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Recombinant Mouse Ephrin-A2 Fc Chimera Protein, CF

Catalog #: 603-A2
7 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
603-A2-200
R&D Systems Recombinant Proteins and Enzymes
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Citations (7)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Ephrin-A2 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized recombinant rat EphA5 Fc Chimera at 2 µg/mL (100 µL/well) can bind rmEphrin-A2/Fc Chimera with a linear range of 0.16‑10 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Ephrin-A2 protein
Mouse Ephrin-A2
(Glu23-Asn184)
Accession # P52801		 IEGRMD Human IgG1
(Pro100-Lys330)		 6-His tag
N-terminus C-terminus
Accession #
P52801
N-terminal Sequence
Analysis
Glu23
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
46 kDa (monomer)
SDS-PAGE
57 kDa, reducing conditions

Product Datasheets

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603-A2

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

603-A2

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ephrin-A2

Ephrin-A2, also known as ELF-1, HEK7-L, LERK-6, and EPLG6, is an approximately 20 kDa member of the Ephrin-A family of GPI-anchored ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. Ephrin-A ligands are structurally related to the extracellular domains of the transmembrane Ephrin-B ligands. Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression. Ephrin-A2 preferentially interacts with receptors in the EphA family (1, 2). Mouse Ephrin-A2 is synthesized with a 20 amino acid (aa) signal peptide, a 164 aa mature chain, and a 25 aa C-terminal propeptide which is removed prior to GPI linkage of Ephrin-A2 to the membrane (3, 4). It shares 93% and 100% aa sequence identity with human and rat Ephrin-A2, respectively. Ephrin-A2 is expressed in discrete regions of the developing nervous system and limb buds (4 - 7). Its distribution complements the pattern of Eph receptor expression, and this plays an important role in tissue morphogenesis (7‑9). Ephrin-A2 exerts an axon repulsive signal which is important for the accurate pathfinding of retinal ganglion cell axons to the tectum and hippocampal axons to the lateral septum (8, 10). Its up‑regulation in astrocytes at sites of optic nerve damage may prevent re-innervation by retinal ganglion cells (11). Ephrin-A2 is also expressed on neural progenitor cells in the subventricular zone (SVZ). It interacts with EphA7, triggering reverse signaling through Ephrin-A2 and inhibition of progenitor cell proliferation (9). In the developing limbs, Ephrin-A2 regulates cartilage morphogenesis and the projection of motoneuron axons (6, 7, 12).

References
  1. Miao, H. and B. Wang (2009) Int. J. Biochem. Cell Biol. 41:762.
  2. Pasquale, E.B. (2010) Nat. Rev. Cancer 10:165.
  3. Shao, H. et al. (1995) J. Biol. Chem. 270:3467.
  4. Cheng, H.-J. et al. (1994) Cell 79:157.
  5. Kenmuir, C.L. et al. (2012) Anat. Rec. (Hoboken) 295:105.
  6. Ohta, K. et al. (1997) Mech. Dev. 64:127.
  7. Wada, N. et al. (2003) Dev. Biol. 264:550.
  8. Gao, P.-P. et al. (1996) Proc. Natl. Acad. Sci. USA 93:11161.
  9. Holmberg, J. et al. (2005) Genes Dev. 19:462.
  10. Nakamoto, M. et al. (1996) Cell 86:755.
  11. Symonds, A.C.E. et al. (2007) Eur. J. Neurosci. 25:744.
  12. Eberhart, J. et al. (2000) Dev. Neurosci. 22:237.
Entrez Gene IDs
1943 (Human); 13637 (Mouse)
Alternate Names
Cek7-L; EFNA2; ELF-1; EPH-related receptor tyrosine kinase ligand 6; EphrinA2; Ephrin-A2; EPLG6HEK7 ligand; HEK7-L; HEK7-ligand; LERK6; LERK6LERK-6; ligand of eph-related kinase 6

Citations for Recombinant Mouse Ephrin-A2 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Structural and functional analyses reveal promiscuous and species specific use of ephrin receptors by Cedar virus
    Authors: ED Laing, CK Navaratnar, S Cheliout D, SR Petzing, Y Xu, SL Sterling, GA Marsh, LF Wang, M Amaya, DB Nikolov, R Cattaneo, CC Broder, K Xu
    Proc. Natl. Acad. Sci. U.S.A., 2019-09-23;0(0):.
    Species: Virus - Henipavirus
    Sample Types: Recombinant Protein
    Applications: Coprecipitation Assay
  2. Syntaxin 1B suppresses macropinocytosis and semaphorin 3A-induced growth cone collapse.
    Authors: Kabayama H, Takeuchi M, Taniguchi M
    J. Neurosci., 2011-05-18;31(20):7357-64.
    Species: Chicken
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Cooperative signaling between Slit2 and Ephrin-A1 regulates a balance between angiogenesis and angiostasis.
    Authors: Dunaway CM, Hwang Y, Lindsley CW
    Mol. Cell. Biol., 2010-12-06;31(3):404-16.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  4. Ephrins negatively regulate cell proliferation in the epidermis and hair follicle.
    Authors: Genander M, Holmberg J, Frisen J
    Stem Cells, 2010-07-01;28(7):1196-205.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  5. Neurite responses to ephrin-A5 modulated by BDNF: evidence for TrkB-EphA interactions.
    Authors: Fitzgerald M, Buckley A, Lukehurst SS, Dunlop SA, Beazley LD, Rodger J
    Biochem. Biophys. Res. Commun., 2008-07-30;374(4):625-30.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance
  6. Disruption of Esrom and Ryk identifies the roof plate boundary as an intermediate target for commissure formation.
    Authors: Hendricks M, Mathuru AS, Wang H, Silander O, Kee MZ, Jesuthasan S
    Mol. Cell. Neurosci., 2007-10-16;37(2):271-83.
    Species: Zebrafish
    Sample Types: Whole Tissue
    Applications: IHC
  7. Ephrin stimulation modulates T cell chemotaxis.
    Authors: Sharfe N, Freywald A, Toro A, Dadi H, Roifman C
    Eur. J. Immunol., 2002-12-01;32(12):3745-55.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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