PAGE

120-130 kDa, reducing conditions

Product Datasheets

6050-CM

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6050-CM

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: CRIM1

CRIM1 (cysteine-rich motor neuron 1) is a type I transmembrane glycoprotein of the chordin-like cysteine-rich repeat (CRR) family of BMP inhibitors (1 - 4). The ~130 kDa, 1037 amino acid (aa) CRIM1 contains a 34 aa signal sequence, a 906 aa extracellular domain (ECD), a 21 aa transmembrane domain and a 76 aa cytoplasmic domain. The ECD includes an N-terminal IGF-binding protein-like motif and six chordin-like von Willebrand C-type CRRs. The ECD can be released from the cell, presumably by proteolytic processing (4). Mouse CRIM1 ECD shares 88%, 97%, 94%, 93% and 89% aa identity with human, rat, canine, equine and bovine CRIM1 ECD, respectively. CRIM1 CRR domains can interact with several cystine knot-containing growth factors, including VEGF-A, PDGF A and B, and BMPs 2, 4 and 7 (4, 5). It appears to bind VEGF or BMPs intracellularly, and antagonize them by lowering their expression, processing and secretion (4, 5). CRIM1 is expressed in the developing spinal cord in the floor plate and developing motor neurons (1, 6). It is also expressed by perivascular smooth muscle cells and aligns at points of cell-cell contact during endothelial cell capillary formation (2). Endothelial cell expression in vitro appears to be specific to cells that are adherent and growing (2). CRIM1 is also expressed in a spatially and temporally restricted manner in the developing lens, limbs, kidney, teeth and testis (6, 7). In adult kidney, it is expressed in glomeruli and the peritubular vasculature, where VEGF-A is constitutively expressed (5, 8). It is thought to regulate delivery of VEGF-A by podocytes to the kidney endothelium (5).

References

Kolle, G. et al. (2000), Mech. Dev. 90:181.
Glienke, J. et al. (2002) Mech. Dev. 119:165.
Abreu, J. G. et al. (2002) Gene 287:39.
Wilkinson, L. et al. (2003) J. Biol. Chem. 278:34181.
Wilkinson, L. et al. (2007) J. Am. Soc. Nephrol. 18:1697.
Kolle, G. et al. (2003) Dev. Dyn. 226:107.
Pennisi, D. J. et al. (2007) Dev. Dyn. 236:502.
Wilkinson, L. et al. (2009) Kidney Int. 76:1161.

Long Name

Cysteine Rich Motor Neuron 1 Protein

Entrez Gene IDs

51232 (Human); 50766 (Mouse)

Alternate Names

CRIM1; CRIM-1; cysteine rich transmembrane BMP regulator 1 (chordin-like); cysteine-rich motor neuron 1 protein; cysteine-rich motor neuron 1; Cysteine-rich repeat-containing protein S52; MGC138194; S52