Recombinant Mouse DDR2 Fc Chimera Protein, CF

Catalog #: 7479-DR Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
7479-DR-050

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse DDR2 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized Collagen I at 10 µg/mL (100 µL/well) can bind Recombinant Mouse DDR2 Fc Chimera with an apparent K_D <10 nM.
Optimal dilutions should be determined by each laboratory for each application.

Source

Mouse myeloma cell line, NS0-derived mouse DDR2 protein

Mouse DDR2 (Gln24-Arg399) Accession # NP_072075	IEGRMDP	Mouse IgG_2A (Glu98-Lys330)
N-terminus		C-terminus

Accession #

NP_072075

N-terminal Sequence
Analysis

Gln24 predicted: No results obtained, sequencing might be blocked

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

69.6 kDa (monomer)

SDS-PAGE

80-90 kDa, reducing conditions

Product Datasheets

7479-DR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7479-DR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: DDR2

DDR2, also known as TYR010 and TKT, is a widely expressed 130 kDa type I transmembrane glycoprotein belonging to the discoidin‑like domain containing subfamily of receptor tyrosine kinases (1). Mature mouse DDR2 consists of a 378 amino acid (aa) extracellular domain (ECD) that includes the discoidin-like domain, a 22 aa transmembrane segment, and a 433 aa cytoplasmic domain that includes the kinase domain (2). Within the ECD, mouse DDR2 shares 50% aa sequence identity with DDR1. It shares 97% and 99% aa sequence identity with human and rat DDR2, respectively. The discoidin-like domain mediates DDR2 interactions with collagens I, III, and X (3‑5). Collagens II and V are less efficacious ligands (3). DDR2 selectively recognizes the triple helical structure of collagen compared to monomeric or denatured collagen (3, 5, 6). Within collagen II, the D2 period is required for DDR2 binding. The D1 period is additionally required to trigger DDR2 autophosphorylation (6). The ECD of DDR2 exists as a noncovalent dimer in solution, and dimerization of the receptor greatly enhances collagen binding (4, 7). DDR2 interaction with collagen I inhibits collagen fibrillogenesis and alters collagen fiber morphology (7). Ligand binding induces DDR2 autophosphorylation in the cytoplasmic domain (3, 5, 8), which promotes associations with Shc and Src (9). In addition to the above mechanism, DDR2 exhibits a distinct interaction with collagen X. A region other than the discoidin-like domain of DDR2 recognizes the non-helical NC1 domain of collagen X, and this interaction does not lead to receptor autophosphorylation (5). Activation of DDR2 by collagen induces up‑regulation of MMP-1, -2, and -13 as well as DDR2 itself (3, 8, 10). DDR2 is implicated in collagenous matrix destruction and cell invasiveness (8, 10). It promotes osteoblast and chondrocyte differentiation and supports ovulation and spermatogenesis (11‑13). DDR2 is up‑regulated in several pathological conditions, including hepatic fibrosis following injury, rheumatoid and osteoarthritis, and smooth muscle cell overgrowth diseases (8, 10, 14, 15).

References

Vogel, W.F. et al. (2006) Cell. Signal. 18:1108.
Karn, T. et al. (1993) Oncogene 8:3433.
Vogel, W. et al. (1997) Mol. Cell 1:13.
Leitinger, B. (2003) J. Biol. Chem. 278:16761.
Leitinger, B. and Kwan, A.P.L. (2006) Matrix Biol. 25:355.
Leitinger, B. et al. (2004) J. Mol. Biol. 344:993.
Mihai, C. et al. (2006) J. Mol. Biol. 361:864.
Olaso, E. et al. (2001) J. Clin. Invest. 108:1369.
Ikeda, K. et al. (2002) J. Biol. Chem. 277:19206.
Xu, L. et al. (2005) J. Biol. Chem. 280:548.
Zhang, Y. et al. (2011) J. Bone Miner. Res. 26:604.
Matsumura, H. et al. (2009) Physiol. Genomics 39:120.
Kano, K. et al. (2010) Mol. Reprod. Dev. 77:29.
Wang, J. et al. (2002) J. Autoimmun. 19:161.
Ferri, N. et al. (2004) Am. J. Pathol. 164:1575.

Long Name

Discoidin Domain Receptor 2

Entrez Gene IDs

4921 (Human); 18214 (Mouse)

Alternate Names

CD167 antigen-like family member B; CD167b antigen; DDR2; Discoidin domain receptor 2; discoidin domain receptor family, member 2; discoidin domain receptor tyrosine kinase 2; discoidin domain-containing receptor 2; EC 2.7.10; EC 2.7.10.1; hydroxyaryl-protein kinase; migration-inducing gene 16 protein; neurotrophic tyrosine kinase receptor related 3; Neurotrophic tyrosine kinase, receptor-related 3; NTRKR3cell migration-inducing protein 20; Receptor protein-tyrosine kinase TKT; TKT; TKTMIG20a; Trk3; TYRO10; Tyro-10; Tyrosine-protein kinase TYRO10; tyrosylprotein kinase