Recombinant Mouse DC-SIGN/CD209 Protein, CF

Catalog #: 8345-DC Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
8345-DC-050

R&D Systems Recombinant Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse DC-SIGN/CD209 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE with silver staining

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse DC-SIGN is coated at 1 μg/mL (100 μL/well), the concentration of Recombinant Mouse ICAM-5 Fc Chimera (Catalog # 1173-M5) that produces 50% optimal binding response is 0.8-4 μg/mL.

Source

Mouse myeloma cell line, NS0-derived mouse DC-SIGN/CD209 protein
Val73-Lys238, with an N-terminal HA tag

Accession #

Q91ZX1

N-terminal Sequence
Analysis

Tyr (HA tag)

Predicted Molecular Mass

20 kDa

SDS-PAGE

21-26 kDa, reducing conditions

Product Datasheets

8345-DC

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8345-DC

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: DC-SIGN/CD209

Mouse Dendritic Cell-specific ICAM-3 Grabbing Non-integrin (DC-SIGN)/CD209, also known as CD209 Antigen-like Protein A, is a member of the C-type lectin family (1). Mouse DC-SIGN/CD209 is a 33 kDa, 238 amino acid (aa) type II transmembrane protein (2). The extracellular region contains a Ca²⁺-dependent carbohydrate-binding lectin domain (2). In addition to the full-length mouse DC-SIGN/CD209, a second, truncated splice variant has been reported. DC-SIGN/CD209 is not well conserved between mouse and human with the extracellular domain sharing only 63% aa identity. The DC-SIGN/CD209 lectin domain binds mannose oligosaccharides on pathogens including HIV as well as self glycoproteins including ICAMs (2-4). DC-SIGN/CD209 is expressed on dendritic cells (DC) and inflammatory macrophages and contributes to antigen presentation (2, 5, 6).

References

Liu, W. et al. (2004) J. Biol. Chem. 279:18748.
Caminschi, I. et al. (2001) Mol. Immunol. 38:365.
Curtis, B.M. et al. (1992) Proc. Natl. Acad. Sci. USA 89:8356.
Anthony, R.M. et al. (2008) Proc. Natl. Acad. Sci. USA 105:19571.
Geijtenbeek, T.B. et al. (2000) Cell 100:575.
Garcia-Vallejo, J.J. and Y. van Kooyk (2013) Trends Immunol. 34:482.

Long Name

Dendritic Cell-specific ICAM-3-grabbing Non-integrin 1

Entrez Gene IDs

30835 (Human); 170786 (Mouse); 102121984 (Cynomolgus Monkey)

Alternate Names

CD209 antigendendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbingnon-integrin; CD209 molecule; CD209; CDSIGNHIV gpl20-binding protein; CLEC4L; CLEC4LC-type lectin domain family 4 member L; DCSIGN; DC-SIGN; DC-SIGN1; DC-SIGN1C-type lectin domain family 4, member L; DC-SIGNMGC129965; Dendritic cell-specific ICAM-3-grabbing non-integrin 1