Recombinant Mouse Fas Ligand/TNFSF6 Protein Summary
Product Specifications
Pro132-Leu279, with an N-terminal Met and 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
526-SA
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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526-SA/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: Fas Ligand/TNFSF6
Fas ligand (FasL) is a 40 kDa type II membrane protein belonging to the TNF family. In the new TNF super family nomenclature, FasL is referred to as TNFSF6. The specific receptor for FasL is Fas (CD95, Apo-1), a 45 kDa type I transmembrane protein that is a member of the TNF receptor family. FasL is predominantly expressed on activated T cells and NK cells, while Fas is expressed on various types of cells. The Fas/FasL system plays a crucial role in modulating immune response by inducing cell apoptosis to maintain homeostasis, self-tolerance of lymphocytes, and immune privilege. FasL was reported to be a potent chemoattractant for neutrophils, suggesting a novel proinflammatory function of this molecule. Like other members of the TNF family, the membrane-bound FasL can be cleaved by metalloproteinase to generate the soluble Fas ligand (sFasL) which is mainly a non-covalently linked homotrimer. It has been shown that the membrane-bound TNF‑ alpha and FasL are primary activators of their receptors. In contrast to soluble TNF‑ alpha which has potent cytotoxicity, sFasL is much less cytotoxic. Studies have shown that sFasL may competitively inhibit the killing effect of membrane FasL indicating that the cleaving of membrane FasL might be a mechanism to down‑regulate their activities.
- Suda, T. et al. (1993) Cell 75:1169.
- Kägi, D. et al. (1994) Science 265:528.
- Schneider, P. et al. (1998) J. Exp. Med. 187:1205.
- Seino, K. et al. (1998) J. Immunol. 161:4484.
Citations for Recombinant Mouse Fas Ligand/TNFSF6 Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Skeletal Muscle Is an Antigen Reservoir in Integrase-Defective Lentiviral Vector-Induced Long-Term Immunity
Authors: YY Lin, I Belle, M Blasi, MN Huang, AF Buckley, W Rountree, ME Klotman, A Cara, D Negri
Mol Ther Methods Clin Dev, 2020-03-13;17(0):532-544.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
FasL Modulates Expression of Mmp2 in Osteoblasts
Authors: E Svandova, B Vesela, H Lesot, J Sadoine, A Poliard, E Matalova
Front Physiol, 2018-09-19;9(0):1314.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Tissue inhibitor of metalloproteinases-3 facilitates Fas-mediated neuronal cell death following mild ischemia.
Authors: Wetzel M, Li L, Harms KM, Roitbak T, Ventura PB, Rosenberg GA, Khokha R, Cunningham LA
Cell Death Differ., 2007-10-26;15(1):143-51.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
The Fas/Fas ligand system inhibits differentiation of murine osteoblasts but has a limited role in osteoblast and osteoclast apoptosis.
Authors: Kovacic N, Lukic IK, Grcevic D, Katavic V, Croucher P, Marusic A
J. Immunol., 2007-03-15;178(6):3379-89.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
FAQs
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This protein datasheet indicates I need to use a cross-linking antibody, Catalog # MAB050, for biological activity. What is this antibody and is it really necessary?
The antibody is directed against a 6x histidine repeat and is recommended for use as a cross-linker of proteins with 6x his-tag. Crosslinking is often used for proteins that require receptor trimerization and can result greater biological activity. R&D Systems Quality Control tests the performance of these proteins in the presence of the cross-linking antibody. Therefore, it is necessary to use this antibody when trying to achieve the same level of specific activity described in the datasheet.
Reviews for Recombinant Mouse Fas Ligand/TNFSF6 Protein
Average Rating: 3 (Based on 1 Review)
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Reason for Rating: Product worked to induce some cytotoxicity at 2 ug/mL, however effect size was far below cytotoxic t cell activity in vitro.
rmFas ligand added at 2 ug/mL for 2 day culture.
