Recombinant Mouse FABP4/A-FABP Protein, CF

Catalog #: 9480-CL Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9480-CL-100

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Summary Product Datasheets Carrier Free Reconstitution Calculator Background Related Research Areas

Recombinant Mouse FABP4/A-FABP Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Bioassay data are not available.

Source

E. coli-derived mouse FABP4/A-FABP protein

MHHHHHH	GS	Mouse FABP4/A-FABP (Met1-Ala132) Accession # P04117
N-terminus		C-terminus

Accession #

P04117

N-terminal Sequence
Analysis

Met

Predicted Molecular Mass

16 kDa

SDS-PAGE

14 kDa, reducing conditions

Product Datasheets

9480-CL

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9480-CL

Formulation	Lyophilized from a 0.2 μm filtered solution in MES and NaCl.
Reconstitution	Reconstitute at 1 mg/mL in water.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: FABP4/A-FABP

Fatty acid binding protein-4 (FABP4; also named A- (adipocyte) FABP, ALBP, or adipocyte protein 2 (aP2)) is a member of a large superfamily of lipid binding proteins that are expressed in a tissue specific manner (1, 5, 6). FABP4 is one of ten cytoplasmic FABPs that are 14-15 kDa in size and range from 126-140 amino acids (aa) in length (1, 2, 3). Although all are highly conserved in their tertiary structure, there is only modest aa identity between any two members. The FABP family members are subdivided based on organ or tissue type it was originally expressed or identified; liver- (L-FABP), intestine- (I-FABP), heart- (H-FABP), adipocyte- (A-FABP), epidermal- (E-FABP), ileal- (Il-FABP), brain- (B-FABP), myelin- (M-FABP) and testis-FABP (T-FABP) (1). Mouse A- FABP, the product of the FABP4 gene, is a 132 aa cytosolic protein that shows a flattened beta -barrel structure generated by a series of antiparallel beta -strands and two alpha ‑helices (4, 8). FABP4 is able to bind long-chain fatty acids, retinoic acids or eicosanoids with one mol of ligand bound per mole of protein (1, 8, 9). It is suggested that ligands first bind to the outside of the molecule, and this binding subsequently induces a conformational change in the binding protein, resulting in "internalization" of the ligand (4, 7, 9). Mouse FABP4 is 92%, 94% and 91% aa identical to human, rat and canine FABP4, respectively. It also shows 26% and 28% aa identity to mouse L-FABP and I‑FABP, respectively.

References

Smathers, R & Petersen, D. (2011) Human Genomics 5:170.
Storch, J. & Thumser, AE. (2000) Biochim Biophys Acta. 1486:28.
Zimmerman, A.W. & Veerkamp, J.H. (2007) Protein Sci. 9:2042.
Bernlohr, D. et al. (1997) Ann. Rev. of Nut. 17:277.
Zimmerman, A.W. and J.H. Veerkamp (2002) Cell. Mol. Life Sci. 59:1096.
Haunerland, N.H. and F. Spener (2004) Prog. Lipid Res. 43:328.
Xu Z. et. al. (1992) Biochemistry. 31:3484.
Matarese, V. & Bernlohr, D. (1988) J Biol Chem. 263:14544.
Majava,V. et al. (2010) PLoS One. 5:e10300.

Long Name

Fatty Acid-Binding Protein 4

Entrez Gene IDs

2167 (Human); 11770 (Mouse); 79451 (Rat)

Alternate Names

Adipocyte lipid-binding protein; Adipocyte-type fatty acid-binding protein; AFABP; A-FABP; A-FABPAFABP; ALBP; aP2; FABP4; fatty acid binding protein 4, adipocyte; Fatty acid-binding protein 4; fatty acid-binding protein, adipocyte