Recombinant Mouse CXCL11/I-TAC Protein

Catalog #: 572-MC
4 Citations Datasheet / COA / SDS

Carrier Free

Catalog #	Availability	Size / Price	Qty
572-MC-025/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
572-MC-025

R&D Systems Recombinant Proteins and Enzymes

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Product Details

Citations (4)

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse CXCL11/I-TAC Protein Summary

Product Specifications

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR3. The ED₅₀ for this effect is 3‑15 ng/mL.

Source

E. coli-derived mouse CXCL11/I-TAC protein
Phe22-Met100

Accession #

Q9JHH5

N-terminal Sequence
Analysis

Phe22

Predicted Molecular Mass

9.1 kDa

Product Datasheets

572-MC (with carrier)

Product Datasheet

COA

SDS

572-MC/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

572-MC

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

572-MC/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: CXCL11/I-TAC

CXCL11, also known as I-TAC, SCYB9B, H174, IP-9, and beta -R1, is a member within the non-ELR CXC chemokine subgroup and has been designated CXCL11. CXCL11, together with MIG and IP-10, constitute a subset of chemokines that are ligands for CXCR3, a chemokine receptor that is primarily expressed on activated Th1 cells and NK cells. The three chemokines were also reported to act as antagonists for CCR3, a chemokine receptor that is preferentially expressed on activated Th2 cells. Mouse CXCL11 cDNA encodes a 100 amino acid (aa) residue precursor protein with a putative 21 aa residue signal peptide that is cleaved to yield a 79 aa residue mature protein. Mature mouse and human CXCL11 share 71% aa sequence identity. Mouse CXCL11 also shares 36% and 29% aa sequence identity with mouse IP-10 (Crg-2) and mouse MIG, respectively. The gene for mouse CXCL11 has been mapped to chromosome 5, in close proximity to the IP-10 and MIG genes. Mouse CXCL11 is induced in multiple tissues during endoxemia, with the greatest expression in lung, heart, small intestine and kidney. The endotoxemia-induced mouse CXCL11 expression is strongly attenuated by treatment with glucocorticoid.

References

Widney, D.P. et al. (2000) J. Immunol. 164:6322.
Meyer, M. et al. (2000) Cytogenet. Cell Genet. 88:278.
Loetscher, P. et al. (2001) J. Biol. Chem. Manuscript M005652200.

Entrez Gene IDs

6373 (Human); 56066 (Mouse)

Alternate Names

beta-R1; b-R1; chemokine (C-X-C motif) ligand 11; CXCL11; H174; H174IP9; Interferon gamma-inducible protein 9; Interferon-inducible T-cell alpha chemoattractant; IP-9member 11; ITAC; I-TAC; I-TACMGC102770; SCYB9B; small inducible cytokine subfamily B (Cys-X-Cys), member 9B; Small-inducible cytokine B11

Related Research Areas

Citations for Recombinant Mouse CXCL11/I-TAC Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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The atypical chemokine receptor 3 interacts with Connexin 43 inhibiting astrocytic gap junctional intercellular communication
Authors: A Fumagalli, J Heuninck, A Pizzoccaro, E Moutin, J Koenen, M Séveno, T Durroux, MP Junier, G Schlecht-L, F Bachelerie, D Schütz, R Stumm, MJ Smit, NC Guérineau, S Chaumont-D, P Marin
Nat Commun, 2020-09-25;11(1):4855.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
TGFbeta signaling in myeloid cells regulates mammary carcinoma cell invasion through fibroblast interactions.
Authors: Shaw A, Pickup M, Chytil A, Aakre M, Owens P, Moses H, Novitskiy S
PLoS ONE, 2015-01-28;10(1):e0117908.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
Early differentiated CD138(high) MHCII+ IgG+ plasma cells express CXCR3 and localize into inflamed kidneys of lupus mice.
Authors: Lacotte S, Decossas M, Le Coz C, Brun S, Muller S, Dumortier H
PLoS ONE, 2013-03-08;8(3):e58140.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
The role of CCL21 in recruitment of T-precursor cells to fetal thymi.
Authors: Ueno T, Kuse S, Saito F, Nitta T, Kakiuchi T, Hollander GA, Takahama Y
Blood, 2004-09-09;105(1):31-9.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay