Recombinant Mouse DR6/TNFRSF21 Fc Chimera Protein, CF

Catalog #: 6985-DR Datasheet / COA / SDS

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6985-DR-050

R&D Systems Recombinant Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse DR6/TNFRSF21 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA.

When recombinant human APP770 is coated at 2 μg/mL (100 μL/well), the concentration of Recombinant Mouse DR6/TNFRSF21 Fc Chimera that produces 50% of the optimal binding response is found to be approximately 80-400 ng/mL.

Source

Mouse myeloma cell line, NS0-derived mouse DR6/TNFRSF21 protein

Mouse DR6 (Met1 - His349) Accession # NP_848704	IEGRMDP	Mouse IgG_2A(Glu98 - Lys330)
N-terminus		C-terminus

Accession #

NP_848704

N-terminal Sequence
Analysis

No results obtained: Gln42 predicted, N-sequencing might be blocked

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

60.7 kDa (monomer)

SDS-PAGE

80-95 kDa, reducing conditions

Product Datasheets

6985-DR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6985-DR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: DR6/TNFRSF21

Death Receptor 6 (DR6), also known as TNFRSF21 and CD358, is a type I transmembrane protein in the TNF receptor superfamily (1). Mouse DR6 consists of a 308 amino acid (aa) extracellular domain (ECD) with four cysteine-rich motifs, a 21 aa transmembrane segment, and a 285 aa palmityolated cytoplasmic region that contains one death domain (2, 3). Within the ECD, mouse and human DR6 share 82% aa sequence identity. DR6 is expressed as an approximately 110 kDa molecule that carries extensive N-linked and O-linked glycosylation in its extracellular region (3, 4). Among hematopoietic cells, DR6 is expressed on monocytes, resting CD4+ T cells, and pro-, pre-, and naïve B cells (5 - 7). DR6 knockout mice exhibit a Th2-biased immune response characterized by exaggerated Th2 and B cell responsiveness in combination with reduced Th1 cell responsiveness and inflammatory leukocyte infiltration (2, 6 - 8). DR6 knockout mice are resistant to induced airway inflammation and experimental autoimmune encephalitis but more susceptible to severe graft versus host disease (8 - 10). DR6 is also expressed on developing neurons where it can bind a shed 35 kDa N-terminal fragment of APP or a fragment of APLP2 (11, 12). This APP fragment is generated following deprivation of neurotrophic factors, and its binding to DR6 triggers DR6-mediated axonal pruning (11). DR6 is constitutively expressed on some prostate cancer cells and can be induced by TNF-alpha on others (3, 4).

References

Benschop, R. et al. (2009) Adv. Exp. Med. Biol. 647:186.
Zhao, H. et al. (2001) J. Exp. Med. 194:1441.
Klima, M. et al. (2009) Biochim. Biophys. Acta 1793:1579.
Kasof, G.M. et al. (2001) Oncogene 20:7965.
Matesanz-Isabel, J. et al. (2011) Immunol. Lett. 134:104.
Schmidt, C.S. et al. (2003) J. Exp. Med. 197:51.
Liu, J. et al. (2001) Immunity 15:23.
Venkataraman, C. et al. (2006) Immunol. Lett. 106:42.
Schmidt, C.S. et al. (2005) J. Immunol. 175:2286.
Liu, J. et al. (2002) J. Immunol. 169:3993.
Nikolaev, A. et al. (2009) Nature 457:981.
Kuester, M. et al. (2011) J. Mol. Biol. 409:189.

Long Name

Death Receptor 6

Entrez Gene IDs

27242 (Human); 94185 (Mouse); 316256 (Rat)

Alternate Names

BM-018; CD358 antigen; CD358; Death receptor 6; DR6; DR6TNFR-related death receptor 6; MGC31965; TNFRSF21; tumor necrosis factor receptor superfamily member 21; tumor necrosis factor receptor superfamily, member 21