Recombinant Mouse DDR1 Fc Chimera Protein, CF

Catalog #: 6416-DR Datasheet / COA / SDS

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6416-DR-050

R&D Systems Recombinant Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse DDR1 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized Collagen I at 10 µg/mL (100 µL/well) can bind Recombinant Mouse DDR1 Fc Chimera with an apparent K_D < 20 nM.
Optimal dilutions should be determined by each laboratory for each application.

Source

Mouse myeloma cell line, NS0-derived mouse DDR1 protein

Mouse DDR1 (Met1 - Thr414) Accession # Q03146	IEGRMDP	Mouse IgG_2A (Glu98 - Lys330)
N-terminus		C-terminus

Accession #

Q03146

N-terminal Sequence
Analysis

Asp22

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

71.1 kDa (monomer)

SDS-PAGE

85-95 kDa, reducing conditions

Product Datasheets

6416-DR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6416-DR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 300 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: DDR1

DDR1, also known as CAK, NEP, CD167a, and EDDR1, is a 120 - 140 kDa type I transmembrane glycoprotein belonging to the discoidin-like domain‑containing subfamily of receptor tyrosine kinases (1, 2). Mature mouse DDR1 consists of a 395 aa extracellular domain (ECD) that includes the discoidin-like domain, a 27 aa transmembrane segment, and a 470 aa cytoplasmic domain with the tyrosine kinase domain (3). Within the ECD, mouse DDR1 shares 93% aa sequence identity with human and rat DDR1, respectively. Alternate splicing of mouse DDR1 generates an additional isoform that lacks a portion of the cytoplasmic domain encompassing the Shc-interacting NPxY motif (4). DDR1 is expressed on epithelial tissues, activated monocytes and neutrophils, smooth muscle cells, and in several cancers (1, 2). It mediates cellular adhesion and migration through interaction of the discoidin-like domain with the triple helical structure of collagens I ‑ V (5, 6). Collagen binding induces prolonged tyrosine autophosphorylation of DDR1, including within the NPxY motif (5, 6). Collagen binding can also induce the metalloproteinase-dependent cleavage of DDR1, thereby liberating a tyrosine phosphorylated 60 kDa C-terminal fragment and a 60 kDa ECD fragment (7, 8). DDR1 is expressed as a dimer on the cell surface independently of ligand binding (9). Oligomerization enhances collagen binding and also modulates collagen fibrillogenesis (10, 11). Collagen-induced activation of DDR1 is inhibited by the association of DDR1 with E-Cadherin at epithelial cell junctions (12). Expression of DDR1 on arterial smooth muscle cells limits smooth muscle cell migration and the development of proteoglycan plaques during atherogenesis (13). The overexpression of particular DDR1 isoforms in glioblastoma promotes MMP-2 activation and increased tumor cell invasiveness (14).

References

Vogel, W.F. et al. (2006) Cell. Signal. 18:1108.
Yoshimura, T. et al. (2005) Immunol. Res. 31:219.
Zerlin, M. et al. (1993) Oncogene 8:2731.
Perez, J.L. et al. (1996) Oncogene 12:1469.
Shrivastava, A. et al. (1997) Mol. Cell 1:25.
Vogel, W. et al. (1997) Mol. Cell 1:13.
Slack, B.E. et al. (2006) J. Cell Biochem. 98:672.
Vogel, W.F. et al. (2001) FEBS Lett. 514:175.
Noordeen, N.A. et al. (2006) J. Biol. Chem. 281:22744.
Leitinger, B. (2003) J. Biol. Chem. 278:16761.
Agarwal, G. et al. (2007) J. Mol. Biol. 367:443.
Wang, C.Z et al. (2009) Am. J. Physiol. Cell Physiol. 297:C419.
Franco, C. et al. (2010) Circ. Res. 106:1775.
Ram, R. et al. (2006) J. Neurooncol. 76:239.

Long Name

Discoidin Domain Receptor 1

Entrez Gene IDs

780 (Human)

Alternate Names

CAK; CD167 antigen-like family member A; CD167a antigen; CD167a; DDR; DDR1; discoidin domain receptor family, member 1; discoidin domain receptor tyrosine kinase 1; Discoidin receptor tyrosine kinase; EC 2.7.10; EC 2.7.10.1; EDDR1; ENTRK4; Epithelial discoidin domain receptor 1; HGK2; MCK10; MCK-10; NTRK4; Protein-tyrosine kinase 3A; PTK3A protein tyrosine kinase 3A; PTK3A; receptor, type 4; RTK6; TRK E; TrkE