Recombinant Mouse CXCL2/MIP-2 Protein Summary
Product Specifications
Ala28-Asn100
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
452-M2
Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. |
Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
452-M2/CF
Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. |
Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: CXCL2/GRO beta/MIP-2/CINC-3
Macrophage Inflammatory Protein-2 (MIP-2) was originally identified as a heparin-binding protein secreted from a murine macrophage cell line in response to endotoxin stimulation. Based on its protein and DNA sequences, MIP-2 is a member of the alpha (C-X-C) subfamily of chemokines.
MIP-2 cDNA encodes a 100 amino acid residue precursor protein from which the amino-terminal 27 amino acid residues are cleaved to generate the mature MIP-2. The protein sequence of murine MIP-2 shows approximately 63% identity to that of murine KC, another murine alpha chemokine whose expression is induced by PDGF. In addition, the protein sequence of MIP-2 is also 60% identical to human GRO beta and GRO gamma. It has been suggested that mouse KC and MIP-2 are the homologs of the human GROs and rat CINCs.
Similarly to other alpha chemokines, murine MIP-2 is a potent neutrophil attractant and activator. MIP-2 and KC can bind the murine interleukin 8 type B receptor homologue with high affinity. The expression of MIP-2 was found to be associated with neutrophil influx in pulmonary inflammation and glomerulonephritis, suggesting that MIP-2 may contribute to the pathogenesis of inflammatory diseases.
Citations for Recombinant Mouse CXCL2/MIP-2 Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Interleukin-9 promotes mast cell progenitor proliferation and CCR2-dependent mast cell migration in allergic airway inflammation
Authors: Pajulas, A;Fu, Y;Cheung, CCL;Chu, M;Cannon, A;Alakhras, N;Zhang, J;Ulrich, BJ;Nelson, AS;Zhou, B;Kaplan, MH;
Mucosal immunology
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Senescent hepatic stellate cells promote liver regeneration through IL-6 and ligands of CXCR2
Authors: N Cheng, KH Kim, LF Lau
JCI Insight, 2022-06-16;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
DEK, a nuclear protein, is chemotactic for hematopoietic stem/progenitor cells acting through CXCR2 and Galphai signaling
Authors: ML Capitano, Y Sammour, J Ropa, M Legendre, N Mor-Vaknin, DM Markovitz
Journal of leukocyte biology, 2022-02-08;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Programmed 'disarming' of the neutrophil proteome reduces the magnitude of inflammation
Authors: JM Adrover, A Aroca-Crev, G Crainiciuc, F Ostos, Y Rojas-Vega, A Rubio-Ponc, C Cilloniz, E Bonzón-Kul, E Calvo, D Rico, MA Moro, C Weber, I Lizasoaín, A Torres, J Ruiz-Cabel, J Vázquez, A Hidalgo
Nat. Immunol., 2020-01-13;21(2):135-144.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Chemokine-Binding Proteins Encoded by Parapoxvirus of Red Deer of New Zealand Display Evidence of Gene Duplication and Divergence of Ligand Specificity
Authors: S Sharif, N Ueda, Y Nakatani, LM Wise, S Clifton, Z Lateef, AA Mercer, SB Fleming
Front Microbiol, 2019-06-25;10(0):1421.
Species: Mouse
Sample Types:
Applications: Binding Assay -
Critical role for PI3K?-dependent neutrophil reactive oxygen species in WKYMVm-induced microvascular hyperpermeability
Authors: L Hao, X Lei, H Zhou, AJ Marshall, L Liu
J. Leukoc. Biol., 2019-06-19;0(0):.
Species: Mouse, Transgenic Mouse
Sample Types: In Vivo
Applications: In Vivo -
Secreted nuclear protein DEK regulates hematopoiesis through CXCR2 signaling
Authors: ML Capitano, N Mor-Vaknin, AK Saha, S Cooper, M Legendre, H Guo, R Contreras-, F Kappes, MA Sartor, CT Lee, X Huang, DM Markovitz, HE Broxmeyer
J. Clin. Invest., 2019-05-20;130(0):2555-2570.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Ferroptotic cell death and TLR4/Trif signaling initiate neutrophil recruitment after heart transplantation
Authors: W Li, G Feng, JM Gauthier, I Lokshina, R Higashikub, S Evans, X Liu, A Hassan, S Tanaka, M Cicka, HM Hsiao, D Ruiz-Perez, A Bredemeyer, RW Gross, DL Mann, YY Tyurina, AE Gelman, VE Kagan, A Linkermann, KJ Lavine, D Kreisel
J. Clin. Invest., 2019-02-26;130(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Induced Pluripotent Stem Cells Regulate TREM-1 Expression and the p38 MAPK Pathway in Endotoxin-Induced Acute Lung Injury
Authors: VY Su, KY Yang, SH Chiou, NJ Chen, MH Mo, CS Lin, CT Wang
Stem Cells, 2019-02-16;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
The Specific Mitogen- and Stress-Activated Protein Kinase MSK1 Inhibitor SB-747651A Modulates Chemokine-Induced Neutrophil Recruitment
Authors: M Hossain, E Omran, N Xu, L Liu
Int J Mol Sci, 2017-10-17;18(10):.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Malondialdehyde epitopes are sterile mediators of hepatic inflammation in hypercholesterolemic mice
Authors: Clara Jana-Lui Busch
Hepatology, 2017-02-03;0(0):.
Applications: ELISA (Standard) -
Main pathways of action of Brazilian red propolis on the modulation of neutrophils migration in the inflammatory process
Authors: Pedro Luiz Rosalen
Phytomedicine, 2016-09-28;23(13):1583-1590.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Endothelial LSP1 Modulates Extravascular Neutrophil Chemotaxis by Regulating Nonhematopoietic Vascular PECAM-1 Expression.
Authors: Hossain M, Qadri S, Xu N, Su Y, Cayabyab F, Heit B, Liu L
J Immunol, 2015-08-03;195(5):2408-16.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
DAP12 expression in lung macrophages mediates ischemia/reperfusion injury by promoting neutrophil extravasation.
Authors: Spahn J, Li W, Bribriesco A, Liu J, Shen H, Ibricevic A, Pan J, Zinselmeyer B, Brody S, Goldstein D, Krupnick A, Gelman A, Miller M, Kreisel D
J Immunol, 2015-03-11;194(8):4039-48.
Species: Mouse
Sample Types: In Vivo, Whole Cells
Applications: Bioassay, In Vivo -
Mutation in the CD45 inhibitory wedge modulates integrin activation and leukocyte recruitment during inflammation.
Authors: Germena G, Volmering S, Sohlbach C, Zarbock A
J Immunol, 2014-12-10;194(2):728-38.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Interference with glycosaminoglycan-chemokine interactions with a probe to alter leukocyte recruitment and inflammation in vivo.
Authors: Li S, Pettersson U, Hoorelbeke B, Kolaczkowska E, Schelfhout K, Martens E, Kubes P, Van Damme J, Phillipson M, Opdenakker G
PLoS ONE, 2014-08-05;9(8):e104107.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
MyD88-, but not Nod1- and/or Nod2-deficient mice, show increased susceptibility to polymicrobial sepsis due to impaired local inflammatory response.
Authors: Sonego F, Castanheira F, Czaikoski P, Kanashiro A, Souto F, Franca R, Nascimento D, Freitas A, Spiller F, Cunha L, Zamboni D, Alves-Filho J, Cunha F
PLoS ONE, 2014-08-01;9(8):e103734.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Macrophage-inducing FasL on chondrocytes forms immune privilege in cartilage tissue engineering, enhancing in vivo regeneration.
Authors: Fujihara Y, Takato T, Hoshi K
Stem Cells, 2014-05-01;32(5):1208-19.
Species: Human, Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Fibrates ameliorate the course of bacterial sepsis by promoting neutrophil recruitment via CXCR2.
Authors: Tancevski I, Nairz M, Duwensee K, Auer K, Schroll A, Heim C, Feistritzer C, Hoefer J, Gerner R, Moschen A, Heller I, Pallweber P, Li X, Theurl M, Demetz E, Wolf A, Wolf D, Eller P, Ritsch A, Weiss G
EMBO Mol Med, 2014-04-06;6(6):810-20.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
G protein-coupled receptor kinase 6 deficiency promotes angiogenesis, tumor progression, and metastasis.
Authors: Raghuwanshi S, Smith N, Rivers E, Thomas A, Sutton N, Hu Y, Mukhopadhyay S, Chen X, Leung T, Richardson R
J Immunol, 2013-04-15;190(10):5329-36.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: ELISA (Standard) -
Inefficient antimicrobial functions of innate phagocytes render infant mice more susceptible to bacterial infection.
Authors: Zhang Q, Coveney A, Yu S, Liu J, Li Y, Blankson S, Redmond H, Wang J, Wang J
Eur J Immunol, 2013-03-22;43(5):1322-32.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
alpha-Acid glycoprotein decreases neutrophil migration and increases susceptibility to sepsis in diabetic mice.
Authors: Spiller F, Carlos D, Souto FO, de Freitas A, Soares FS, Vieira SM, Paula FJ, Alves-Filho JC, Cunha FQ
Diabetes, 2012-03-13;61(6):1584-91.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
IL-17RA signaling amplifies antibody-induced arthritis.
Authors: Sadik CD, Kim ND, Alekseeva E, Luster AD
PLoS ONE, 2011-10-20;6(10):e26342.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Diesel exhaust particulates exacerbate asthma-like inflammation by increasing CXC chemokines.
Authors: Kim J, Natarajan S, Vaickus LJ, Bouchard JC, Beal D, Cruikshank WW, Remick DG
Am. J. Pathol., 2011-10-01;179(6):2730-9.
Applications: ELISA (Standard) -
Proinflammatory clearance of apoptotic neutrophils induces an IL-12(low)IL-10(high) regulatory phenotype in macrophages.
Authors: Filardy AA, Pires DR, Nunes MP, Takiya CM, Freire-de-Lima CG, Ribeiro-Gomes FL, DosReis GA
J. Immunol., 2010-07-21;185(4):2044-50.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
A chemotactic gradient sequestered on endothelial heparan sulfate induces directional intraluminal crawling of neutrophils.
Authors: Massena S, Christoffersson G, Hjertstrom E, Zcharia E, Vlodavsky I, Ausmees N, Rolny C, Li JP, Phillipson M
Blood, 2010-06-08;116(11):1924-31.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Prostaglandin mediates IL-23/IL-17-induced neutrophil migration in inflammation by inhibiting IL-12 and IFNgamma production.
Authors: Lemos HP, Grespan R, Vieira SM, Cunha TM, Verri WA, Fernandes KS, Souto FO, McInnes IB, Ferreira SH, Liew FY, Cunha FQ
Proc. Natl. Acad. Sci. U.S.A., 2009-03-16;106(14):5954-9.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
ST2 deficient mice display a normal host defense against pulmonary infection with Mycobacterium tuberculosis.
Authors: Wieland CW, van der Windt GJ, Florquin S, McKenzie AN, van der Poll T
Microbes Infect., 2009-03-13;11(4):524-30.
Applications: ELISA (Standard) -
CD14 contributes to pulmonary inflammation and mortality during murine tuberculosis.
Authors: Wieland CW, van der Windt GJ, Wiersinga WJ, Florquin S, van der Poll T
Immunology, 2008-04-03;125(2):272-9.
Applications: ELISA (Standard) -
Bv8 regulates myeloid-cell-dependent tumour angiogenesis.
Authors: Shojaei F, Wu X, Zhong C, Yu L, Liang XH, Yao J, Blanchard D, Bais C, Peale FV, van Bruggen N, Ho C, Ross J, Tan M, Carano RA, Meng YG, Ferrara N
Nature, 2007-12-06;450(7171):825-31.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
P-selectin glycoprotein ligand-1 regulates chemokine-dependent leukocyte recruitment in colonic ischemia-reperfusion.
Authors: Santen S, Schramm R, Menger MD, Wang Y, Jeppsson B, Thorlacius H
Inflamm. Res., 2007-11-01;56(11):452-8.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Mutations in the cofilin partner Aip1/Wdr1 cause autoinflammatory disease and macrothrombocytopenia.
Authors: Kile BT, Panopoulos AD, Stirzaker RA, Hacking DF, Tahtamouni LH, Willson TA, Mielke LA, Henley KJ, Zhang JG, Wicks IP, Stevenson WS, Nurden P, Watowich SS, Justice MJ
Blood, 2007-05-21;110(7):2371-80.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Leukocyte PI3Kgamma and PI3Kdelta have temporally distinct roles for leukocyte recruitment in vivo.
Authors: Liu L, Puri KD, Penninger JM, Kubes P
Blood, 2007-05-08;110(4):1191-8.
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay -
Normal neutrophil functions in sphingosine kinase type 1 and 2 knockout mice.
Authors: Zemann B, Urtz N, Reuschel R, Mechtcheriakova D, Bornancin F, Badegruber R, Baumruker T, Billich A
Immunol. Lett., 2007-01-30;109(1):56-63.
Species: Mouse
Sample Types: In Vivo, Whole Cells
Applications: Bioassay, In Vivo -
Murine CXCR1 is a functional receptor for GCP-2/CXCL6 and interleukin-8/CXCL8.
Authors: Fan X, Patera AC, Pong-Kennedy A, Deno G, Gonsiorek W, Manfra DJ, Vassileva G, Zeng M, Jackson C, Sullivan L, Sharif-Rodriguez W, Opdenakker G, Van Damme J, Hedrick JA, Lundell D, Lira SA, Hipkin RW
J. Biol. Chem., 2006-12-29;282(16):11658-66.
Species: Mouse
Sample Types: Complex Sample Type
Applications: Binding Assay -
Overexpression of suppressor of cytokine signaling-5 in T cells augments innate immunity during septic peritonitis.
Authors: Watanabe H, Kubo M, Numata K, Takagi K, Mizuta H, Okada S, Ito T, Matsukawa A
J. Immunol., 2006-12-15;177(12):8650-7.
Applications: ELISA (Standard) -
Lack of in vitro and in vivo recognition of Francisella tularensis subspecies lipopolysaccharide by Toll-like receptors.
Authors: Hajjar AM, Harvey MD, Shaffer SA, Goodlett DR, Sjostedt A, Edebro H, Forsman M, Bystrom M, Pelletier M, Wilson CB, Miller SI, Skerrett SJ, Ernst RK
Infect. Immun., 2006-09-18;74(12):6730-8.
Applications: ELISA (Standard) -
STAT3 governs distinct pathways in emergency granulopoiesis and mature neutrophils.
Authors: Panopoulos AD, Zhang L, Snow JW, Jones DM, Smith AM, El Kasmi KC, Liu F, Goldsmith MA, Link DC, Murray PJ, Watowich SS
Blood, 2006-08-03;108(12):3682-90.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
Authors: Osuchowski MF, Welch K, Siddiqui J, Remick DG
J. Immunol., 2006-08-01;177(3):1967-74.
Applications: ELISA (Standard) -
Neutrophil recruitment in immunized mice depends on MIP-2 inducing the sequential release of MIP-1alpha, TNF-alpha and LTB(4).
Authors: Ramos CD, Fernandes KS, Canetti C, Teixeira MM, Silva JS, Cunha FQ
Eur. J. Immunol., 2006-08-01;36(8):2025-34.
Applications: ELISA (Standard) -
Adenoviral-mediated overexpression of SOCS3 enhances IgG immune complex-induced acute lung injury.
Authors: Gao H, Hoesel LM, Guo RF, Rancilio NJ, Sarma JV, Ward PA
J. Immunol., 2006-07-01;177(1):612-20.
Applications: ELISA (Standard) -
The injury response of oligodendrocyte precursor cells is induced by platelets, macrophages and inflammation-associated cytokines.
Authors: Rhodes KE, Raivich G, Fawcett JW
Neuroscience, 2006-04-21;140(1):87-100.
Species: Rat
Sample Types: In Vivo
Applications: In Vivo -
High expression of ligands for chemokine receptor CXCR2 in alveolar epithelial neoplasia induced by oncogenic kras.
Authors: Wislez M, Fujimoto N, Izzo JG, Hanna AE, Cody DD, Langley RR, Tang H, Burdick MD, Sato M, Minna JD, Mao L, Wistuba I, Strieter RM, Kurie JM
Cancer Res., 2006-04-15;66(8):4198-207.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Human CXCR2 (hCXCR2) takes over functionalities of its murine homolog in hCXCR2 knockin mice.
Authors: Mihara K, Smit MJ, Krajnc-Franken M, Gossen J, Rooseboom M, Dokter W
Eur. J. Immunol., 2005-09-01;35(9):2573-82.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Sequential ELISA to profile multiple cytokines from small volumes.
Authors: Osuchowski MF, Siddiqui J, Copeland S, Remick DG
J. Immunol. Methods, 2005-07-01;302(1):172-81.
Applications: ELISA (Standard) -
Transcriptional profiling of lipopolysaccharide-induced acute lung injury.
Authors: Jeyaseelan S, Chu HW, Young SK, Worthen GS
Infect. Immun., 2004-12-01;72(12):7247-56.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: ELISA Development -
The class A macrophage scavenger receptor attenuates CXC chemokine production and the early infiltration of neutrophils in sterile peritonitis.
Authors: Cotena A, Platt N
J. Immunol., 2004-11-15;173(10):6427-32.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
The role of CCL21 in recruitment of T-precursor cells to fetal thymi.
Authors: Ueno T, Kuse S, Saito F, Nitta T, Kakiuchi T, Hollander GA, Takahama Y
Blood, 2004-09-09;105(1):31-9.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Neutrophil-mediated mycobacteriocidal immunity in the lung during Mycobacterium bovis BCG infection in C57BL/6 mice.
Authors: Fulton SA, Reba SM, Martin TD, Boom WH
Infect. Immun., 2002-09-01;70(9):5322-7.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo
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View all Proteins and Enzyme FAQsReviews for Recombinant Mouse CXCL2/MIP-2 Protein
Average Rating: 4.3 (Based on 3 Reviews)
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Reason for Rating: Worked well in in vitro chemotaxis assay.
A total of 10^6 mouse neutrophils was added to an insert of a transwell. Recombinant mouse MIP-2 (100 ng/ml) was used as a neutrophil chemoattractant and was added to the lower chamber. After 2 hours of incubation, migrated neutrophils in the lower chambers were enumerated.