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Recombinant Mouse Ephrin-B1 Fc Chimera Protein, CF

Catalog #: 473-EB
26 Citations 1 Review Datasheet / COA / SDS
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473-EB-200
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Citations (26)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Ephrin-B1 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse EphB3 Fc Chimera at 2 µg/mL (100 µL/well) can bind Recombinant Mouse Ephrin-B1 Fc Chimera with a linear range of 0.01-0.5 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Ephrin-B1 protein
Mouse Ephrin-B1
(Lys30-Ser229)
Accession # AAA53231		 IEGRMD Human IgG1
(Pro100-Lys330)		 6-His tag
N-terminus C-terminus
Accession #
AAA53231
N-terminal Sequence
Analysis
Lys30
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
49.2 kDa (monomer)
SDS-PAGE
60 kDa, reducing conditions

Product Datasheets

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473-EB

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

473-EB

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ephrin-B1

Ephrin-B1, also known as Elk Ligand, LERK2, and Eplg2, is an approximately 45 kDa member of the Ephrin-B family of transmembrane ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. The extracellular domains (ECD) of Ephrin-B ligands are structurally related to GPI-anchored Ephrin-A ligands. Eph‑Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression. Ephrin-B1 preferentially interacts with receptors in the EphB family. The binding of Ephrin-B1 to EphB proteins also triggers reverse signaling through Ephrin-B1 (1, 2). Mature mouse Ephrin-B1 consists of a 212 amino acid (aa) ECD, a 21 aa transmembrane segment, and an 88 aa cytoplasmic domain (3, 4). Within the ECD, mouse Ephrin-B1 shares 94% and 98% aa sequence identity with human and rat Ephrin-B1, respectively. Ligation by EphB2 enhances shedding of a 35 kDa fragment of the Ephrin-B1 ECD (5). The residual membrane-bound portion is then cleaved by gamma-secretase to release the intracellular domain (6). Ephrin-B1 also associates in cis with Claudin-1, -4, and -5 (7, 8). It is expressed on glomerular podocyte slit diaphragms, developing thymocytes, peripheral T cells, monocytes, macrophages, vascular endothelial cells, cardiomyocytes, osteoclasts, and luteinizing granulosa cells in the ovary (8-13). In the developing nervous system, Ephrin-B1 plays a role in cellular migration, axon guidance, and presynaptic development (14-16). It also regulates developing thymocyte survival, monocyte migration, osteoclast differentiation and function, cardiac muscle morphogenesis, and tumorigenesis (5, 8, 10-12). Ephrin-B1 is up-regulated on reactive astrocytes and on macrophages and T cells found in atherosclerotic plaques (11, 17).

References
  1. Miao, H. and B. Wang (2009) Int. J. Biochem. Cell Biol. 41:762.
  2. Pasquale, E.B. (2010) Nat. Rev. Cancer 10:165.
  3. Shao, H. et al. (1994) J. Biol. Chem. 269:26606.
  4. Fletcher, F.A. et al. (1994) Genomics 24:127.
  5. Tanaka, M. et al. (2007) J. Cell Sci. 120:2179.
  6. Tomita, T. et al. (2006) Mol. Neurodegen. 1:2.
  7. Tanaka, M. et al. (2005) EMBO J. 24:3700.
  8. Genet, G. et al. (2012) Circ. Res. 110:688.
  9. Hashimoto, T. et al. (2007) Kidney Int. 72:954.
  10. Yu, G. et al. (2006) J. Biol. Chem. 281:10222.
  11. Sakamoto, A. et al. (2008) Clin. Sci. 114:643.
  12. Cheng, S. et al. (2012) PLoS ONE 7:e32887.
  13. Egawa, M. et al. (2003) J. Clin. Endocrinol. Metab. 88:4384.
  14. Davy, A. et al. (2004) Genes Dev. 18:572.
  15. Bush, J.O. and P. Soriano (2009) Genes Dev. 23:1586.
  16. McClelland, A.C. et al. (2009) Proc. Natl. Acad. Sci. USA 106:20487.
  17. Wang, Y. et al. (2005) Eur. J. Neurosci. 21:2336.
Entrez Gene IDs
1947 (Human); 13641 (Mouse); 25186 (Rat)
Alternate Names
Cek5-L; EFL3; EFL-3; EFNB1; ELK ligand; ELK-L; EphrinB1; Ephrin-B1; LERK-2; STRA-1

Citations for Recombinant Mouse Ephrin-B1 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

26 Citations: Showing 1 - 10
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  1. Ubiquitin ligase and signalling hub MYCBP2 is required for efficient EPHB2 tyrosine kinase receptor function
    Authors: Chang, C;Banerjee, SL;Park, SS;Zhang, X;Cotnoir-White, D;Desbois, M;Grill, B;Kania, A;
    bioRxiv : the preprint server for biology
    Species: Human, Mouse
    Sample Types: Transfected Whole Cells
    Applications: Bioassay
  2. Eph/Ephrin Promotes the Adhesion of Liver Tissue-Resident Macrophages to a Mimicked Surface of Liver Sinusoidal Endothelial Cells
    Authors: S Kohara, K Ogawa
    Biomedicines, 2022-12-12;10(12):.
    Species: Mouse
    Sample Types: Tissue Lysate
    Applications: Bioassay
  3. Structural and functional analyses reveal promiscuous and species specific use of ephrin receptors by Cedar virus
    Authors: ED Laing, CK Navaratnar, S Cheliout D, SR Petzing, Y Xu, SL Sterling, GA Marsh, LF Wang, M Amaya, DB Nikolov, R Cattaneo, CC Broder, K Xu
    Proc. Natl. Acad. Sci. U.S.A., 2019-09-23;0(0):.
    Species: Virus - Henipavirus
    Sample Types: Recombinant Protein
    Applications: Coprecipitation Assay
  4. Cross Talk between One-Carbon Metabolism, Eph Signaling, and Histone Methylation Promotes Neural Stem Cell Differentiation
    Authors: MA Fawal, T Jungas, A Kischel, C Audouard, JS Iacovoni, A Davy
    Cell Rep, 2018-06-05;23(10):2864-2873.e7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Unidirectional Eph/ephrin signaling creates a cortical actomyosin differential to drive cell segregation
    Authors: AK O'Neill, AA Kindberg, TK Niethamer, AR Larson, HH Ho, ME Greenberg, JO Bush
    J. Cell Biol., 2016-10-17;215(2):217-229.
    Applications: Control
  6. Expression of the Receptor Tyrosine Kinase EphB2 on Dendritic Cells Is Modulated by Toll-Like Receptor Ligation but Is Not Required for T Cell Activation.
    Authors: Mimche P, Brady L, Keeton S, Fenne D, King T, Quicke K, Hudson L, Lamb T
    PLoS ONE, 2015-09-25;10(9):e0138835.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. EphB4 forward signalling regulates lymphatic valve development.
    Authors: Zhang, Gu, Brady, John, Liang, Wei-Chin, Wu, Yan, Henkemeyer, Mark, Yan, Minhong
    Nat Commun, 2015-04-13;6(0):6625.
    Species: Mouse
    Sample Types: Antibody
    Applications: Enzyme Assay
  8. A chemical genetic approach reveals distinct EphB signaling mechanisms during brain development.
    Authors: Soskis, Michael, Ho, Hsin-Yi, Bloodgood, Brenda L, Robichaux, Michael, Malik, Athar N, Ataman, Bulent, Rubin, Alex A, Zieg, Janine, Zhang, Chao, Shokat, Kevan M, Sharma, Nikhil, Cowan, Christop, Greenberg, Michael
    Nat Neurosci, 2012-11-11;15(12):1645-54.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  9. PI3K contributed to modulation of spinal nociceptive information related to ephrinBs/EphBs.
    Authors: Yu L, Zhou X, Yu J, Huang H, Jiang L, Zhang F, Cao J, Yan M
    PLoS ONE, 2012-08-03;7(8):e40930.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  10. A novel feedback mechanism by Ephrin-B1/B2 in T-cell activation involves a concentration-dependent switch from costimulation to inhibition.
    Authors: Kawano H, Katayama Y, Minagawa K, Shimoyama M, Henkemeyer M, Matsui T
    Eur. J. Immunol., 2012-05-23;42(6):1562-72.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  11. EphB regulates L1 phosphorylation during retinocollicular mapping.
    Mol. Cell. Neurosci., 2012-05-08;50(2):201-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. EphB receptors trigger Akt activation and suppress Fas receptor-induced apoptosis in malignant T lymphocytes.
    Authors: Maddigan A, Truitt L, Arsenault R
    J. Immunol., 2011-10-28;187(11):5983-94.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. nev (cyfip2) is required for retinal lamination and axon guidance in the zebrafish retinotectal system.
    Authors: Pittman AJ, Gaynes JA, Chien CB
    Dev. Biol., 2010-06-09;344(2):784-94.
    Species: Zebrafish
    Sample Types: Whole Tissue
    Applications: IHC
  14. Astrocyte-produced ephrins inhibit schwann cell migration via VAV2 signaling.
    Authors: Afshari FT, Kwok JC, Fawcett JW
    J. Neurosci., 2010-03-24;30(12):4246-55.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Divergent roles for Eph and ephrin in avian cranial neural crest.
    Authors: Mellott DO, Burke RD
    BMC Dev. Biol., 2008-05-21;8(0):56.
    Species: Chicken
    Sample Types: Whole Tissue
    Applications: IHC
  16. EphB receptors co-distribute with a nicotinic receptor subtype and regulate nicotinic downstream signaling in neurons.
    Authors: Liu Z, Conroy WG, Stawicki TM, Nai Q, Neff RA, Berg DK
    Mol. Cell. Neurosci., 2008-03-18;38(2):236-44.
    Species: Chicken
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Ephrin-B reverse signaling promotes structural and functional synaptic maturation in vivo.
    Authors: Lim BK, Matsuda N, Poo MM
    Nat. Neurosci., 2008-01-13;11(2):160-9.
    Species: Xenopus
    Sample Types: In Vivo
    Applications: In Vivo
  18. Plasticity of neuron-glial interactions mediated by astrocytic EphARs.
    Authors: Nestor MW, Mok LP, Tulapurkar ME, Thompson SM
    J. Neurosci., 2007-11-21;27(47):12817-28.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  19. EphrinB3 regulates cell proliferation and survival in adult neurogenesis.
    Authors: Ricard J, Salinas J, Garcia L, Liebl DJ
    Mol. Cell. Neurosci., 2006-02-17;31(4):713-22.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  20. EphB2 and ephrin-B1 expressed in the adult kidney regulate the cytoarchitecture of medullary tubule cells through Rho family GTPases.
    Authors: Ogawa K, Wada H, Okada N, Harada I, Nakajima T, Pasquale EB, Tsuyama S
    J. Cell. Sci., 2006-02-01;119(0):559-70.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  21. EphB2/R-Ras signaling regulates glioma cell adhesion, growth, and invasion.
    Authors: Nakada M, Niska JA, Tran NL, McDonough WS, Berens ME
    Am. J. Pathol., 2005-08-01;167(2):565-76.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  22. Induction of ephrin-B1 and EphB receptors during denervation-induced plasticity in the adult mouse hippocampus.
    Authors: Wang Y, Ying GX, Liu X, Wang WY, Dong JH, Ni ZM, Zhou CF
    Eur. J. Neurosci., 2005-05-01;21(9):2336-46.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  23. The phosphorylation of EphB2 receptor regulates migration and invasion of human glioma cells.
    Authors: Nakada M, Niska JA, Miyamori H, McDonough WS, Wu J, Sato H, Berens ME
    Cancer Res., 2004-05-01;64(9):3179-85.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  24. Ephrin-B1 forward and reverse signaling are required during mouse development.
    Authors: Davy A, Aubin J, Soriano P
    Genes Dev., 2004-03-01;18(5):572-83.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  25. Control of hippocampal dendritic spine morphology through ephrin-A3/EphA4 signaling.
    Authors: Murai KK, Nguyen LN, Irie F, Yamaguchi Y, Pasquale EB
    Nat. Neurosci., 2003-02-01;6(2):153-60.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  26. Ephrin stimulation modulates T cell chemotaxis.
    Authors: Sharfe N, Freywald A, Toro A, Dadi H, Roifman C
    Eur. J. Immunol., 2002-12-01;32(12):3745-55.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Recombinant Mouse Ephrin-B1 Fc Chimera Protein, CF
By Anonymous on 04/21/2017