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Recombinant Mouse EphB3 Fc Chimera Protein, CF

Catalog #: 432-B3
4 Citations Datasheet / COA / SDS

Discontinued Product

432-B3 has been discontinued.
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Recombinant Mouse EphB3 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse EphB3 Fc Chimera at 2 µg/mL (100 µL/well) can bind recombinant mouse Ephrin-B1 Fc Chimera with a linear range of 0.078-1.25 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse EphB3 protein
Mouse EphB3
(Leu30-Thr537)
Accession # P54754		 DIEGRMD Human IgG1

(Pro100-Lys330)

 HHHHHH
N-terminus C-terminus
Accession #
P54754
N-terminal Sequence
Analysis
Leu30
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
82.8 kDa (monomer)
SDS-PAGE
100 kDa, reducing conditions

Product Datasheets

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432-B3

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

432-B3

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: EphB3

EphB3, also known as Cek10, Tyro6, Sek4, Hek2, and Mdk5, is a 130 kDa member of the transmembrane Eph receptor tyrosine kinase family. The A and B classes of Eph proteins are distinguished by Ephrin ligand binding preference but have a common structural organization. Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression (1). The 525 amino acid (aa) extracellular domain (ECD) of mature mouse EphB3 contains a ligand binding domain followed by a cysteine rich region and two fibronectin type III domains. The 418 aa cytoplasmic domain contains a tyrosine kinase domain, a sterile alpha motif (SAM), and a PDZ binding motif (2). Within the ECD, mouse EphB3 shares 96% and 99% aa sequence identity with human and rat EphB3, respectively. Binding of EphB3 to its ligands Ephrin-B1, B2, and B3 triggers forward signaling through EphB3 as well as reverse signaling through the Ephrin (1, 3). EphB3 also interacts in cis with the receptor tyrosine kinase Ryk (4). Activation of its kinase is required for some but not all of the effects of EphB3 on cellular adhesion, motility, and morphology (5). EphB3 is widely expressed during development and in the adult; it shows a complementary tissue distribution to the Ephrin-B ligands (6-9). EphB3 function is important in vascular, nervous system, thymocyte, and palate development (6, 7, 10-12). It directs embyronic neuronal axon pathfinding, and its up-regulation on local macrophages following neuronal injury promotes the growth of regenerating axons (10, 13). EphB3 inhibits colorectal carcinogenesis and invasion by preventing the migration of tumor cells out of the intestinal crypt (9, 14). In non-small cell lung cancer, however, it is up-regulated and can promote tumor progression (15). EphB3 function is supported by the cooperative action of EphB2 in several of these processes (6, 10-12, 16).

References
  1. Park, I. and H.S. Lee (2015) Mol. Cells 38:14.
  2. Ruiz, J.C. et al. (1994) Mech. Dev. 48:153.
  3. Pasquale, E.B (2004) Nat. Neurosci. 7:417.
  4. Trivier, E. and T.S. Ganesan (2002) J. Biol. Chem. 277:23037.
  5. Miao, H. et al. (2005) J. Biol. Chem. 280:923.
  6. Adams, R.H. et al. (1999) Genes Dev. 13:295.
  7. Krull, C.E. et al. (1997) Curr. Biol. 7:571.
  8. Willson, C.A. et al. (2006) J. Mol. Histol. 37:369.
  9. Cortina, C. et al. (2007) Nature Genet. 39:1376.
  10. Birgbauer, E. et al. (2000) Development 127:1231.
  11. Alfaro, D. et al. (2008) Immunology 125:131.
  12. Risley, M. et al. (2009) Mech. Dev. 126:230.
  13. Liu, X. et al. (2006) J. Neurosci. 26:3087.
  14. Batlle, E. et al. (2005) Nature 435:1126.
  15. Ji, X.-D. et al. (2011) Cancer Res. 71:1156.
  16. Holmberg, J. et al. (2006) Cell 125:1151.
Long Name
Eph Receptor B3
Entrez Gene IDs
2049 (Human); 13845 (Mouse)
Alternate Names
Cek10; EC 2.7.10; EC 2.7.10.1; EK2; EPH receptor B3; EphB3; EPH-like kinase 2; EPH-like tyrosine kinase-2; ephrin type-B receptor 3; ETK2Hek2; Hek2; human embryo kinase 2; Mdk5; Sek4; Tyro6; Tyrosine-protein kinase TYRO6

Citations for Recombinant Mouse EphB3 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Ephrin?b3 modulates hippocampal neurogenesis and the reelin signaling pathway in a pilocarpine?induced model of epilepsy
    Authors: TT Liu, Y Li, Y Shu, B Xiao, L Feng
    Int. J. Mol. Med., 2018-03-07;0(0):.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  2. EphB4 forward signalling regulates lymphatic valve development.
    Authors: Zhang, Gu, Brady, John, Liang, Wei-Chin, Wu, Yan, Henkemeyer, Mark, Yan, Minhong
    Nat Commun, 2015-04-13;6(0):6625.
  3. Single amino acid changes in the Nipah and Hendra virus attachment glycoproteins distinguish ephrinB2 from ephrinB3 usage.
    Authors: Negrete OA, Chu D, Aguilar HC, Lee B
    J. Virol., 2007-07-25;81(19):10804-14.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Antiangiogenic and antitumor efficacy of EphA2 receptor antagonist.
    Authors: Dobrzanski P, Hunter K, Jones-Bolin S, Chang H, Robinson C, Pritchard S, Zhao H, Ruggeri B
    Cancer Res., 2004-02-01;64(3):910-9.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay

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