Recombinant Mouse CXCL2/MIP-2 Protein

Carrier Free

Catalog # Availability Size / Price Qty
452-M2-010/CF
452-M2-050/CF

With Carrier

Catalog # Availability Size / Price Qty
452-M2-010
452-M2-050
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (49)
FAQs
Reviews (3)

Recombinant Mouse CXCL2/MIP-2 Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR2. The ED50 for this effect is 0.500‑5.00 ng/mL.
Source
E. coli-derived mouse CXCL2/GRO beta/MIP-2/CINC-3 protein
Ala28-Asn100
Accession #
N-terminal Sequence
Analysis
Ala28
Predicted Molecular Mass
8 kDa

Product Datasheets

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452-M2 (with carrier)

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452-M2/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

452-M2

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

452-M2/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: CXCL2/GRO beta/MIP-2/CINC-3

Macrophage Inflammatory Protein-2 (MIP-2) was originally identified as a heparin-binding protein secreted from a murine macrophage cell line in response to endotoxin stimulation. Based on its protein and DNA sequences, MIP-2 is a member of the alpha (C-X-C) subfamily of chemokines.

MIP-2 cDNA encodes a 100 amino acid residue precursor protein from which the amino-terminal 27 amino acid residues are cleaved to generate the mature MIP-2. The protein sequence of murine MIP-2 shows approximately 63% identity to that of murine KC, another murine alpha chemokine whose expression is induced by PDGF. In addition, the protein sequence of MIP-2 is also 60% identical to human GRO beta and GRO gamma. It has been suggested that mouse KC and MIP-2 are the homologs of the human GROs and rat CINCs.

Similarly to other alpha chemokines, murine MIP-2 is a potent neutrophil attractant and activator. MIP-2 and KC can bind the murine interleukin 8 type B receptor homologue with high affinity. The expression of MIP-2 was found to be associated with neutrophil influx in pulmonary inflammation and glomerulonephritis, suggesting that MIP-2 may contribute to the pathogenesis of inflammatory diseases.

Entrez Gene IDs
2920 (Human); 20310 (Mouse); 114105 (Rat)
Alternate Names
chemokine (C-X-C motif) ligand 2; CINC-2a; CINC3; CINC-3; C-X-C Motif Chemokine 2; CXCL2; GRO beta; GRO2 oncogene; GRO2; GROB; Gro-beta; Growth-regulated protein beta; Macrophage inflammatory protein 2-alpha; melanoma growth stimulatory activity beta; MGSA beta; MGSA-b; MGSA-beta; MIP2; MIP-2; MIP2A; MIP-2a; MIP2-alpha; SCYB2

Citations for Recombinant Mouse CXCL2/MIP-2 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

49 Citations: Showing 1 - 10
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  1. Interleukin-9 promotes mast cell progenitor proliferation and CCR2-dependent mast cell migration in allergic airway inflammation
    Authors: Pajulas, A;Fu, Y;Cheung, CCL;Chu, M;Cannon, A;Alakhras, N;Zhang, J;Ulrich, BJ;Nelson, AS;Zhou, B;Kaplan, MH;
    Mucosal immunology
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Senescent hepatic stellate cells promote liver regeneration through IL-6 and ligands of CXCR2
    Authors: N Cheng, KH Kim, LF Lau
    JCI Insight, 2022-06-16;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  3. DEK, a nuclear protein, is chemotactic for hematopoietic stem/progenitor cells acting through CXCR2 and Galphai signaling
    Authors: ML Capitano, Y Sammour, J Ropa, M Legendre, N Mor-Vaknin, DM Markovitz
    Journal of leukocyte biology, 2022-02-08;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Programmed 'disarming' of the neutrophil proteome reduces the magnitude of inflammation
    Authors: JM Adrover, A Aroca-Crev, G Crainiciuc, F Ostos, Y Rojas-Vega, A Rubio-Ponc, C Cilloniz, E Bonzón-Kul, E Calvo, D Rico, MA Moro, C Weber, I Lizasoaín, A Torres, J Ruiz-Cabel, J Vázquez, A Hidalgo
    Nat. Immunol., 2020-01-13;21(2):135-144.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Chemokine-Binding Proteins Encoded by Parapoxvirus of Red Deer of New Zealand Display Evidence of Gene Duplication and Divergence of Ligand Specificity
    Authors: S Sharif, N Ueda, Y Nakatani, LM Wise, S Clifton, Z Lateef, AA Mercer, SB Fleming
    Front Microbiol, 2019-06-25;10(0):1421.
    Species: Mouse
    Sample Types:
    Applications: Binding Assay
  6. Critical role for PI3K?-dependent neutrophil reactive oxygen species in WKYMVm-induced microvascular hyperpermeability
    Authors: L Hao, X Lei, H Zhou, AJ Marshall, L Liu
    J. Leukoc. Biol., 2019-06-19;0(0):.
    Species: Mouse, Transgenic Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  7. Secreted nuclear protein DEK regulates hematopoiesis through CXCR2 signaling
    Authors: ML Capitano, N Mor-Vaknin, AK Saha, S Cooper, M Legendre, H Guo, R Contreras-, F Kappes, MA Sartor, CT Lee, X Huang, DM Markovitz, HE Broxmeyer
    J. Clin. Invest., 2019-05-20;130(0):2555-2570.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Ferroptotic cell death and TLR4/Trif signaling initiate neutrophil recruitment after heart transplantation
    Authors: W Li, G Feng, JM Gauthier, I Lokshina, R Higashikub, S Evans, X Liu, A Hassan, S Tanaka, M Cicka, HM Hsiao, D Ruiz-Perez, A Bredemeyer, RW Gross, DL Mann, YY Tyurina, AE Gelman, VE Kagan, A Linkermann, KJ Lavine, D Kreisel
    J. Clin. Invest., 2019-02-26;130(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Induced Pluripotent Stem Cells Regulate TREM-1 Expression and the p38 MAPK Pathway in Endotoxin-Induced Acute Lung Injury
    Authors: VY Su, KY Yang, SH Chiou, NJ Chen, MH Mo, CS Lin, CT Wang
    Stem Cells, 2019-02-16;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  10. The Specific Mitogen- and Stress-Activated Protein Kinase MSK1 Inhibitor SB-747651A Modulates Chemokine-Induced Neutrophil Recruitment
    Authors: M Hossain, E Omran, N Xu, L Liu
    Int J Mol Sci, 2017-10-17;18(10):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  11. Malondialdehyde epitopes are sterile mediators of hepatic inflammation in hypercholesterolemic mice
    Authors: Clara Jana-Lui Busch
    Hepatology, 2017-02-03;0(0):.
    Applications: ELISA (Standard)
  12. Main pathways of action of Brazilian red propolis on the modulation of neutrophils migration in the inflammatory process
    Authors: Pedro Luiz Rosalen
    Phytomedicine, 2016-09-28;23(13):1583-1590.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Endothelial LSP1 Modulates Extravascular Neutrophil Chemotaxis by Regulating Nonhematopoietic Vascular PECAM-1 Expression.
    Authors: Hossain M, Qadri S, Xu N, Su Y, Cayabyab F, Heit B, Liu L
    J Immunol, 2015-08-03;195(5):2408-16.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  14. DAP12 expression in lung macrophages mediates ischemia/reperfusion injury by promoting neutrophil extravasation.
    Authors: Spahn J, Li W, Bribriesco A, Liu J, Shen H, Ibricevic A, Pan J, Zinselmeyer B, Brody S, Goldstein D, Krupnick A, Gelman A, Miller M, Kreisel D
    J Immunol, 2015-03-11;194(8):4039-48.
    Species: Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: Bioassay, In Vivo
  15. Mutation in the CD45 inhibitory wedge modulates integrin activation and leukocyte recruitment during inflammation.
    Authors: Germena G, Volmering S, Sohlbach C, Zarbock A
    J Immunol, 2014-12-10;194(2):728-38.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Interference with glycosaminoglycan-chemokine interactions with a probe to alter leukocyte recruitment and inflammation in vivo.
    Authors: Li S, Pettersson U, Hoorelbeke B, Kolaczkowska E, Schelfhout K, Martens E, Kubes P, Van Damme J, Phillipson M, Opdenakker G
    PLoS ONE, 2014-08-05;9(8):e104107.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  17. MyD88-, but not Nod1- and/or Nod2-deficient mice, show increased susceptibility to polymicrobial sepsis due to impaired local inflammatory response.
    Authors: Sonego F, Castanheira F, Czaikoski P, Kanashiro A, Souto F, Franca R, Nascimento D, Freitas A, Spiller F, Cunha L, Zamboni D, Alves-Filho J, Cunha F
    PLoS ONE, 2014-08-01;9(8):e103734.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  18. Macrophage-inducing FasL on chondrocytes forms immune privilege in cartilage tissue engineering, enhancing in vivo regeneration.
    Authors: Fujihara Y, Takato T, Hoshi K
    Stem Cells, 2014-05-01;32(5):1208-19.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  19. Fibrates ameliorate the course of bacterial sepsis by promoting neutrophil recruitment via CXCR2.
    Authors: Tancevski I, Nairz M, Duwensee K, Auer K, Schroll A, Heim C, Feistritzer C, Hoefer J, Gerner R, Moschen A, Heller I, Pallweber P, Li X, Theurl M, Demetz E, Wolf A, Wolf D, Eller P, Ritsch A, Weiss G
    EMBO Mol Med, 2014-04-06;6(6):810-20.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  20. G protein-coupled receptor kinase 6 deficiency promotes angiogenesis, tumor progression, and metastasis.
    Authors: Raghuwanshi S, Smith N, Rivers E, Thomas A, Sutton N, Hu Y, Mukhopadhyay S, Chen X, Leung T, Richardson R
    J Immunol, 2013-04-15;190(10):5329-36.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA (Standard)
  21. Inefficient antimicrobial functions of innate phagocytes render infant mice more susceptible to bacterial infection.
    Authors: Zhang Q, Coveney A, Yu S, Liu J, Li Y, Blankson S, Redmond H, Wang J, Wang J
    Eur J Immunol, 2013-03-22;43(5):1322-32.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  22. alpha-Acid glycoprotein decreases neutrophil migration and increases susceptibility to sepsis in diabetic mice.
    Authors: Spiller F, Carlos D, Souto FO, de Freitas A, Soares FS, Vieira SM, Paula FJ, Alves-Filho JC, Cunha FQ
    Diabetes, 2012-03-13;61(6):1584-91.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  23. IL-17RA signaling amplifies antibody-induced arthritis.
    Authors: Sadik CD, Kim ND, Alekseeva E, Luster AD
    PLoS ONE, 2011-10-20;6(10):e26342.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  24. Diesel exhaust particulates exacerbate asthma-like inflammation by increasing CXC chemokines.
    Authors: Kim J, Natarajan S, Vaickus LJ, Bouchard JC, Beal D, Cruikshank WW, Remick DG
    Am. J. Pathol., 2011-10-01;179(6):2730-9.
    Applications: ELISA (Standard)
  25. Proinflammatory clearance of apoptotic neutrophils induces an IL-12(low)IL-10(high) regulatory phenotype in macrophages.
    Authors: Filardy AA, Pires DR, Nunes MP, Takiya CM, Freire-de-Lima CG, Ribeiro-Gomes FL, DosReis GA
    J. Immunol., 2010-07-21;185(4):2044-50.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  26. A chemotactic gradient sequestered on endothelial heparan sulfate induces directional intraluminal crawling of neutrophils.
    Authors: Massena S, Christoffersson G, Hjertstrom E, Zcharia E, Vlodavsky I, Ausmees N, Rolny C, Li JP, Phillipson M
    Blood, 2010-06-08;116(11):1924-31.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  27. Prostaglandin mediates IL-23/IL-17-induced neutrophil migration in inflammation by inhibiting IL-12 and IFNgamma production.
    Authors: Lemos HP, Grespan R, Vieira SM, Cunha TM, Verri WA, Fernandes KS, Souto FO, McInnes IB, Ferreira SH, Liew FY, Cunha FQ
    Proc. Natl. Acad. Sci. U.S.A., 2009-03-16;106(14):5954-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  28. ST2 deficient mice display a normal host defense against pulmonary infection with Mycobacterium tuberculosis.
    Authors: Wieland CW, van der Windt GJ, Florquin S, McKenzie AN, van der Poll T
    Microbes Infect., 2009-03-13;11(4):524-30.
    Applications: ELISA (Standard)
  29. CD14 contributes to pulmonary inflammation and mortality during murine tuberculosis.
    Authors: Wieland CW, van der Windt GJ, Wiersinga WJ, Florquin S, van der Poll T
    Immunology, 2008-04-03;125(2):272-9.
    Applications: ELISA (Standard)
  30. Bv8 regulates myeloid-cell-dependent tumour angiogenesis.
    Authors: Shojaei F, Wu X, Zhong C, Yu L, Liang XH, Yao J, Blanchard D, Bais C, Peale FV, van Bruggen N, Ho C, Ross J, Tan M, Carano RA, Meng YG, Ferrara N
    Nature, 2007-12-06;450(7171):825-31.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  31. P-selectin glycoprotein ligand-1 regulates chemokine-dependent leukocyte recruitment in colonic ischemia-reperfusion.
    Authors: Santen S, Schramm R, Menger MD, Wang Y, Jeppsson B, Thorlacius H
    Inflamm. Res., 2007-11-01;56(11):452-8.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  32. Mutations in the cofilin partner Aip1/Wdr1 cause autoinflammatory disease and macrothrombocytopenia.
    Authors: Kile BT, Panopoulos AD, Stirzaker RA, Hacking DF, Tahtamouni LH, Willson TA, Mielke LA, Henley KJ, Zhang JG, Wicks IP, Stevenson WS, Nurden P, Watowich SS, Justice MJ
    Blood, 2007-05-21;110(7):2371-80.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  33. Leukocyte PI3Kgamma and PI3Kdelta have temporally distinct roles for leukocyte recruitment in vivo.
    Authors: Liu L, Puri KD, Penninger JM, Kubes P
    Blood, 2007-05-08;110(4):1191-8.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  34. Normal neutrophil functions in sphingosine kinase type 1 and 2 knockout mice.
    Authors: Zemann B, Urtz N, Reuschel R, Mechtcheriakova D, Bornancin F, Badegruber R, Baumruker T, Billich A
    Immunol. Lett., 2007-01-30;109(1):56-63.
    Species: Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: Bioassay, In Vivo
  35. Murine CXCR1 is a functional receptor for GCP-2/CXCL6 and interleukin-8/CXCL8.
    Authors: Fan X, Patera AC, Pong-Kennedy A, Deno G, Gonsiorek W, Manfra DJ, Vassileva G, Zeng M, Jackson C, Sullivan L, Sharif-Rodriguez W, Opdenakker G, Van Damme J, Hedrick JA, Lundell D, Lira SA, Hipkin RW
    J. Biol. Chem., 2006-12-29;282(16):11658-66.
    Species: Mouse
    Sample Types: Complex Sample Type
    Applications: Binding Assay
  36. Overexpression of suppressor of cytokine signaling-5 in T cells augments innate immunity during septic peritonitis.
    Authors: Watanabe H, Kubo M, Numata K, Takagi K, Mizuta H, Okada S, Ito T, Matsukawa A
    J. Immunol., 2006-12-15;177(12):8650-7.
    Applications: ELISA (Standard)
  37. Lack of in vitro and in vivo recognition of Francisella tularensis subspecies lipopolysaccharide by Toll-like receptors.
    Authors: Hajjar AM, Harvey MD, Shaffer SA, Goodlett DR, Sjostedt A, Edebro H, Forsman M, Bystrom M, Pelletier M, Wilson CB, Miller SI, Skerrett SJ, Ernst RK
    Infect. Immun., 2006-09-18;74(12):6730-8.
    Applications: ELISA (Standard)
  38. STAT3 governs distinct pathways in emergency granulopoiesis and mature neutrophils.
    Authors: Panopoulos AD, Zhang L, Snow JW, Jones DM, Smith AM, El Kasmi KC, Liu F, Goldsmith MA, Link DC, Murray PJ, Watowich SS
    Blood, 2006-08-03;108(12):3682-90.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  39. Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
    Authors: Osuchowski MF, Welch K, Siddiqui J, Remick DG
    J. Immunol., 2006-08-01;177(3):1967-74.
    Applications: ELISA (Standard)
  40. Neutrophil recruitment in immunized mice depends on MIP-2 inducing the sequential release of MIP-1alpha, TNF-alpha and LTB(4).
    Authors: Ramos CD, Fernandes KS, Canetti C, Teixeira MM, Silva JS, Cunha FQ
    Eur. J. Immunol., 2006-08-01;36(8):2025-34.
    Applications: ELISA (Standard)
  41. Adenoviral-mediated overexpression of SOCS3 enhances IgG immune complex-induced acute lung injury.
    Authors: Gao H, Hoesel LM, Guo RF, Rancilio NJ, Sarma JV, Ward PA
    J. Immunol., 2006-07-01;177(1):612-20.
    Applications: ELISA (Standard)
  42. The injury response of oligodendrocyte precursor cells is induced by platelets, macrophages and inflammation-associated cytokines.
    Authors: Rhodes KE, Raivich G, Fawcett JW
    Neuroscience, 2006-04-21;140(1):87-100.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  43. High expression of ligands for chemokine receptor CXCR2 in alveolar epithelial neoplasia induced by oncogenic kras.
    Authors: Wislez M, Fujimoto N, Izzo JG, Hanna AE, Cody DD, Langley RR, Tang H, Burdick MD, Sato M, Minna JD, Mao L, Wistuba I, Strieter RM, Kurie JM
    Cancer Res., 2006-04-15;66(8):4198-207.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  44. Human CXCR2 (hCXCR2) takes over functionalities of its murine homolog in hCXCR2 knockin mice.
    Authors: Mihara K, Smit MJ, Krajnc-Franken M, Gossen J, Rooseboom M, Dokter W
    Eur. J. Immunol., 2005-09-01;35(9):2573-82.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  45. Sequential ELISA to profile multiple cytokines from small volumes.
    Authors: Osuchowski MF, Siddiqui J, Copeland S, Remick DG
    J. Immunol. Methods, 2005-07-01;302(1):172-81.
    Applications: ELISA (Standard)
  46. Transcriptional profiling of lipopolysaccharide-induced acute lung injury.
    Authors: Jeyaseelan S, Chu HW, Young SK, Worthen GS
    Infect. Immun., 2004-12-01;72(12):7247-56.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development
  47. The class A macrophage scavenger receptor attenuates CXC chemokine production and the early infiltration of neutrophils in sterile peritonitis.
    Authors: Cotena A, Platt N
    J. Immunol., 2004-11-15;173(10):6427-32.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  48. The role of CCL21 in recruitment of T-precursor cells to fetal thymi.
    Authors: Ueno T, Kuse S, Saito F, Nitta T, Kakiuchi T, Hollander GA, Takahama Y
    Blood, 2004-09-09;105(1):31-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  49. Neutrophil-mediated mycobacteriocidal immunity in the lung during Mycobacterium bovis BCG infection in C57BL/6 mice.
    Authors: Fulton SA, Reba SM, Martin TD, Boom WH
    Infect. Immun., 2002-09-01;70(9):5322-7.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo

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Reviews for Recombinant Mouse CXCL2/MIP-2 Protein

Average Rating: 4.3 (Based on 3 Reviews)

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Recombinant Mouse CXCL2/MIP-2 Protein
By Anonymous on 11/13/2019
Application: Cell migration/motility
Reason for Rating: Worked well in in vitro chemotaxis assay.

Recombinant Mouse CXCL2/MIP-2 Protein
By Jim Hsiao on 04/17/2018
Application: Cell migration/motility

A total of 10^6 mouse neutrophils was added to an insert of a transwell. Recombinant mouse MIP-2 (100 ng/ml) was used as a neutrophil chemoattractant and was added to the lower chamber. After 2 hours of incubation, migrated neutrophils in the lower chambers were enumerated.


Recombinant Mouse CXCL2/MIP-2 Protein
By Anonymous on 01/05/2018
Application: Immunoassay Standard

We made a sandwich ELISA for Mouse MIP-2. MAB452 was used as the capture. BAF452 was used as the detection. 452-M2 was used as the immunoassay standard. It worked well.