Recombinant Mouse EG-VEGF/PK1 Protein

Catalog #: 2464-EV Datasheet / COA / SDS

Carrier Free

Catalog #	Availability	Size / Price	Qty
2464-EV-025/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
2464-EV-025

R&D Systems Recombinant Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse EG-VEGF/PK1 Protein Summary

Product Specifications

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured in a cell proliferation assay using human pancreatic cancer cells. The ED₅₀ for this effect is 1-4 μg/mL. Measured in a cell proliferation assay using EJG bovine adrenal-derived endothelial cells. The ED₅₀ for this effect is 1-4 µg/mL.

Source

Mouse myeloma cell line, NS0-derived mouse EG-VEGF/PK1 protein
Ala20-Phe105

Accession #

Q14A28

N-terminal Sequence
Analysis

Ala20

Predicted Molecular Mass

9.6 kDa

SDS-PAGE

9.6 kDa, reducing conditions

Product Datasheets

2464-EV (with carrier)

Product Datasheet

COA

SDS

2464-EV/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2464-EV

Formulation	Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after opening. 3 months, -20 to -70 °C under sterile conditions after opening.

2464-EV/CF

Formulation	Supplied as a 0.2 μm filtered solution in PBS.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after opening. 3 months, -20 to -70 °C under sterile conditions after opening.

Reconstitution Calculator

Background: EG-VEGF/PK1

Endocrine gland-derived vascular endothelial cell growth factor (EG-VEGF; also called prokineticin-1) is a member of the prokineticin family of secreted proteins whose distinguishing structural motif involves 10 cysteines that form five intrachain disulfide bonds, creating a “colipase fold” (1, 2). The mouse EG‑VEGF precursor is 105 amino acids in length and contains a 19 aa signal sequence plus an 86 aa mature segment (3). There are no potential N‑linked glycosylation sites, and the secreted molecule is a monomer that is approximately 10 kDa in size. The mature region in mouse is 93% and 87% aa identical to the mature regions in rat and human, respectively. Mature mouse prokineticin-2 is 57% aa identical to mouse EG‑VEGF/prokineticin-1. Mouse EG‑VEGF is active on bovine endothelial cells (3). Although there is considerable aa identity between mouse and human EG-VEGF, the expression patterns are very much different. This is attributed to pronounced differences in the promoter region of the two genes. While human EG-VEGF is expressed by steroidogenic glands, mouse EG‑VEGF is expressed in hepatocytes and renal tubular epithelial cells. EG-VEGF binds to and activates two closely related G-protein coupled receptors, EG‑VEGF/PK1‑R1 and EG‑VEGF/PK2‑R2 (4, 5). Mouse EG‑VEGF stimulates proliferation and survival of liver sinusoidal endothelial cells, and, in mouse, EG-VEGF acts to induce monocyte migration and stimulate hematopoiesis (3, 6).

References

LeCouter, J. et al. (2004) Ann. N.Y. Acad. Sci. 1014:50.
Li, M. et al. (2001) Mol. Pharmacol. 59:692.
LeCouter, J. et al. (2003) Endocrinology 144:2606.
Lin, D. et al. (2002) J. Biol. Chem. 277:19276.
Masuda, Y. et al. (2002) Biochem. Biophys. Res. Commun. 293:396.
LeCouter, J. et al. (2004) Proc. Natl. Acad. Sci. USA 101:16813.

Long Name

Endocrine Gland-derived Vascular Endothelial Growth Factor

Entrez Gene IDs

84432 (Human); 246691 (Mouse); 192205 (Rat)

Alternate Names

EGVEGF; EG-VEGF; EGVEGFblack mamba toxin-related protein; Endocrine-gland-derived vascular endothelial growth factor; Mambakine; PK1mambakine; PRK1prokineticin-1; PROK1; Prokineticin 1