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Recombinant Mouse CXCL14/BRAK Protein, CF

Catalog #: 730-XC
7 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
730-XC-025
R&D Systems Recombinant Proteins and Enzymes
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Citations (7)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse CXCL14/BRAK Protein, CF Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to bind fluorescein-conjugated E. coli Bioparticles. The ED50 for this effect is 2-8 μg/mL.
Source
E. coli-derived mouse CXCL14/BRAK protein
Ser23-Glu99
Accession #
Q9JHH7
N-terminal Sequence
Analysis
Ser23
Predicted Molecular Mass
9.4 kDa

Product Datasheets

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730-XC

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

730-XC

Formulation Supplied as a 0.2 μm filtered solution in PBS.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CXCL14/BRAK

CXCL14/BRAK, also named MIP-2 gamma, KEC (kidney-expressed chemokine), and BMAC (B cell and monocyte-activating chemokine), is a member of the CXC chemokine superfamily (1-5). The deduced 99 amino acid (aa) residue precursor has a 22 aa putative signal peptide that is cleaved to produce the 77 aa mature protein. Mature human and mouse CXCL14 differ by only 2 residues. Mouse CXCL14 shares approximately 30% aa sequence identity with mouse MIP‑2. Unlike MIP‑2, CXCL14 lacks the ELR domain preceding the CXC motif. CXCL14 transcripts are constitutively expressed at high levels in the basal layer of epidermal keratinocytes and dermal fibroblasts of skin tissues as well as lamina propria cells in normal intestinal tissues. CXCL14 has been shown to be a highly selective chemoattractant for monocytes that have been treated with prostaglandin E2 or forskolin, agents that activate adenylate cyclase. CXCL14 has been proposed to be important in regulating the trafficking of macrophage precursor to regions in skin and mucosal tissues that support their development. Consistent with this hypothesis, macrophages were frequently found to co-localize with CXCL14-producing cells in the dermis and lamina propria.

References
  1. Hromas, R. et al. (1999) Biochem. Biophys. Res. Commun. 255:703.
  2. Cao, X. et al. (2000) J. Immunol. 165:2588.
  3. Kurth, I. et al. (2001) J. Exp. Med. 194:855.
  4. Frederick, M.J. et al. (2000) Am. J. Pathol. 156:1937.
  5. Sleeman, M.A. et al. (2000) Int. Immunol. 12:677.
Entrez Gene IDs
9547 (Human); 57266 (Mouse)
Alternate Names
BMAC; Bolekine; BRAK; BRAKKEC; chemokine (C-X-C motif) ligand 14; Chemokine BRAK; CXCL14; CXC-X3; member 14 (BRAK); MIP-2 gamma; MIP2G; MIP-2G; NJACKec; SCYB14MGC10687; Small-inducible cytokine B14

Citations for Recombinant Mouse CXCL14/BRAK Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Geniposide ameliorates atherosclerosis by regulating macrophage polarization via perivascular adipocyte-derived CXCL14
    Authors: He, P;Wang, H;Cheng, S;Hu, F;Zhang, L;Chen, W;Xu, Y;Zhang, Y;Gu, Y;Li, Z;Jin, Y;Liu, X;Jia, Y;
    Journal of ethnopharmacology
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. FGF-2 signaling in nasopharyngeal carcinoma modulates pericyte-macrophage crosstalk and metastasis
    Authors: Y Wang, Q Sun, Y Ye, X Sun, S Xie, Y Zhan, J Song, X Fan, B Zhang, M Yang, L Lv, K Hosaka, Y Yang, G Nie
    JCI Insight, 2022-05-23;0(0):.
    Species: Mouse
    Sample Types:
    Applications: Bioassay
  3. Cancer-associated fibroblasts expressing CXCL14 rely upon NOS1-derived nitric oxide signaling for their tumor-supporting properties.
    Authors: Augsten M, Sjoberg E, Frings O, Vorrink S, Frijhoff J, Olsson E, Borg A, Ostman A
    Cancer Res, 2014-04-07;74(11):2999-3010.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. CXCL14 inhibits trophoblast outgrowth via a paracrine/autocrine manner during early pregnancy in mice.
    Authors: Kuang H, Chen Q, Fan X, Zhang Y, Zhang L, Peng H, Cao Y, Duan E
    J. Cell. Physiol., 2009-11-01;221(2):448-57.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. CXCL14 enhances insulin-dependent glucose uptake in adipocytes and is related to high-fat diet-induced obesity.
    Authors: Takahashi M, Takahashi Y, Takahashi K, Zolotaryov FN, Hong KS, Iida K, Okimura Y, Kaji H, Chihara K
    Biochem. Biophys. Res. Commun., 2007-10-29;364(4):1037-42.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Novel regulators of kidney development from the tips of the ureteric bud.
    Authors: Schmidt-Ott KM, Yang J, Chen X, Wang H, Paragas N, Mori K, Li JY, Lu B, Costantini F, Schiffer M, Bottinger E, Barasch J
    J. Am. Soc. Nephrol., 2005-05-25;16(7):1993-2002.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: Bioassay
  7. The role of CCL21 in recruitment of T-precursor cells to fetal thymi.
    Authors: Ueno T, Kuse S, Saito F, Nitta T, Kakiuchi T, Hollander GA, Takahama Y
    Blood, 2004-09-09;105(1):31-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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